NHAP_PSEAE
ID NHAP_PSEAE Reviewed; 424 AA.
AC G3XD29; O82871; Q7DC93;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2011, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=Na(+)/H(+) antiporter NhaP;
DE AltName: Full=Sodium/proton antiporter NhaP;
GN Name=nhaP; OrderedLocusNames=PA3887;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=9655928; DOI=10.1016/s0167-4781(98)00058-x;
RA Utsugi J., Inaba K., Kuroda T., Tsuda M., Tsuchiya T.;
RT "Cloning and sequencing of a novel Na+/H+ antiporter gene from Pseudomonas
RT aeruginosa.";
RL Biochim. Biophys. Acta 1398:330-334(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP FUNCTION, ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=15107534; DOI=10.1111/j.1348-0421.2004.tb03520.x;
RA Kuroda T., Fujita N., Utsugi J., Kuroda M., Mizushima T., Tsuchiya T.;
RT "A major Li(+) extrusion system NhaB of Pseudomonas aeruginosa: comparison
RT with the major Na(+) extrusion system NhaP.";
RL Microbiol. Immunol. 48:243-250(2004).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. Has also weak Li(+)/H(+) antiport activity.
CC {ECO:0000269|PubMed:15107534, ECO:0000269|PubMed:9655928}.
CC -!- ACTIVITY REGULATION: Inhibited by amiloride.
CC {ECO:0000269|PubMed:15107534}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=6.0 mM for Na(+) (in the absence of amiloride, at pH 7.5)
CC {ECO:0000269|PubMed:15107534, ECO:0000269|PubMed:9655928};
CC KM=9.2 mM for Na(+) (in the presence of 0.5 mM amiloride, at pH 7.5)
CC {ECO:0000269|PubMed:15107534, ECO:0000269|PubMed:9655928};
CC pH dependence:
CC Optimum pH is 7.5. Almost no activity is detected below pH 7.0 or
CC above pH 8.5. {ECO:0000269|PubMed:15107534,
CC ECO:0000269|PubMed:9655928};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:9655928};
CC Multi-pass membrane protein {ECO:0000269|PubMed:9655928}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. {ECO:0000305}.
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DR EMBL; AB010827; BAA31695.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG07274.1; -; Genomic_DNA.
DR PIR; C83159; C83159.
DR RefSeq; NP_252576.1; NC_002516.2.
DR RefSeq; WP_003105667.1; NZ_QZGE01000001.1.
DR AlphaFoldDB; G3XD29; -.
DR SMR; G3XD29; -.
DR STRING; 287.DR97_3981; -.
DR TCDB; 2.A.36.6.8; the monovalent cation:proton antiporter-1 (cpa1) family.
DR PaxDb; G3XD29; -.
DR PRIDE; G3XD29; -.
DR EnsemblBacteria; AAG07274; AAG07274; PA3887.
DR GeneID; 878773; -.
DR KEGG; pae:PA3887; -.
DR PATRIC; fig|208964.12.peg.4071; -.
DR PseudoCAP; PA3887; -.
DR HOGENOM; CLU_005912_8_1_6; -.
DR InParanoid; G3XD29; -.
DR OMA; SYRWPIG; -.
DR PhylomeDB; G3XD29; -.
DR BioCyc; PAER208964:G1FZ6-3960-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR GO; GO:0098719; P:sodium ion import across plasma membrane; IBA:GO_Central.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell inner membrane; Cell membrane; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..424
FT /note="Na(+)/H(+) antiporter NhaP"
FT /id="PRO_0000423862"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 424 AA; 45486 MW; 8E116A82C5825628 CRC64;
MLDLVAAFIA LTTLLTYVNY RFIRLPPTIG VMATALVFSL IVQGLSELGY PILEVEMQEI
IRRIDFSEVL MTWFLPALLF AGALHVDLSD LRSYKWPIGL LATAGVLIAT FVIGGLAYYT
FPLFGWQVDF IYCLLFGALI SPTDPIAVLG ILKSAGAPKP LATTIVGESL FNDGTAVVVF
AIILGILQLG EAPTVSATAI LFVQEAIGGV VFGAVLGYGV FVMMRGIDQY QVEVMLTLAL
VIGGAALAAR LHVSAPIAMV VAGLIIGNHG RHYAMSDETR RYVDKFWELI DEILNALLFA
LIGLELLLLP FSWLHVAAAF ALGGAVLVSR LLTVGPAILV LRRFRGANRQ VPAGTIRILV
WGGLRGGVSV ALALSLPLGP ERDLILSLTY IVVLVSILLQ GLSIGPLVRR IYAGQPLEKS
EGAH
