NHAD_HALED
ID NHAD_HALED Reviewed; 495 AA.
AC E1VBT7; Q2WBH9;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 1.
DT 25-MAY-2022, entry version 45.
DE RecName: Full=Na(+)/H(+) antiporter NhaD;
DE AltName: Full=Sodium/proton antiporter NhaD;
GN Name=nhaD; OrderedLocusNames=HELO_2323;
OS Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198
OS / 1H9).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Halomonas.
OX NCBI_TaxID=768066;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS AN ANTIPORTER, AND
RP INDUCTION.
RC STRAIN=ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9;
RX PubMed=16872527; DOI=10.1186/1746-1448-2-10;
RA Kurz M., Brunig A.N., Galinski E.A.;
RT "NhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress
RT response mechanism in marine habitats?";
RL Saline Syst. 2:10-10(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9;
RX PubMed=20849449; DOI=10.1111/j.1462-2920.2010.02336.x;
RA Schwibbert K., Marin-Sanguino A., Bagyan I., Heidrich G., Lentzen G.,
RA Seitz H., Rampp M., Schuster S.C., Klenk H.P., Pfeiffer F., Oesterhelt D.,
RA Kunte H.J.;
RT "A blueprint of ectoine metabolism from the genome of the industrial
RT producer Halomonas elongata DSM 2581(T).";
RL Environ. Microbiol. 13:1973-1994(2011).
CC -!- FUNCTION: Na(+)/H(+) antiporter that imports sodium into the cell in
CC exchange for protons. Can also transport lithium. May be involved in
CC adaptation to marine habitats. {ECO:0000269|PubMed:16872527}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- INDUCTION: Induced by hyperosmotic shock.
CC {ECO:0000269|PubMed:16872527}.
CC -!- SIMILARITY: Belongs to the NhaD Na(+)/H(+) (TC 2.A.62) antiporter
CC family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-72 is the initiator.
CC {ECO:0000305|PubMed:16872527}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAJ44469.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AM167899; CAJ44469.1; ALT_INIT; Genomic_DNA.
DR EMBL; FN869568; CBV42207.1; -; Genomic_DNA.
DR AlphaFoldDB; E1VBT7; -.
DR SMR; E1VBT7; -.
DR STRING; 768066.HELO_2323; -.
DR TCDB; 2.A.62.1.4; the nhad na(+):h(+) antiporter (nhad) family.
DR EnsemblBacteria; CBV42207; CBV42207; HELO_2323.
DR KEGG; hel:HELO_2323; -.
DR eggNOG; COG1055; Bacteria.
DR HOGENOM; CLU_029697_0_0_6; -.
DR OMA; RFINLCC; -.
DR Proteomes; UP000008707; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR004680; Cit_transptr-like_dom.
DR InterPro; IPR045016; NhaD-like.
DR PANTHER; PTHR43269; PTHR43269; 1.
DR Pfam; PF03600; CitMHS; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..495
FT /note="Na(+)/H(+) antiporter NhaD"
FT /id="PRO_0000423660"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 428..448
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..488
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 166
FT /note="T -> P (in Ref. 1; CAJ44469)"
FT /evidence="ECO:0000305"
FT CONFLICT 169
FT /note="N -> D (in Ref. 1; CAJ44469)"
FT /evidence="ECO:0000305"
FT CONFLICT 175
FT /note="M -> L (in Ref. 1; CAJ44469)"
FT /evidence="ECO:0000305"
FT CONFLICT 182
FT /note="L -> T (in Ref. 1; CAJ44469)"
FT /evidence="ECO:0000305"
FT CONFLICT 242
FT /note="Y -> H (in Ref. 1; CAJ44469)"
FT /evidence="ECO:0000305"
FT CONFLICT 382
FT /note="L -> V (in Ref. 1; CAJ44469)"
FT /evidence="ECO:0000305"
FT CONFLICT 399..400
FT /note="VL -> SA (in Ref. 1; CAJ44469)"
FT /evidence="ECO:0000305"
FT CONFLICT 479..495
FT /note="SVVVHLMINADSFAIFH -> VWSST (in Ref. 1; CAJ44469)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 495 AA; 54941 MW; 9FC219BA98DDD181 CRC64;
MLTNHRSPHW LRHSARWPGF LVLALPILLF SPLAQAASAG ELDLTSSLPG FIAVTLFLAA
YVLVMAEEKL HMRKSKPVLV AAGLIWAMIG WVYVHAGLPD ASEEAFSETL LEYSELLLFL
LVAMTYINAM EERRVFDKLR AWLVEKGFSY RSLFWITGIL AFWISTIANN LTTAMLMCAV
VLKVAEGDKR FINLCCINVV VASNAGGAFS PFGDITTLMV WQAKLVEFQE FFELLGPALV
NYLVPAIVMS LFIKNRKPAA LEEHIWLKRG ARRIVLLFLV TIVISVLCHT MLNLPPALGM
MTGLGFLQFF GYYLRQSLPR SLERKRTRYS QRGDWRKLES LGSVVPFDVF TRIARSEWDT
LLFFYGVVMS VGGLGFMGYL ALLSETLYTG WDPVWANIVL GLVSSVVDNI PVMFAVISME
PDMSMGNWLL ITLTAGVGGS LLSVGSAAGV ALMGQARGIY TFASHMRWAP VIALGYVASV
VVHLMINADS FAIFH
