NHAB_PSEAB
ID NHAB_PSEAB Reviewed; 500 AA.
AC Q02KW3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
DE AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599}; OrderedLocusNames=PA14_41000;
OS Pseudomonas aeruginosa (strain UCBPP-PA14).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208963;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCBPP-PA14;
RX PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT combinatorial.";
RL Genome Biol. 7:R90.1-R90.14(2006).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01599}.
CC -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01599}.
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DR EMBL; CP000438; ABJ11006.1; -; Genomic_DNA.
DR RefSeq; WP_003087967.1; NZ_CP034244.1.
DR AlphaFoldDB; Q02KW3; -.
DR SMR; Q02KW3; -.
DR PRIDE; Q02KW3; -.
DR EnsemblBacteria; ABJ11006; ABJ11006; PA14_41000.
DR KEGG; pau:PA14_41000; -.
DR HOGENOM; CLU_041110_0_0_6; -.
DR OMA; QFEMLAV; -.
DR BioCyc; PAER208963:G1G74-3433-MON; -.
DR Proteomes; UP000000653; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR HAMAP; MF_01599; NhaB; 1.
DR InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR Pfam; PF06450; NhaB; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..500
FT /note="Na(+)/H(+) antiporter NhaB"
FT /id="PRO_0000333108"
FT TRANSMEM 28..50
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 449..469
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 477..497
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
SQ SEQUENCE 500 AA; 54204 MW; 083821B6525B7D14 CRC64;
MSSPLSQAFA QNFLGHSPRW YKLTVLAFLL LNPLLLWLAG PVTSAWVLVG EFIFTLAMAL
KCYPLQPGGL LVLEALLLGL ATPEALYAEL QHNFPVLLLL MFMVAGIYFM KDLLLLLFSR
LLLGVRSKAL LSLLFCLLAA LLSAFLDALT VTAVVISVAV AFFAVYHRVA SGQRASEDYD
PATDRQVPEL HRAHLEEFRA FLRSLLMHAA VGTALGGVCT LVGEPQNLLI GHEAGWHFVE
FFRQVAPVSM PVLAAGLLTC VLLEKSRRFG YGAQLPAAVR QVLAEYAASE SRKRGAQQKA
ALLVQALAAL VLIVGLALHV AEVGLIGLLV IVLITAFTGV TDEHQIGRAF QEALPFTALL
VVFFAVVAVI HQQHLFTPII QAVLALPAER QPGMLFIANG LLSAISDNVF VATIYITEVK
QALDAGHMSR EHFDTLAVAI NTGTNLPSVA TPNGQAAFLF LLTSSIAPLV RLSYGRMVWM
ALPYTLVMGG LGWWAVSHWL
