ID   NHAB_ACTP2              Reviewed;         513 AA.
AC   A3MZ40;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
DE   AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN   Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599}; OrderedLocusNames=APL_0322;
OS   Actinobacillus pleuropneumoniae serotype 5b (strain L20).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Actinobacillus.
OX   NCBI_TaxID=416269;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=L20;
RX   PubMed=18065534; DOI=10.1128/jb.01845-07;
RA   Foote S.J., Bosse J.T., Bouevitch A.B., Langford P.R., Young N.M.,
RA   Nash J.H.E.;
RT   "The complete genome sequence of Actinobacillus pleuropneumoniae L20
RT   (serotype 5b).";
RL   J. Bacteriol. 190:1495-1496(2008).
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC         Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01599}.
CC   -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC       family. {ECO:0000255|HAMAP-Rule:MF_01599}.
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DR   EMBL; CP000569; ABN73426.1; -; Genomic_DNA.
DR   RefSeq; WP_011848354.1; NC_009053.1.
DR   AlphaFoldDB; A3MZ40; -.
DR   STRING; 416269.APL_0322; -.
DR   EnsemblBacteria; ABN73426; ABN73426; APL_0322.
DR   KEGG; apl:APL_0322; -.
DR   PATRIC; fig|416269.6.peg.330; -.
DR   eggNOG; COG3067; Bacteria.
DR   HOGENOM; CLU_041110_0_0_6; -.
DR   OMA; QFEMLAV; -.
DR   Proteomes; UP000001432; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR   HAMAP; MF_01599; NhaB; 1.
DR   InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR   PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR   Pfam; PF06450; NhaB; 1.
DR   TIGRFAMs; TIGR00774; NhaB; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Reference proteome; Sodium; Sodium transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..513
FT                   /note="Na(+)/H(+) antiporter NhaB"
FT                   /id="PRO_0000333080"
FT   TRANSMEM        21..41
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        119..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        299..318
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        322..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        350..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        389..409
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        477..497
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
SQ   SEQUENCE   513 AA;  56633 MW;  D224F17F488B535B CRC64;
     MDSSNAIFKS FLGKAPEWYK ICIIAFLVIN PLIYFFISPF VAGWALVAEF IFTLSMALKC
     YPLQPGGLLA IEAVFIGMTS AHHVKEEIMA NFEVILLLMF MVAGIYFMKQ LLLYVFTKLL
     IVIHSKKILS LAFCLSATFL SAFLDALTVI AVIISVGTGF YGVYHKVASG NSFEDSTDIS
     NDEKIITNKE ILEQFRAFLR SPLMHAAVGS ALGGVMTMVG EPQNLIIAGQ AEWGFVEFLL
     RVLPVSLPVL ICGVITCLLL EHFKIFGYGA RLPRRVWGVL ARYNLLKEQR MTQQDRVKMG
     IQALAGIWLV VGLALHLADV GIIGLTIIII CTAFCGITDE HAIGRSFQEP MPFTALIVVF
     FTVVAVIVDL KLFEPIISYV LSADPHSQLA LFYVFNGLLS MISDNVFVGT VYINEAKTAL
     ESAIISREQF DLIAVAINTG TNLPSVATPN GQAAFLFLLT SPFAPLIRLS YGKMLYMALP
     YTIVLSIIGF LSLEFLLPPL TELMSNWGWI ITR