ID   NHAP_APHHA              Reviewed;         521 AA.
AC   Q93HU4;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Na(+)/H(+) antiporter ApNhaP;
DE   AltName: Full=Sodium/proton antiporter ApNhaP;
GN   Name=apnhaP;
OS   Aphanothece halophytica.
OC   Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
OC   Aphanothecaceae; Aphanothece.
OX   NCBI_TaxID=72020;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   SUBCELLULAR LOCATION, AND DOMAIN.
RX   PubMed=11479290; DOI=10.1074/jbc.m103650200;
RA   Waditee R., Hibino T., Tanaka Y., Nakamura T., Incharoensakdi A.,
RA   Takabe T.;
RT   "Halotolerant cyanobacterium Aphanothece halophytica contains an Na(+)/H(+)
RT   antiporter, homologous to eukaryotic ones, with novel ion specificity
RT   affected by C-terminal tail.";
RL   J. Biol. Chem. 276:36931-36938(2001).
CC   -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC       external protons. Shows also high Ca(2+)/H(+) antiporter activity at
CC       alkaline pH. Does not catalyze exchange between Li(+) and H(+).
CC       {ECO:0000269|PubMed:11479290}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Active over a wide pH range between 5 and 9.
CC         {ECO:0000269|PubMed:11479290};
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:11479290}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:11479290}.
CC   -!- DOMAIN: The C-terminal tail could affect ion specificity.
CC       {ECO:0000269|PubMed:11479290}.
CC   -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC       transporter (TC 2.A.36) family. {ECO:0000305}.
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DR   EMBL; AB059562; BAB69459.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q93HU4; -.
DR   SMR; Q93HU4; -.
DR   TCDB; 2.A.36.7.1; the monovalent cation:proton antiporter-1 (cpa1) family.
DR   PRIDE; Q93HU4; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR006153; Cation/H_exchanger.
DR   InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR   InterPro; IPR004705; Cation/H_exchanger_CPA1_bac.
DR   PANTHER; PTHR10110; PTHR10110; 1.
DR   Pfam; PF00999; Na_H_Exchanger; 1.
DR   TIGRFAMs; TIGR00831; a_cpa1; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW   Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..521
FT                   /note="Na(+)/H(+) antiporter ApNhaP"
FT                   /id="PRO_0000423861"
FT   TOPO_DOM        1..18
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..41
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        63..94
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        116..126
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        127..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        148..164
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        186..194
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        195..215
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        216..245
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        267..276
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        277..297
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        298..311
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        312..332
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        333..349
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        350..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        371..380
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        402..521
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   521 AA;  56884 MW;  D15ECE05507235A7 CRC64;
     MTIEAAMGEE AIKENLEQFL IVLSVSLGVA TLSQISSFFR QIPYTLLLVI VGLGLAFVDI
     RLVNLSPELI LEIFLPPLLF EAAWNIRWRN LKKNLFPVVL LAIIGVVISV VGIGFSLNYF
     SGLSLPIALL VGAILAATDP VSVIALFREL GVGERLTVLM EGESLFNDGV AVVAFSLLVG
     IPLGTQEFSV TNTLIQFVTL QGIGIGCGGV IGFGISYLTQ RFDLPLVEQS LTLVSAYGTY
     LITEELGGSG VIGVVTVGLI LGNFGSRIGM NPRTRLLVSE FWEFIAFFVN SIVFLLIGDQ
     INIRGLADNG QLILITIIAL VIIRAISIYG LGTISNLITK QDISWQEETV LWWGGLRGSV
     SIALALSVPV MLDGRQDIIE AVFGVVLFTL LVQGLTMQTV IEKLGLIGDR AQRRTYSELI
     ARRSALERVL AHLNAVPPSP SIRMKSLKTT KEASKGQLES RQTKKLRSYN NSYPQLRSLE
     QEQLRELTFK VEADTYAELI RAGKLNNNLS PLLQEVLAKP E