ID   NHAP_ALKAM              Reviewed;         574 AA.
AC   Q0ZAH6;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=K(+)/H(+) antiporter NhaP;
DE   AltName: Full=Potassium/proton antiporter NhaP;
GN   Name=nhaP;
OS   Alkalimonas amylolytica.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alkalimonas.
OX   NCBI_TaxID=152573;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   AND GENE NAME.
RC   STRAIN=N10;
RX   PubMed=17600061; DOI=10.1099/mic.0.2007/007450-0;
RA   Wei Y., Liu J., Ma Y., Krulwich T.A.;
RT   "Three putative cation/proton antiporters from the soda lake alkaliphile
RT   Alkalimonas amylolytica N10 complement an alkali-sensitive Escherichia coli
RT   mutant.";
RL   Microbiology 153:2168-2179(2007).
CC   -!- FUNCTION: K(+)/H(+) antiporter that extrudes potassium in exchange for
CC       external protons. Can also catalyze NH(4)(+)/H(+) antiport. Could have
CC       weak activity with Na(+). {ECO:0000269|PubMed:17600061}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=0.5 mM for K(+) (at pH 8.0) {ECO:0000269|PubMed:17600061};
CC       pH dependence:
CC         Optimum pH is 7.5. Exhibits K(+)/H(+) antiporter activity between pH
CC         7.5 and 9.5. {ECO:0000269|PubMed:17600061};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC       transporter (TC 2.A.36) family. {ECO:0000305}.
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DR   EMBL; DQ649020; ABG37987.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q0ZAH6; -.
DR   SMR; Q0ZAH6; -.
DR   STRING; 152573.SAMN04488051_11215; -.
DR   TCDB; 2.A.36.6.4; the monovalent cation:proton antiporter-1 (cpa1) family.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR   GO; GO:0005451; F:monovalent cation:proton antiporter activity; IEA:InterPro.
DR   GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1530.20; -; 1.
DR   Gene3D; 3.30.465.10; -; 1.
DR   Gene3D; 3.30.70.1450; -; 1.
DR   InterPro; IPR006153; Cation/H_exchanger.
DR   InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR   InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR   InterPro; IPR038770; Na+/solute_symporter_sf.
DR   InterPro; IPR030151; NhaP.
DR   InterPro; IPR006037; RCK_C.
DR   InterPro; IPR036721; RCK_C_sf.
DR   InterPro; IPR005170; Transptr-assoc_dom.
DR   PANTHER; PTHR32507:SF7; PTHR32507:SF7; 1.
DR   Pfam; PF03471; CorC_HlyC; 1.
DR   Pfam; PF00999; Na_H_Exchanger; 1.
DR   SMART; SM01091; CorC_HlyC; 1.
DR   SUPFAM; SSF56176; SSF56176; 1.
DR   PROSITE; PS51202; RCK_C; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Potassium; Potassium transport; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..574
FT                   /note="K(+)/H(+) antiporter NhaP"
FT                   /id="PRO_0000423860"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        28..48
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        191..211
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        216..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..264
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        272..292
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        302..322
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        332..352
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        361..381
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          402..484
FT                   /note="RCK C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00544"
SQ   SEQUENCE   574 AA;  62268 MW;  2C94817C6A0956F6 CRC64;
     MEAINLTILV IGVLFLISIV ATLISSRIGA PILLVFLIIG MLAGEQGLGG ITFNNPQVAF
     LIGSIALVII LFDGGMRTHP ERFRVALAPA AMLATLGVVV TCTVTGLAAA WILGLHWLQG
     LLLGAILSST DAAAVFSIFQ SRGIRIKDRV ASTLEIESGS NDPMAVMLTI TLVGVLAEYT
     ALDWSVLIVF LKQAIIGGAV GYGAGRLFVF LCRKLPLSFA FFPLMAVACC ISVYAVTTQF
     EGSGFLAVYL MGYFVGNARL PQVLYILRVH DGLAWLSQIV MFLMLGLLVV PSQLLDHLLP
     ALAIAGVLIF IARPLAVLLS LIPFHFPAKD QLFISWVGLR GAVPIILALF PWLAGVPDEH
     LYFNVAFVIV IVSLVFQGWS ISPVARWLKL EVPKESGPDQ TMPLDAIASN EVIEVVSFTL
     KGDSPMLDKQ WQDFTVPHSA EFLGVIRDGE WLLSRDNPVF KLKDSVLVLC KMADVPDIST
     VLASAASSRT MTASDFFGDF VLNAQITLDE LDAFYSITLP EHESHVTLAD YITERFHRRV
     VVGDQVKLDA LVLTVRQLDD HGNVKLVGIK PSDS