ID   NHAB_SHEPC              Reviewed;         528 AA.
AC   A4Y624;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
DE   AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN   Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN   OrderedLocusNames=Sputcn32_1682;
OS   Shewanella putrefaciens (strain CN-32 / ATCC BAA-453).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=319224;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CN-32 / ATCC BAA-453;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Fredrickson J.,
RA   Tiedje J., Richardson P.;
RT   "Complete sequence of Shewanella putrefaciens CN-32.";
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC         Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01599}.
CC   -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC       family. {ECO:0000255|HAMAP-Rule:MF_01599}.
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DR   EMBL; CP000681; ABP75407.1; -; Genomic_DNA.
DR   RefSeq; WP_011919149.1; NC_009438.1.
DR   AlphaFoldDB; A4Y624; -.
DR   SMR; A4Y624; -.
DR   STRING; 319224.Sputcn32_1682; -.
DR   GeneID; 45042130; -.
DR   KEGG; spc:Sputcn32_1682; -.
DR   eggNOG; COG3067; Bacteria.
DR   HOGENOM; CLU_041110_0_0_6; -.
DR   OMA; QFEMLAV; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR   HAMAP; MF_01599; NhaB; 1.
DR   InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR   PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR   Pfam; PF06450; NhaB; 1.
DR   TIGRFAMs; TIGR00774; NhaB; 1.
PE   3: Inferred from homology;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..528
FT                   /note="Na(+)/H(+) antiporter NhaB"
FT                   /id="PRO_0000333133"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        131..165
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        204..224
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        240..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        305..325
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        359..379
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        391..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        449..469
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT   TRANSMEM        476..496
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
SQ   SEQUENCE   528 AA;  57521 MW;  40D6620F68A955A9 CRC64;
     MPLTMSQAFI GNFLGNSPKW YKIAILSFLI INPILFFYVS PFVAGWVLVL EFIFTLAMAL
     KCYPLQPGGL LAIEAVAIGM TSANQVLHEI EANLEVLLLL VFMVAGIYFM KQLLLFAFTK
     IITKVRSKIL VSLMFCLTSA FLSAFLDALT VIAVIIAVAV GFYAIYHKVA SGKDFSAVHD
     HTSESNNQLN NSELESFRGF LRNLLMHAGV GTALGGVCTM VGEPQNLIIA AQANWQFGEF
     VIRMSPVTVP VLIAGILTCL LVEKFRIFGY GAKLPDAVHK ILCDYAAHED AHRTNKDKMK
     LVIQVLVGVW LIAGLALHLA SVGLVGLSVI ILTTAFNGIT DEHALGKAFE EALPFTALLA
     VFFAVVAVII DQHLFAPVIQ WALSYEGNTQ LVIFYIANGL LSMVSDNVFV GTVYINEVKA
     ALIDGQITRD QFDLLAVAIN TGTNLPSVAT PNGQAAFLFL LTSALAPLIR LSYGRMVWMA
     LPYTIVLSVV GVLAIETGFL EQATQYFYDS HMIIHHSAKD VIAPLTSH