ID   NHAB_PSECL              Reviewed;         220 AA.
AC   P27763;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Nitrile hydratase subunit beta;
DE            Short=NHase;
DE            Short=Nitrilase;
DE            EC=4.2.1.84;
GN   Name=nthB;
OS   Pseudomonas chlororaphis (Pseudomonas aureofaciens).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-23 AND
RP   158-170.
RC   STRAIN=B23;
RX   PubMed=2013568; DOI=10.1128/jb.173.8.2465-2472.1991;
RA   Nishiyama M., Horinouchi S., Kobayashi M., Nagasawa T., Yamada H.,
RA   Beppu T.;
RT   "Cloning and characterization of genes responsible for metabolism of
RT   nitrile compounds from Pseudomonas chlororaphis B23.";
RL   J. Bacteriol. 173:2465-2472(1991).
CC   -!- FUNCTION: NHase catalyzes the hydration of various nitrile compounds to
CC       the corresponding amides.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an aliphatic amide = a nitrile + H2O; Xref=Rhea:RHEA:12673,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:18379, ChEBI:CHEBI:65285; EC=4.2.1.84;
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC   -!- BIOTECHNOLOGY: Industrial production of acrylamide is now being
CC       developed using some of these enzymes.
CC   -!- SIMILARITY: Belongs to the nitrile hydratase subunit beta family.
CC       {ECO:0000305}.
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DR   EMBL; D90216; BAA14246.1; -; Genomic_DNA.
DR   PIR; B42725; B42725.
DR   AlphaFoldDB; P27763; -.
DR   SMR; P27763; -.
DR   BioCyc; MetaCyc:MON-15568; -.
DR   GO; GO:0080109; F:indole-3-acetonitrile nitrile hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.472.20; -; 1.
DR   InterPro; IPR042262; CN_hydtase_beta_C.
DR   InterPro; IPR024690; CN_hydtase_beta_dom.
DR   InterPro; IPR008990; Elect_transpt_acc-like_dom_sf.
DR   InterPro; IPR003168; Nitrile_hydratase_bsu.
DR   Pfam; PF02211; NHase_beta; 1.
DR   PIRSF; PIRSF001427; NHase_beta; 1.
DR   SUPFAM; SSF50090; SSF50090; 1.
DR   TIGRFAMs; TIGR03888; nitrile_beta; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Lyase.
FT   CHAIN           1..220
FT                   /note="Nitrile hydratase subunit beta"
FT                   /id="PRO_0000186828"
SQ   SEQUENCE   220 AA;  24546 MW;  191AE1C5F14D4864 CRC64;
     MDGFHDLGGF QGFGKVPHTI NSLSYKQVFK QDWEHLAYSL MFVGVDQLKK FSVDEVRHAV
     ERLDVRQHVG TQYYERYIIA TATLLVETGV ITQAELDQAL GSHFKLANPA HATGRPAITG
     RPPFEVGDRV VVRDEYVAGH IRMPAYVRGK EGVVLHRTSE QWPFPDAIGH GDLSAAHQPT
     YHVEFRVKDL WGDAADDGYV VVDLFESYLD KAPGAQAVNA