NHAB_PSEPU
ID NHAB_PSEPU Reviewed; 217 AA.
AC P97052;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Nitrile hydratase subunit beta;
DE Short=NHase;
DE Short=Nitrilase;
DE EC=4.2.1.84;
GN Name=nthB;
OS Pseudomonas putida (Arthrobacter siderocapsulatus).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NRRL 18668;
RX PubMed=9154927; DOI=10.1021/bi962794t;
RA Payne M.S., Wu S., Fallon R.D., Tudor G., Stieglitz B., Turner I.M. Jr.,
RA Nelson M.J.;
RT "A stereoselective cobalt-containing nitrile hydratase.";
RL Biochemistry 36:5447-5454(1997).
CC -!- FUNCTION: NHase catalyzes the hydration of various nitrile compounds to
CC the corresponding amides.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an aliphatic amide = a nitrile + H2O; Xref=Rhea:RHEA:12673,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:18379, ChEBI:CHEBI:65285; EC=4.2.1.84;
CC -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC -!- BIOTECHNOLOGY: Industrial production of acrylamide is now being
CC developed using some of these enzymes.
CC -!- SIMILARITY: Belongs to the nitrile hydratase subunit beta family.
CC {ECO:0000305}.
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DR EMBL; U89363; AAC18419.1; -; Genomic_DNA.
DR AlphaFoldDB; P97052; -.
DR SMR; P97052; -.
DR BRENDA; 4.2.1.84; 5092.
DR GO; GO:0080109; F:indole-3-acetonitrile nitrile hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 1.10.472.20; -; 1.
DR InterPro; IPR042262; CN_hydtase_beta_C.
DR InterPro; IPR024690; CN_hydtase_beta_dom.
DR InterPro; IPR008990; Elect_transpt_acc-like_dom_sf.
DR InterPro; IPR003168; Nitrile_hydratase_bsu.
DR Pfam; PF02211; NHase_beta; 1.
DR PIRSF; PIRSF001427; NHase_beta; 1.
DR SUPFAM; SSF50090; SSF50090; 1.
DR TIGRFAMs; TIGR03888; nitrile_beta; 1.
PE 1: Evidence at protein level;
KW Lyase.
FT CHAIN 1..217
FT /note="Nitrile hydratase subunit beta"
FT /id="PRO_0000186829"
SQ SEQUENCE 217 AA; 24108 MW; 038285BEC0F3B527 CRC64;
MNGIHDTGGA HGYGPVYREP NEPVFRYDWE KTVMSLLPAL LANANFNLDE FRHSIERMGP
AHYLEGTYYE HWLHVFENLL VEKGVLTATE VATGKAASGK TATRVLTPAI VDDSSAPGLL
RPGGGFSFFP VGDKVRVLNK NPVGHTRMPR YTRAKWGQWS STMVCFVTPD TAAHGKGEQP
QHVYTVSFTS VELWGQDASS PKDTIRVDLW DDYLEPA