NHAB_VIBC3
ID NHAB_VIBC3 Reviewed; 530 AA.
AC A5F6Z3; C3M1V4;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
DE AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN OrderedLocusNames=VC0395_A1491, VC395_2016;
OS Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS O395).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=345073;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA Heidelberg J.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA Wang W., Wang J., Qian W., Li D., Wang L.;
RT "A recalibrated molecular clock and independent origins for the cholera
RT pandemic clones.";
RL PLoS ONE 3:E4053-E4053(2008).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01599}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01599}.
CC -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01599}.
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DR EMBL; CP000627; ABQ20827.1; -; Genomic_DNA.
DR EMBL; CP001235; ACP10009.1; -; Genomic_DNA.
DR RefSeq; WP_001132255.1; NZ_JAACZH010000001.1.
DR AlphaFoldDB; A5F6Z3; -.
DR STRING; 345073.VC395_2016; -.
DR EnsemblBacteria; ABQ20827; ABQ20827; VC0395_A1491.
DR GeneID; 57740534; -.
DR KEGG; vco:VC0395_A1491; -.
DR KEGG; vcr:VC395_2016; -.
DR PATRIC; fig|345073.21.peg.1949; -.
DR eggNOG; COG3067; Bacteria.
DR HOGENOM; CLU_041110_0_0_6; -.
DR OMA; QFEMLAV; -.
DR Proteomes; UP000000249; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR HAMAP; MF_01599; NhaB; 1.
DR InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR Pfam; PF06450; NhaB; 1.
DR TIGRFAMs; TIGR00774; NhaB; 1.
PE 3: Inferred from homology;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..530
FT /note="Na(+)/H(+) antiporter NhaB"
FT /id="PRO_0000333146"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 479..499
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
SQ SEQUENCE 530 AA; 57661 MW; 8FAE2CCF898CC081 CRC64;
MPMSLGNAFI KNFLGKAPDW YKVAIIAFLI INPIVFFFIN PFLAGWLLVV EFIFTLAMAL
KCYPLQPGGL LAIEAIAIGM TSPGQVKHEL VANIEVLLLL VFMVAGIYFM KQLLLFIFTK
ILLGIRSKVL LSIAFSVTAA FLSAFLDALT VIAVIISVAV GFYSIYHKVA SGQSVHSSHD
HTHDEGISEL TRDDLENYRA FLRSLLMHAG VGTALGGVTT MVGEPQNLII ADQAGWQFGE
FLLRMAPVTV PVFIAGMLTC MLVEKFRIFG YGARLPANVR QILLDFDSEE RKNRTNQDVA
KLWVQGAIAV WLIVGLALHL AAVGLIGLSV IILATAFTGV IEEHSLGKAF EEALPFTALL
AVFFSIVAVI IDQELFKPVI DAVLNVEDHG TQLALFYVAN GLLSMVSDNV FVGTVYITEV
KTALMEGMIS RDQFDLLAVA INTGTNLPSV ATPNGQAAFL FLLTSALAPL IRLSYGRMVI
MAFPYTLALS LVGFIGIMFL LEPMTEVFYS LGWISHHVAP AADALLQSGH