ID   NHAD_ALKAM              Reviewed;         483 AA.
AC   Q56EB3;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Na(+)/H(+) antiporter NhaD;
DE   AltName: Full=Sodium/proton antiporter NhaD;
GN   Name=nhaD;
OS   Alkalimonas amylolytica.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alkalimonas.
OX   NCBI_TaxID=152573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLY-330.
RX   PubMed=16267283; DOI=10.1128/jb.187.22.7589-7595.2005;
RA   Liu J., Xue Y., Wang Q., Wei Y., Swartz T.H., Hicks D.B., Ito M., Ma Y.,
RA   Krulwich T.A.;
RT   "The activity profile of the NhaD-type Na+(Li+)/H+ antiporter from the soda
RT   lake haloalkaliphile Alkalimonas amylolytica is adaptive for the extreme
RT   environment.";
RL   J. Bacteriol. 187:7589-7595(2005).
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. Can also transport lithium. Has a low antiport
CC       activity that functions over a large range of sodium concentrations.
CC       {ECO:0000269|PubMed:16267283}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is at least 9.5, the highest pH value tested. No activity
CC         is observed below pH 8.5. {ECO:0000269|PubMed:16267283};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:16267283}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:16267283}.
CC   -!- SIMILARITY: Belongs to the NhaD Na(+)/H(+) (TC 2.A.62) antiporter
CC       family. {ECO:0000305}.
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DR   EMBL; AY962404; AAX63482.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q56EB3; -.
DR   STRING; 152573.SAMN04488051_10234; -.
DR   TCDB; 2.A.62.1.2; the nhad na(+):h(+) antiporter (nhad) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR004680; Cit_transptr-like_dom.
DR   InterPro; IPR045016; NhaD-like.
DR   PANTHER; PTHR43269; PTHR43269; 1.
DR   Pfam; PF03600; CitMHS; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..483
FT                   /note="Na(+)/H(+) antiporter NhaD"
FT                   /id="PRO_0000423659"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        34..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        63..83
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        136..156
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        221..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        282..302
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        349..369
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        386..406
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        416..436
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        456..476
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         330
FT                   /note="G->S: Shows an altered activity profile in both pH
FT                   and substrate concentration."
FT                   /evidence="ECO:0000269|PubMed:16267283"
SQ   SEQUENCE   483 AA;  53445 MW;  5A80457EC0556557 CRC64;
     MQSLRCVSWL AGLLCLLFST PVFAASAAPL DLTSSLVGFV CIAIFVVAYV LVMGEEKLHM
     RKSKPVLVAA GLIWILIGWV YISRDIPDVT EAAFRHNLLE FAELMLFLLV AMTYINALEE
     RRLFDALRAW MIRKGFSYQN LFWITGFLSF FISPIADNLT TALLMCAVVM KVAEGDKRFI
     NLCCVNIVIA ANAGGAFSPF GDITTLMVWQ AGLVRIDEFL VLFFPALVNY LIPAAVMSFF
     VEKRQPSAVY EDVELKRGAL RILTLFLLTV ATAVLCHSLL HLPPVLGMMM GLGYLQFFGY
     FLRMTLPGSL ARKRAMAERE GDQEKLKRLG GVVPFDVFSR VSRAEWDTLL FFYGIVMCVG
     GLGFLGYLGL MSDLLYEGWN PTSANILLGV ISAVIDNIPV MFAVLAMQPE MSHGHWLLIT
     LTAGVGGSLL SIGSAAGVAL MGQARGYYTF FGHLKWAPVI FIGYIASIAV HLWLNADLFH
     IYD