ID   NHAB_ECOUT              Reviewed;         513 AA.
AC   Q1RCR0;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
DE   AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN   Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599}; OrderedLocusNames=UTI89_C1372;
OS   Escherichia coli (strain UTI89 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=364106;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTI89 / UPEC;
RX   PubMed=16585510; DOI=10.1073/pnas.0600938103;
RA   Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A.,
RA   Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S.,
RA   Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J.,
RA   Gordon J.I.;
RT   "Identification of genes subject to positive selection in uropathogenic
RT   strains of Escherichia coli: a comparative genomics approach.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006).
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC         Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01599}.
CC   -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC       family. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABE06854.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000243; ABE06854.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_000406401.1; NC_007946.1.
DR   AlphaFoldDB; Q1RCR0; -.
DR   EnsemblBacteria; ABE06854; ABE06854; UTI89_C1372.
DR   KEGG; eci:UTI89_C1372; -.
DR   HOGENOM; CLU_041110_0_0_6; -.
DR   OMA; QFEMLAV; -.
DR   Proteomes; UP000001952; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR   HAMAP; MF_01599; NhaB; 1.
DR   InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR   PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR   Pfam; PF06450; NhaB; 1.
DR   TIGRFAMs; TIGR00774; NhaB; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..513
FT                   /note="Na(+)/H(+) antiporter NhaB"
FT                   /id="PRO_0000333089"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        97..117
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        144..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        238..258
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        303..323
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        348..368
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        391..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        447..467
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        475..495
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
SQ   SEQUENCE   513 AA;  56703 MW;  6D0B74EA11E06AA3 CRC64;
     MEISWGRALW RNFLGQSPDW YKLALIIFLI VNPLIFLISP FVAGWLLVAE FIFTLAMALK
     CYPLLPGGLL AIEAVFIGMT SAEHVREEVA ANLEVLLLLM FMVAGIYFMK QLLLFIFTRL
     LLSIRSKMLL SLSFCVAAAF LSAFLDALTV VAVVISVAVG FYGIYHRVAS SRTEDTDLQD
     DSHIDKHYKV VLEQFRGFLR SLMMHAGVGT ALGGVMTMVG EPQNLIIAKA AGWHFGDFFL
     RMSPVTVPVL ICGLLTCLLV EKLRWFGYGE TLPEKVREVL QQFDDQSRLQ RTRQDKIRLI
     VQAIIGVWLV TALALHLAEV GLIGLSVIIL ATSLTGVTDE HAIGKAFTES LPFTALLTVF
     FSVVAVIIDQ QLFSPIIQFV LQASEHAQLS LFYIFNGLLS SISDNVFVGT IYINEAKAAM
     KSGAITLKQY ELLAVAINTG TNLPSVATPN GQAAFLFLLT SALAPLIRLS YGRMVWMALP
     YTLVLTLVGL LCVEFTLAPV TEWFMQMGWI ATL