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NHAB_PSEPU
ID   NHAB_PSEPU              Reviewed;         217 AA.
AC   P97052;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Nitrile hydratase subunit beta;
DE            Short=NHase;
DE            Short=Nitrilase;
DE            EC=4.2.1.84;
GN   Name=nthB;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NRRL 18668;
RX   PubMed=9154927; DOI=10.1021/bi962794t;
RA   Payne M.S., Wu S., Fallon R.D., Tudor G., Stieglitz B., Turner I.M. Jr.,
RA   Nelson M.J.;
RT   "A stereoselective cobalt-containing nitrile hydratase.";
RL   Biochemistry 36:5447-5454(1997).
CC   -!- FUNCTION: NHase catalyzes the hydration of various nitrile compounds to
CC       the corresponding amides.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an aliphatic amide = a nitrile + H2O; Xref=Rhea:RHEA:12673,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:18379, ChEBI:CHEBI:65285; EC=4.2.1.84;
CC   -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC   -!- BIOTECHNOLOGY: Industrial production of acrylamide is now being
CC       developed using some of these enzymes.
CC   -!- SIMILARITY: Belongs to the nitrile hydratase subunit beta family.
CC       {ECO:0000305}.
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DR   EMBL; U89363; AAC18419.1; -; Genomic_DNA.
DR   AlphaFoldDB; P97052; -.
DR   SMR; P97052; -.
DR   BRENDA; 4.2.1.84; 5092.
DR   GO; GO:0080109; F:indole-3-acetonitrile nitrile hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.472.20; -; 1.
DR   InterPro; IPR042262; CN_hydtase_beta_C.
DR   InterPro; IPR024690; CN_hydtase_beta_dom.
DR   InterPro; IPR008990; Elect_transpt_acc-like_dom_sf.
DR   InterPro; IPR003168; Nitrile_hydratase_bsu.
DR   Pfam; PF02211; NHase_beta; 1.
DR   PIRSF; PIRSF001427; NHase_beta; 1.
DR   SUPFAM; SSF50090; SSF50090; 1.
DR   TIGRFAMs; TIGR03888; nitrile_beta; 1.
PE   1: Evidence at protein level;
KW   Lyase.
FT   CHAIN           1..217
FT                   /note="Nitrile hydratase subunit beta"
FT                   /id="PRO_0000186829"
SQ   SEQUENCE   217 AA;  24108 MW;  038285BEC0F3B527 CRC64;
     MNGIHDTGGA HGYGPVYREP NEPVFRYDWE KTVMSLLPAL LANANFNLDE FRHSIERMGP
     AHYLEGTYYE HWLHVFENLL VEKGVLTATE VATGKAASGK TATRVLTPAI VDDSSAPGLL
     RPGGGFSFFP VGDKVRVLNK NPVGHTRMPR YTRAKWGQWS STMVCFVTPD TAAHGKGEQP
     QHVYTVSFTS VELWGQDASS PKDTIRVDLW DDYLEPA
 
 
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