NHAK_BACSU
ID NHAK_BACSU Reviewed; 670 AA.
AC O32212;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Sodium, potassium, lithium and rubidium/H(+) antiporter;
GN Name=nhaK; Synonyms=yvgP; OrderedLocusNames=BSU33420;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [2]
RP FUNCTION IN MONOVALENT CATION EFFLUX, AND INDUCTION.
RC STRAIN=BD99 / MS94;
RX PubMed=16021482; DOI=10.1007/s00203-005-0011-6;
RA Fujisawa M., Kusumoto A., Wada Y., Tsuchiya T., Ito M.;
RT "NhaK, a novel monovalent cation/H+ antiporter of Bacillus subtilis.";
RL Arch. Microbiol. 183:411-420(2005).
CC -!- FUNCTION: Transporter involved in the efflux of sodium, potassium,
CC lithium and rubidium. {ECO:0000269|PubMed:16021482}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Constitutively expressed, with higher levels at stationary
CC phase. {ECO:0000269|PubMed:16021482}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. Nhak (TC 2.A.36.3.2) subfamily.
CC {ECO:0000305}.
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DR EMBL; AL009126; CAB15347.1; -; Genomic_DNA.
DR PIR; E70040; E70040.
DR RefSeq; NP_391222.1; NC_000964.3.
DR RefSeq; WP_003243269.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; O32212; -.
DR SMR; O32212; -.
DR IntAct; O32212; 6.
DR STRING; 224308.BSU33420; -.
DR TCDB; 2.A.36.3.2; the monovalent cation:proton antiporter-1 (cpa1) family.
DR PaxDb; O32212; -.
DR PRIDE; O32212; -.
DR EnsemblBacteria; CAB15347; CAB15347; BSU_33420.
DR GeneID; 936025; -.
DR KEGG; bsu:BSU33420; -.
DR PATRIC; fig|224308.179.peg.3627; -.
DR eggNOG; COG0025; Bacteria.
DR InParanoid; O32212; -.
DR OMA; TYNTEHC; -.
DR PhylomeDB; O32212; -.
DR BioCyc; BSUB:BSU33420-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR GO; GO:0098719; P:sodium ion import across plasma membrane; IBA:GO_Central.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR InterPro; IPR004705; Cation/H_exchanger_CPA1_bac.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR TIGRFAMs; TIGR00831; a_cpa1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Lithium; Membrane; Potassium;
KW Potassium transport; Reference proteome; Sodium; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..670
FT /note="Sodium, potassium, lithium and rubidium/H(+)
FT antiporter"
FT /id="PRO_0000337074"
FT TRANSMEM 5..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 670 AA; 75228 MW; C4BA6737A4C393BC CRC64;
MDIFLVVLVL LTIIAISNIV NRFIPFIPVP LIQVALGILA ASFPQGLHFE LNTELFFVLF
IAPLLFNDGK RTPRAELWNL RAPILLLALG LVFATVIVGG YTIHWMIPAI PLAAAFGLAA
ILSPTDVVAV SALSGRVKMP KGILRLLEGE GLMNDASGLV AFKFAIAAAV TGAFSLAQAA
VSFVFISLGG LLCGVVISFL IIRFRLFLRR LGMQDVTMHM LIQILTPFVI YLAAEEIGVS
GILAVVAGGI THAVEQDRLE STMIKLQIVS SSTWNIILFI LNGLVFVILG TQIPDVISVI
FNDTAISNMK VIGYILVITF TLMLLRFLWV LFFWNGKWFF NKDQNIYKPG LRSTLLISIS
GVRGAVTLAG SFSIPYFLED GTPFPERNLI LFLAAGVILC TLVIATVVLP ILTEKEEEDE
ERNKKLLTAR RKLIKTALQT IKEDMNETNK TASLAVIAEY NEKMKNLRFQ QYTSSNRIKK
HERKVRAQGV KAEQEALMKM LERGDIPEET ANVLQERFNE LEILYANPFK VGLSKTRLKR
LMYWIFFGEH KKPEMSILNE AGLIRATRVK TAKAAIEYLE KHKTDEHKEV FLSVITFYKQ
LIFRLEHSHH ELKSSAHFEN QKLEVKLKAV QAIRNEIQTL FEEREISRDI SHELRQYIND
VEAAMLEGGE
