ID   NHAG_BACAT              Reviewed;         524 AA.
AC   Q9LCB5;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Na(+)/H(+) antiporter NhaG;
DE   AltName: Full=Sodium/proton antiporter NhaG;
GN   Name=nhaG;
OS   Bacillus atrophaeus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1452;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 9372 / DSM 675 / NBRC 13721 / NCIMB 8058 / NRS 1221A;
RX   PubMed=11686935; DOI=10.1093/oxfordjournals.jbchem.a003038;
RA   Gouda T., Kuroda M., Hiramatsu T., Nozaki K., Kuroda T., Mizushima T.,
RA   Tsuchiya T.;
RT   "nhaG Na(+)/H(+) antiporter gene of Bacillus subtilis ATCC9372, which is
RT   missing in the complete genome sequence of strain 168, and properties of
RT   the antiporter.";
RL   J. Biochem. 130:711-717(2001).
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. Can also transport lithium.
CC       {ECO:0000269|PubMed:11686935}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 8.0-9.0 for sodium transport and 8.0 for lithium
CC         transport. {ECO:0000269|PubMed:11686935};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11686935};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:11686935}.
CC   -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC       transporter (TC 2.A.36) family. {ECO:0000305}.
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DR   EMBL; AB029555; BAA89487.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9LCB5; -.
DR   SMR; Q9LCB5; -.
DR   TCDB; 2.A.36.6.2; the monovalent cation:proton antiporter-1 (cpa1) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR006153; Cation/H_exchanger.
DR   InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR   PANTHER; PTHR10110; PTHR10110; 1.
DR   Pfam; PF00999; Na_H_Exchanger; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell membrane; Ion transport; Membrane; Sodium; Sodium transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..524
FT                   /note="Na(+)/H(+) antiporter NhaG"
FT                   /id="PRO_0000423857"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        126..146
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        193..213
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        242..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        283..303
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        312..332
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        374..394
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   524 AA;  58106 MW;  4083D30B1265ADE1 CRC64;
     MEHLDLHHIF ELGFFVVMIA AGITAIAKKC RQPYPIALVI VGTIIGLVHI PLFEPLKEFI
     TEGEVFNFVI ITLFLPALLG EAALKLPFSH LRENKRPVLA LFGGTLISFL IVGFSSMWLM
     HLAIPAAFVF AALMSATDPV SVLSIFKSVG APKKLSIVVE GESLFNDGLA VVLFNISAFY
     LMTYLDLGIQ GAGLGLWEFV KVISLGLIIG GVLGYVFSQL TKYFDDYPLE IIFSIILFYS
     SFLLAEMAGA SGVIAVVVAA LIFGNYGAKI GMSPTTKLNI NNFWDVAALL ANSLVFLMVG
     LEITRIDLTD KWGLAIMAIV IVLIARSAAV YISLAFIKKF PVTWKHTINW GGLKGSLSIA
     LVLSLPRDFP GREDILVFAF SVVLFSLVVQ GLTIKPLLER LGVNQKEEGN QEYEELLAKG
     HRLETAIKEV QQVKHNLLIH EAVSSELTDQ YKKEVSQLHQ QTNKLFETYP ELKNKQQTIL
     KKHSLYAQYQ AIENLSREDI ISNEVAELEQ ARIIDEIVRL QNDH