NHAB_PSEPG
ID NHAB_PSEPG Reviewed; 500 AA.
AC B0KMR7;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
DE AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN OrderedLocusNames=PputGB1_3630;
OS Pseudomonas putida (strain GB-1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=76869;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GB-1;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Bruce D., Goodwin L., Chertkov O., Brettin T.,
RA Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M.,
RA Hauser L., Kyrpides N., Kim E., McCarthy J.K., Richardson P.;
RT "Complete sequence of Pseudomonas putida GB-1.";
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01599}.
CC -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01599}.
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DR EMBL; CP000926; ABY99521.1; -; Genomic_DNA.
DR RefSeq; WP_012273232.1; NC_010322.1.
DR AlphaFoldDB; B0KMR7; -.
DR SMR; B0KMR7; -.
DR STRING; 76869.PputGB1_3630; -.
DR EnsemblBacteria; ABY99521; ABY99521; PputGB1_3630.
DR KEGG; ppg:PputGB1_3630; -.
DR eggNOG; COG3067; Bacteria.
DR HOGENOM; CLU_041110_0_0_6; -.
DR OMA; QFEMLAV; -.
DR Proteomes; UP000002157; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR HAMAP; MF_01599; NhaB; 1.
DR InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR Pfam; PF06450; NhaB; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..500
FT /note="Na(+)/H(+) antiporter NhaB"
FT /id="PRO_0000333113"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 394..414
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 477..497
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
SQ SEQUENCE 500 AA; 54435 MW; FD4520C727DAE3E5 CRC64;
MSRSLTGALA HGFLGQSPLW YKAIICLFLV LNPLLLVTVG PVAAGWALVL EFIFTLGMAL
KCYPLMPGGL LLVEALLLQM TTPQALYEEL QHNFPVILLL MFMVAGIHFM KELLLFLFSR
ILLGVRSKAI LSLLFCVLSA FLSAFLDALT VTAVIISAAV GFYAVYHRVA SGANPREDSA
LDSDQQVAQL HREDLDQFRA FLRSLLMHGA VGTALGGVCT LVGEPQNLLI GHEMGWHFAD
FFFKVAPVSL PVLGAGLLTC VLLEKLRLFG YGTLMPEPVR QVLAAYAAED DAARTQAQRI
ALWVQGLAAL ILIICLGLHV AEVGLIGLMV IVLITAFTGI TDEHRLGRAF QDAMPFTSLL
VVFFAVVAVI HQQQLFSPLI SWVLTLPTEQ QPGMLYLANG LLSAISDNVF VATIYITEVK
QAFLNGGMSR EHFETLAVAI NTGTNLPSVA TPNGQAAFLF LLTSAIAPLI RLSYGRMVWM
ALPYTVVMGG LGWWAVTYWL
