NHAC_ALKPO
ID NHAC_ALKPO Reviewed; 462 AA.
AC P27611; D3FYJ2;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 3.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Na(+)/H(+) antiporter NhaC;
DE AltName: Full=Sodium/hydrogen antiporter;
DE AltName: Full=Sodium/proton antiporter;
GN Name=nhaC; OrderedLocusNames=BpOF4_13965;
OS Alkalihalophilus pseudofirmus (strain ATCC BAA-2126 / JCM 17055 / OF4)
OS (Bacillus pseudofirmus).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalophilus.
OX NCBI_TaxID=398511;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX PubMed=1660475; DOI=10.1016/s0021-9258(18)54523-3;
RA Ivey D.M., Guffanti A.A., Bossewitch J.S., Padan E., Krulwich T.A.;
RT "Molecular cloning and sequencing of a gene from alkaliphilic Bacillus
RT firmus OF4 that functionally complements an Escherichia coli strain
RT carrying a deletion in the nhaA Na+/H+ antiporter gene.";
RL J. Biol. Chem. 266:23483-23489(1991).
RN [2]
RP SEQUENCE REVISION, AND FUNCTION.
RC STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX PubMed=9190799; DOI=10.1128/jb.179.12.3851-3857.1997;
RA Ito M., Guffanti A.A., Zemsky J., Ivey D.M., Krulwich T.A.;
RT "Role of the nhaC-encoded Na+/H+ antiporter of alkaliphilic Bacillus firmus
RT OF4.";
RL J. Bacteriol. 179:3851-3857(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-2126 / JCM 17055 / OF4;
RX PubMed=21951522; DOI=10.1111/j.1462-2920.2011.02591.x;
RA Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J.,
RA Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B.,
RA Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G., Xue Y., Ma Y.,
RA Hu F.Z., Krulwich T.A.;
RT "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that
RT support the ability to grow in an external pH range from 7.5 to 11.4.";
RL Environ. Microbiol. 13:3289-3309(2011).
CC -!- FUNCTION: Na(+)/H(+) antiporter. Involved in pH homeostasis and sodium
CC extrusion. {ECO:0000269|PubMed:1660475, ECO:0000269|PubMed:9190799}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1660475};
CC Multi-pass membrane protein {ECO:0000269|PubMed:1660475}.
CC -!- SIMILARITY: Belongs to the NhaC Na(+)/H(+) (TC 2.A.35) antiporter
CC family. {ECO:0000305}.
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DR EMBL; U61539; AAC45432.1; -; Genomic_DNA.
DR EMBL; CP001878; ADC50844.1; -; Genomic_DNA.
DR PIR; A41594; A41594.
DR RefSeq; WP_012958206.1; NC_013791.2.
DR AlphaFoldDB; P27611; -.
DR STRING; 398511.BpOF4_13965; -.
DR TCDB; 2.A.35.1.1; the nhac na(+):h(+) antiporter (nhac) family.
DR EnsemblBacteria; ADC50844; ADC50844; BpOF4_13965.
DR KEGG; bpf:BpOF4_13965; -.
DR eggNOG; COG1757; Bacteria.
DR HOGENOM; CLU_033405_1_0_9; -.
DR OMA; NTCGAYQ; -.
DR OrthoDB; 580055at2; -.
DR Proteomes; UP000001544; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR004770; Na/H_antiport_NhaC.
DR InterPro; IPR018461; Na/H_Antiport_NhaC-like_C.
DR Pfam; PF03553; Na_H_antiporter; 1.
DR TIGRFAMs; TIGR00931; antiport_nhaC; 1.
PE 3: Inferred from homology;
KW Antiport; Cell membrane; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..462
FT /note="Na(+)/H(+) antiporter NhaC"
FT /id="PRO_0000052415"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..25
FT /note="Helical; Name=M1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 26..31
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..50
FT /note="Helical; Name=M2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 51..71
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..91
FT /note="Helical; Name=M3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..97
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical; Name=M4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..145
FT /note="Helical; Name=M5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..188
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..208
FT /note="Helical; Name=M6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..228
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..248
FT /note="Helical; Name=M7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..250
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..271
FT /note="Helical; Name=M8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..306
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..326
FT /note="Helical; Name=M9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..353
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..373
FT /note="Helical; Name=M10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374..401
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 402..421
FT /note="Helical; Name=M11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 422..429
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical; Name=M12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 451..462
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 23
FT /note="T -> A (in Ref. 1; AAC45432)"
FT /evidence="ECO:0000305"
FT CONFLICT 278
FT /note="A -> V (in Ref. 1; AAC45432)"
FT /evidence="ECO:0000305"
FT CONFLICT 339..354
FT /note="LTRKLSSRGHLIAATA -> ANKKIIFTWTFDCSDS (in Ref. 1;
FT AAC45432)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 462 AA; 49362 MW; 11ECFBD9DBD6A3F9 CRC64;
MEKKATVGFT LLLLGVMMAI IITSMFVLKV EPHIPLVVCL LIAAVFGRYL RVGWNDLESA
MIDGLKIGIK PIFILALVGI VIAVWMMSGT VPTLLFYGLS IISPEWFAVS TLLICMIVSS
FTGSSFTTVG TIGVAMMGIG TALGIDPAIA AGAVVCGACF GDKMSPLSDT TNFAPGIVGV
DLFDHIRHLL WTTVPSFVIT VILFLMIGRS QATSTTQDIQ AMLTALDSQF TLSVLTLLSP
LLVVILAMRR FPTIPVLVVG ILTGIITAAF VQGNADVARW FTIIQNGFSM ETGNEVIDGI
VNRGGLQSMM WSISLVMIAL TLGGVIQRIG VIDTLLQSLT RKLSSRGHLI AATATSSIGV
NVVTGEQYLS ILLPGKTFES FYTKLNVAKK NLSRTLEDAG TLVNPLIPWG VSGAFFASTL
GVDVIDYLPF AFFLFLSPFM TILFGYLGIG VGKEQSLANK RG
