NHAB_VIBPA
ID NHAB_VIBPA Reviewed; 528 AA.
AC Q87N04; P96498;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Na(+)/H(+) antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599, ECO:0000303|PubMed:8651921};
DE AltName: Full=Sodium/proton antiporter NhaB {ECO:0000255|HAMAP-Rule:MF_01599};
GN Name=nhaB {ECO:0000255|HAMAP-Rule:MF_01599, ECO:0000303|PubMed:8651921};
GN OrderedLocusNames=VP2072;
OS Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=223926;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS ANTIPORTER, AND PH
RP DEPENDENCE.
RC STRAIN=AQ3334;
RX PubMed=8651921; DOI=10.1006/bbrc.1996.0820;
RA Nozaki K., Inaba K., Kuroda T., Tsuda M., Tsuchiya T.;
RT "Cloning and sequencing of the gene for Na+/H+ antiporter of Vibrio
RT parahaemolyticus.";
RL Biochem. Biophys. Res. Commun. 222:774-779(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIMD 2210633;
RX PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT distinct from that of V. cholerae.";
RL Lancet 361:743-749(2003).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=RIMD 2210633;
RX PubMed=16390457; DOI=10.1111/j.1365-2958.2005.04966.x;
RA Radchenko M.V., Waditee R., Oshimi S., Fukuhara M., Takabe T., Nakamura T.;
RT "Cloning, functional expression and primary characterization of Vibrio
RT parahaemolyticus K+/H+ antiporter genes in Escherichia coli.";
RL Mol. Microbiol. 59:651-663(2006).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons (PubMed:8651921, PubMed:16390457). Can also transport
CC lithium and potassium (PubMed:16390457). {ECO:0000269|PubMed:16390457,
CC ECO:0000269|PubMed:8651921}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 H(+)(out) + 2 Na(+)(in) = 3 H(+)(in) + 2 Na(+)(out);
CC Xref=Rhea:RHEA:29247, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29248;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01599};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 8.5. Activity is null at pH 7.0.
CC {ECO:0000269|PubMed:8651921};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01599, ECO:0000269|PubMed:16390457}; Multi-pass membrane
CC protein {ECO:0000255|HAMAP-Rule:MF_01599}.
CC -!- SIMILARITY: Belongs to the NhaB Na(+)/H(+) (TC 2.A.34) antiporter
CC family. {ECO:0000255|HAMAP-Rule:MF_01599, ECO:0000305}.
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DR EMBL; D83708; BAA12073.1; -; Genomic_DNA.
DR EMBL; BA000031; BAC60335.1; -; Genomic_DNA.
DR PIR; JC4814; JC4814.
DR RefSeq; NP_798451.1; NC_004603.1.
DR RefSeq; WP_005482404.1; NC_004603.1.
DR AlphaFoldDB; Q87N04; -.
DR SMR; Q87N04; -.
DR STRING; 223926.28807065; -.
DR TCDB; 2.A.34.1.2; the nhab na(+):h(+) antiporter (nhab) family.
DR EnsemblBacteria; BAC60335; BAC60335; BAC60335.
DR GeneID; 1189583; -.
DR KEGG; vpa:VP2072; -.
DR PATRIC; fig|223926.6.peg.1982; -.
DR eggNOG; COG3067; Bacteria.
DR HOGENOM; CLU_041110_0_0_6; -.
DR OMA; QFEMLAV; -.
DR Proteomes; UP000002493; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR HAMAP; MF_01599; NhaB; 1.
DR InterPro; IPR004671; Na+/H+_antiporter_NhaB.
DR PANTHER; PTHR43302:SF1; PTHR43302:SF1; 1.
DR Pfam; PF06450; NhaB; 1.
DR TIGRFAMs; TIGR00774; NhaB; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..528
FT /note="Na(+)/H(+) antiporter NhaB"
FT /id="PRO_0000333149"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT TRANSMEM 479..499
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01599"
FT CONFLICT 273
FT /note="K -> Q (in Ref. 1; BAA12073)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 528 AA; 57165 MW; 91900139D6034B4E CRC64;
MPISLGNAFI KNFLGKAPDW YKVAIIAFLI INPIVFFLIN PFVAGWLLVA EFIFTLAMAL
KCYPLQPGGL LAIEAIAIGM TSPAQVKHEL VANIEVLLLL VFMVAGIYFM KQLLLFIFTK
ILLGIRSKTL LSLAFCFAAA FLSAFLDALT VIAVVISVAV GFYSIYHKVA SGNPIGDHDH
TQDDTITELT RDDLENYRAF LRSLLMHAGV GTALGGVTTM VGEPQNLIIA DQAGWLFGEF
LIRMSPVTLP VFICGLITCA LVEKLKVFGY GAKLPDNVRQ ILVDFDREER KTRTNQDVAK
LWVQGIIAVW LIVALALHLA AVGLIGLSVI ILATSFTGVI EEHSMGKAFE EALPFTALLA
VFFSIVAVII DQELFKPVID AVLAVEDKGT QLALFYVANG LLSMVSDNVF VGTVYINEVK
SALMEGLITR EQFDLLAVAI NTGTNLPSVA TPNGQAAFLF LLTSALAPLI RLSYGRMVVM
ALPYTVVLAI VGLMGIMFFL EPATASFYDA GWIAPHTGDL TPVVSGGH
