ID   NHAB_PSEA7              Reviewed;         500 AA.
AC   A6V701;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
DE   AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN   Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599}; OrderedLocusNames=PSPA7_3477;
OS   Pseudomonas aeruginosa (strain PA7).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=381754;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PA7;
RA   Dodson R.J., Harkins D., Paulsen I.T.;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC         Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01599}.
CC   -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC       family. {ECO:0000255|HAMAP-Rule:MF_01599}.
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DR   EMBL; CP000744; ABR81620.1; -; Genomic_DNA.
DR   RefSeq; WP_012076161.1; NC_009656.1.
DR   AlphaFoldDB; A6V701; -.
DR   SMR; A6V701; -.
DR   EnsemblBacteria; ABR81620; ABR81620; PSPA7_3477.
DR   KEGG; pap:PSPA7_3477; -.
DR   HOGENOM; CLU_041110_0_0_6; -.
DR   OMA; QFEMLAV; -.
DR   OrthoDB; 305652at2; -.
DR   Proteomes; UP000001582; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR   HAMAP; MF_01599; NhaB; 1.
DR   InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR   PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR   Pfam; PF06450; NhaB; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..500
FT                   /note="Na(+)/H(+) antiporter NhaB"
FT                   /id="PRO_0000333107"
FT   TRANSMEM        28..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        121..141
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        145..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        244..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        301..318
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        350..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        394..414
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        449..469
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        477..497
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
SQ   SEQUENCE   500 AA;  54178 MW;  3C184070177625BC CRC64;
     MSSPLSQAFA QNFLGHSPRW YKLTILAFLL SNPLLLWLAG PTVAAWVLVG EFIFTLAMAL
     KCYPLQPGGL LVLEALLLGL ASPEALYAEL QHNFPVLLLL MFMVAGIYFM KDLLLLLFSR
     LLLGVRSKTL LSLLFCLLAA LLSAFLDALT VTAVVISVAV AFFAVYHRVA SGQRASEDYD
     PATDRQVPEL HRAHLEEFRA FLRSLLMHAA VGTALGGVCT LVGEPQNLLI GHEAGWHFVE
     FFRQVAPVSM PVLAAGLLTC VALEKSRRFG YGAQLPAAVR QVLAEYAAGE SRKRGPQQKA
     ALLVQALAAL VLMIGLALHV AEVGLIGLLV IVLITAFTGI TDEHQIGRAF QEALPFTALL
     VAFFAVVAVI HQQHLFTPII QAVLALPAER QPGMLFIANG LLSAISDNVF VATIYITEVK
     QALDAGHMTR EHFDTLAVAI NTGTNLPSVA TPNGQAAFLF LLTSSIAPLV RLSYGRMVWM
     ALPYTLVMGG LGWWAVSHWL