NHAB_VIBCH
ID NHAB_VIBCH Reviewed; 530 AA.
AC Q9KQU7; Q7B7L0;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599, ECO:0000303|PubMed:12562793};
DE AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599, ECO:0000303|PubMed:12562793};
GN OrderedLocusNames=VC_1901;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION AS ANTIPORTER.
RX PubMed=12562793; DOI=10.1128/jb.185.4.1236-1244.2003;
RA Herz K., Vimont S., Padan E., Berche P.;
RT "Roles of NhaA, NhaB, and NhaD Na(+)/H(+) antiporters in survival of Vibrio
RT cholerae in a saline environment.";
RL J. Bacteriol. 185:1236-1244(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000255|HAMAP-Rule:MF_01599,
CC ECO:0000269|PubMed:12562793}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01599}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01599}.
CC -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01599, ECO:0000305}.
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DR EMBL; AF489522; AAO37925.1; -; Genomic_DNA.
DR EMBL; AE003852; AAF95049.1; -; Genomic_DNA.
DR PIR; C82144; C82144.
DR RefSeq; NP_231535.1; NC_002505.1.
DR RefSeq; WP_001132255.1; NZ_LT906614.1.
DR AlphaFoldDB; Q9KQU7; -.
DR STRING; 243277.VC_1901; -.
DR PRIDE; Q9KQU7; -.
DR DNASU; 2613530; -.
DR EnsemblBacteria; AAF95049; AAF95049; VC_1901.
DR GeneID; 57740534; -.
DR KEGG; vch:VC_1901; -.
DR PATRIC; fig|243277.26.peg.1817; -.
DR eggNOG; COG3067; Bacteria.
DR HOGENOM; CLU_041110_0_0_6; -.
DR OMA; QFEMLAV; -.
DR BioCyc; VCHO:VC1901-MON; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR HAMAP; MF_01599; NhaB; 1.
DR InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR Pfam; PF06450; NhaB; 1.
DR TIGRFAMs; TIGR00774; NhaB; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..530
FT /note="Na(+)/H(+) antiporter NhaB"
FT /id="PRO_0000333145"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 140..160
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 205..225
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 238..258
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 479..499
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
SQ SEQUENCE 530 AA; 57661 MW; 8FAE2CCF898CC081 CRC64;
MPMSLGNAFI KNFLGKAPDW YKVAIIAFLI INPIVFFFIN PFLAGWLLVV EFIFTLAMAL
KCYPLQPGGL LAIEAIAIGM TSPGQVKHEL VANIEVLLLL VFMVAGIYFM KQLLLFIFTK
ILLGIRSKVL LSIAFSVTAA FLSAFLDALT VIAVIISVAV GFYSIYHKVA SGQSVHSSHD
HTHDEGISEL TRDDLENYRA FLRSLLMHAG VGTALGGVTT MVGEPQNLII ADQAGWQFGE
FLLRMAPVTV PVFIAGMLTC MLVEKFRIFG YGARLPANVR QILLDFDSEE RKNRTNQDVA
KLWVQGAIAV WLIVGLALHL AAVGLIGLSV IILATAFTGV IEEHSLGKAF EEALPFTALL
AVFFSIVAVI IDQELFKPVI DAVLNVEDHG TQLALFYVAN GLLSMVSDNV FVGTVYITEV
KTALMEGMIS RDQFDLLAVA INTGTNLPSV ATPNGQAAFL FLLTSALAPL IRLSYGRMVI
MAFPYTLALS LVGFIGIMFL LEPMTEVFYS LGWISHHVAP AADALLQSGH
