NHAB_PSEAE
ID NHAB_PSEAE Reviewed; 500 AA.
AC Q9I2S5; Q9KWF4;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
DE AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599, ECO:0000303|PubMed:15107534};
GN OrderedLocusNames=PA1820;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, ACTIVITY REGULATION, AND
RP BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=15107534; DOI=10.1111/j.1348-0421.2004.tb03520.x;
RA Kuroda T., Fujita N., Utsugi J., Kuroda M., Mizushima T., Tsuchiya T.;
RT "A major Li(+) extrusion system NhaB of Pseudomonas aeruginosa: comparison
RT with the major Na(+) extrusion system NhaP.";
RL Microbiol. Immunol. 48:243-250(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons (PubMed:15107534). Can also transport lithium
CC (PubMed:15107534). {ECO:0000269|PubMed:15107534}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC -!- ACTIVITY REGULATION: Inhibited by amiloride.
CC {ECO:0000269|PubMed:15107534}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.3 mM for Na(+) (in the absence of amiloride, at pH 8.0)
CC {ECO:0000269|PubMed:15107534};
CC KM=2.0 mM for Li(+) (in the absence of amiloride, at pH 8.0)
CC {ECO:0000269|PubMed:15107534};
CC KM=12.5 mM for Na(+) (in the presence of 0.2 mM amiloride, at pH 8.0)
CC {ECO:0000269|PubMed:15107534};
CC KM=21.0 mM for Li(+) (in the presence of 0.2 mM amiloride, at pH 8.0)
CC {ECO:0000269|PubMed:15107534};
CC pH dependence:
CC Optimum pH is 7.5-8.5. Activity decreases sharply below pH 7.0 and is
CC null at pH 6.5. {ECO:0000269|PubMed:15107534};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01599}.
CC -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01599, ECO:0000305}.
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DR EMBL; AB037930; BAB07876.2; -; Genomic_DNA.
DR EMBL; AE004091; AAG05209.1; -; Genomic_DNA.
DR PIR; F83418; F83418.
DR RefSeq; NP_250511.1; NC_002516.2.
DR RefSeq; WP_003113594.1; NZ_QZGE01000003.1.
DR AlphaFoldDB; Q9I2S5; -.
DR SMR; Q9I2S5; -.
DR STRING; 287.DR97_60; -.
DR TCDB; 2.A.34.1.3; the nhab na(+):h(+) antiporter (nhab) family.
DR PaxDb; Q9I2S5; -.
DR EnsemblBacteria; AAG05209; AAG05209; PA1820.
DR GeneID; 877826; -.
DR KEGG; pae:PA1820; -.
DR PATRIC; fig|208964.12.peg.1890; -.
DR PseudoCAP; PA1820; -.
DR HOGENOM; CLU_041110_0_0_6; -.
DR InParanoid; Q9I2S5; -.
DR OMA; QFEMLAV; -.
DR PhylomeDB; Q9I2S5; -.
DR BioCyc; PAER208964:G1FZ6-1858-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR HAMAP; MF_01599; NhaB; 1.
DR InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR Pfam; PF06450; NhaB; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..500
FT /note="Na(+)/H(+) antiporter NhaB"
FT /id="PRO_0000333106"
FT TRANSMEM 28..50
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 449..469
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 477..497
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT CONFLICT 390
FT /note="R -> H (in Ref. 1; BAB07876)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 500 AA; 54234 MW; A93450B589FE7D12 CRC64;
MSSPLSQAFA QNFLGHSPRW YKLTVLAFLL LNPLLLWLAG PVTSAWVLVG EFIFTLAMAL
KCYPLQPGGL LVLEALLLGL ATPEALYAEL QHNFPVLLLL MFMVAGIYFM KDLLLLLFSR
LLLGVRSKTL LSLLFCLLAA LLSAFLDALT VTAVVISVAV AFFAVYHRVA SGQRASEDYD
PATDRQVPEL HRAHLEEFRA FLRSLLMHAA VGTALGGVCT LVGEPQNLLI GHEAGWHFVE
FFRQVAPVSM PVLAAGLLTC VLLEKSRRFG YGAQLPAAVR QVLAEYAASE SRKRGAQQKA
ALLVQALAAL VLIVGLALHV AEVGLIGLLV IVLITAFTGV TDEHQIGRAF QEALPFTALL
VVFFAVVAVI HQQHLFTPII QAVLALPAER QPGMLFIANG LLSAISDNVF VATIYITEVK
QALDAGHMSR EHFDTLAVAI NTGTNLPSVA TPNGQAAFLF LLTSSIAPLV RLSYGRMVWM
ALPYTLVMGG LGWWAVSHWL
