NHAB_PSEPK
ID NHAB_PSEPK Reviewed; 500 AA.
AC Q88FQ6;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
DE AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599}; OrderedLocusNames=PP_4031;
OS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS / KT2440).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=160488;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the metabolically
RT versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01599}.
CC -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE015451; AAN69623.1; -; Genomic_DNA.
DR RefSeq; NP_746159.1; NC_002947.4.
DR RefSeq; WP_010954829.1; NC_002947.4.
DR AlphaFoldDB; Q88FQ6; -.
DR STRING; 160488.PP_4031; -.
DR EnsemblBacteria; AAN69623; AAN69623; PP_4031.
DR KEGG; ppu:PP_4031; -.
DR PATRIC; fig|160488.4.peg.4284; -.
DR eggNOG; COG3067; Bacteria.
DR HOGENOM; CLU_041110_0_0_6; -.
DR OMA; QFEMLAV; -.
DR PhylomeDB; Q88FQ6; -.
DR BioCyc; PPUT160488:G1G01-4296-MON; -.
DR Proteomes; UP000000556; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR HAMAP; MF_01599; NhaB; 1.
DR InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR Pfam; PF06450; NhaB; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..500
FT /note="Na(+)/H(+) antiporter NhaB"
FT /id="PRO_0000333114"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 477..497
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
SQ SEQUENCE 500 AA; 54560 MW; B8A341993C4A05B7 CRC64;
MSRPLTGALA HGFLGKSPIW YKVVICLFLV LNPLLLVTIG PVATGWALVL EFIFTLAMAL
KCYPLMPGGL LLIEALLLQM TTPQALYEEL QHNFPVILLL MFMVAGIHFM KELLLFLFSR
ILLGVRSKAI LALLFCVLSA FLSAFLDALT VTAVIISAAV GFYAVYHRVA SGANPREDSA
LESDQQVAHL HREDLDQFRA FLRSLLMHGA VGTALGGVCT LVGEPQNLLI GHEMGWHFVD
FFLKVAPVSL PVLGAGLVTC VLLEKVRLFG YGTLMPEPVR RVLTAYAAED DAARTQAQRI
ALWVQGLAAL ILIICLGLHV AEVGLIGLMV IVLITAFTGI TDEHRLGRAF QDAMPFTSLL
VVFFAVVAVI HQQQLFSPLI NWVLALPAEQ QPGMLYLANG LLSAISDNVF VATIYITEVK
QAFLDGSMSR EHFETLAVAI NTGTNLPSVA TPNGQAAFLF LLTSAIAPLI RLSYGRMVWM
ALPYTVVMGG LGWWAVTYWL
