NHAH_HALDA
ID NHAH_HALDA Reviewed; 403 AA.
AC Q2XWL3;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 25-MAY-2022, entry version 52.
DE RecName: Full=Na(+)/H(+) antiporter NhaH;
DE AltName: Full=Sodium/proton antiporter NhaH;
GN Name=nhaH;
OS Halobacillus dabanensis.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Halobacillus.
OX NCBI_TaxID=240302;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=DSM 18199 / CIP 109012 / JCM 12772 / D-8;
RX PubMed=16436066; DOI=10.1111/j.1574-6968.2005.00055.x;
RA Yang L.F., Jiang J.Q., Zhao B.S., Zhang B., Feng D.Q., Lu W.D., Wang L.,
RA Yang S.S.;
RT "A Na+/H+ antiporter gene of the moderately halophilic bacterium
RT Halobacillus dabanensis D-8T: cloning and molecular characterization.";
RL FEMS Microbiol. Lett. 255:89-95(2006).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF HIS-87; HIS-88; ASP-137;
RP GLU-161; ASP-166; ASP-224; GLU-229 AND ARG-325.
RC STRAIN=DSM 18199 / CIP 109012 / JCM 12772 / D-8;
RX PubMed=23196349; DOI=10.1016/j.bbamem.2012.11.015;
RA Jiang J., Wang L., Zou Y., Lu W., Zhao B., Zhang B., Yang S., Yang L.;
RT "Identification of important charged residues for alkali cation exchange or
RT pH regulation of NhaH, a Na(+)/H(+) antiporter of Halobacillus
RT dabanensis.";
RL Biochim. Biophys. Acta 1828:997-1003(2013).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. Can also transport lithium.
CC {ECO:0000269|PubMed:16436066, ECO:0000269|PubMed:23196349}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.83 mM for sodium {ECO:0000269|PubMed:16436066};
CC KM=2.14 mM for lithium {ECO:0000269|PubMed:16436066};
CC pH dependence:
CC Optimum pH is 8.5-9.0 for sodium transport and 8.5 for lithium
CC transport. Na(+)/H(+) antiporter activity is detected between pH 6.5
CC and 9.5, whereas Li(+)/H(+) antiporter activity is detected only
CC above pH 7.5. {ECO:0000269|PubMed:16436066};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16436066,
CC ECO:0000269|PubMed:23196349}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16436066, ECO:0000269|PubMed:23196349}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. {ECO:0000305}.
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DR EMBL; DQ167194; ABA03152.1; -; Genomic_DNA.
DR RefSeq; WP_075036418.1; NZ_FOSB01000005.1.
DR AlphaFoldDB; Q2XWL3; -.
DR SMR; Q2XWL3; -.
DR TCDB; 2.A.36.6.7; the monovalent cation:proton antiporter-1 (cpa1) family.
DR eggNOG; COG0025; Bacteria.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell membrane; Ion transport; Membrane; Sodium; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..403
FT /note="Na(+)/H(+) antiporter NhaH"
FT /id="PRO_0000423859"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..30
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 52..65
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 87..98
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..124
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..167
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..195
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..218
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..241
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 263..281
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..310
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 332..372
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 394..403
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MUTAGEN 87
FT /note="H->A: Causes acidic shift of pH profile."
FT /evidence="ECO:0000269|PubMed:23196349"
FT MUTAGEN 88
FT /note="H->A: Causes acidic shift of pH profile."
FT /evidence="ECO:0000269|PubMed:23196349"
FT MUTAGEN 137
FT /note="D->A: Lack of activity."
FT /evidence="ECO:0000269|PubMed:23196349"
FT MUTAGEN 137
FT /note="D->E: Decreases activity. Increases Km for Na(+) and
FT Li(+), but does not affect pH profile."
FT /evidence="ECO:0000269|PubMed:23196349"
FT MUTAGEN 161
FT /note="E->N: Decreases activity. Increases Km for Na(+) and
FT Li(+)."
FT /evidence="ECO:0000269|PubMed:23196349"
FT MUTAGEN 166
FT /note="D->A: Lack of activity."
FT /evidence="ECO:0000269|PubMed:23196349"
FT MUTAGEN 166
FT /note="D->E: Decreases activity. Increases Km for Na(+) and
FT Li(+), and causes alkaline shift of pH profile."
FT /evidence="ECO:0000269|PubMed:23196349"
FT MUTAGEN 224
FT /note="D->A,E: Decreases activity. Increases Km for Na(+)
FT and Li(+)."
FT /evidence="ECO:0000269|PubMed:23196349"
FT MUTAGEN 229
FT /note="E->K: Decreases over 50% Km for Li(+), without
FT affecting Km for Na(+)."
FT /evidence="ECO:0000269|PubMed:23196349"
FT MUTAGEN 325
FT /note="R->A: Lack of activity."
FT /evidence="ECO:0000269|PubMed:23196349"
SQ SEQUENCE 403 AA; 43533 MW; 199CE142CFBB7D35 CRC64;
MHGFHDVFIQ ILLLLAISVS VIAIAKLLKE PDSIALVLVG LVLGLTELPI IEDAERYITQ
SEVFQATIIS LFLPILLGDA TLKLPFHHLF SQKKTVLGLA FVGTFVSSIC IGTAAYFLLD
LPLAVAFTFA ALMSATDPIS VLSIFKSLGV PQKMSTVMEG ESLFNDGIAV VLFKIASIYL
LTYMEMGWAG LGSGVFLFLK FAIGGALVGL VLGYFFSQVI RVFDDYPLEV AFSALLFFGS
YFIAEHFHTS GVIAVVVGGF VFGDYGAKIG MSKETKTNIN TFWDSVTLIA NALIFLMVGL
EIRNIDLAGN WGVIVGAILI VLVGRTIAVY LGTGWVQELS SKERLLINWG GLRGSLSVAL
ALSLPMDFAG RDQVLLLTFS VVLFSLIVQG LTLKPLIKKL GMI
