ID   NHAB_SHEAM              Reviewed;         528 AA.
AC   A1S6X4;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
DE   AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN   Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599}; OrderedLocusNames=Sama_1925;
OS   Shewanella amazonensis (strain ATCC BAA-1098 / SB2B).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=326297;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1098 / SB2B;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Fredrickson J.,
RA   Richardson P.;
RT   "Complete sequence of Shewanella amazonensis SB2B.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC         Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01599}.
CC   -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC       family. {ECO:0000255|HAMAP-Rule:MF_01599}.
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DR   EMBL; CP000507; ABM00131.1; -; Genomic_DNA.
DR   RefSeq; WP_011760038.1; NC_008700.1.
DR   AlphaFoldDB; A1S6X4; -.
DR   SMR; A1S6X4; -.
DR   STRING; 326297.Sama_1925; -.
DR   EnsemblBacteria; ABM00131; ABM00131; Sama_1925.
DR   KEGG; saz:Sama_1925; -.
DR   eggNOG; COG3067; Bacteria.
DR   HOGENOM; CLU_041110_0_0_6; -.
DR   OMA; QFEMLAV; -.
DR   OrthoDB; 305652at2; -.
DR   Proteomes; UP000009175; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR   HAMAP; MF_01599; NhaB; 1.
DR   InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR   PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR   Pfam; PF06450; NhaB; 1.
DR   TIGRFAMs; TIGR00774; NhaB; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Reference proteome; Sodium; Sodium transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..528
FT                   /note="Na(+)/H(+) antiporter NhaB"
FT                   /id="PRO_0000333123"
FT   TRANSMEM        23..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        130..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        203..223
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        241..261
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        310..330
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        349..369
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        390..410
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        448..468
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        475..495
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
SQ   SEQUENCE   528 AA;  57631 MW;  F88E39EC4E73E205 CRC64;
     MPLTLGQSLF ANFLGNSPRW YKFAILSFLA INPILFYLNP FIAGWVLVIQ FIFTLAMALK
     CYPLQPGGLL AIEAVIIGMT SPTQVLHEIQ ANLEVLLLLV FMVAGIYFMK QLLLYAFTKM
     LTKVRSKIVV SMLFCIASAF LSAFLDALTV IAVIITVAVG FYSIYHKVAS GKDFSSDHDH
     TSEAPEQLNE SELESFRGFL RNLLMHAGVG TALGGVCTMV GEPQNLIIAA QANWQFGEFA
     IRMSPVTVPV FFAGILTCFI VEKFKWFGYG AKLPEAVHKI LSEYDAYEDA RRTPKDKVKL
     IVQALVGVWL IVGLAFHLAS VGLIGLSVII LNTAFNGITD EHALGKAFEE ALPFTALLAV
     FFAVVGVIID QQLFAPVIQW ALSYEGNVQL VIFYIANGLL SMVSDNVFVG TVYINEVKAA
     LLNGQITRDQ FDLLAVAINT GTNLPSVATP NGQAAFLFLL TSALAPLIRL SYGRMVMMAL
     PYTIVLSIVG ILAIESGFLT DMTQYFYDSH LINHHTAAEA AGKALGGH