ID   NHAR_SHIFL              Reviewed;         301 AA.
AC   P0A9G3; P10087; P75619; Q47409; Q83SR2;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Transcriptional activator protein NhaR;
DE   AltName: Full=Na(+)/H(+) antiporter regulatory protein;
GN   Name=nhaR; OrderedLocusNames=SF0017, S0019;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Plays a role in the positive regulation of NhaA.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN41683.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=AAP15564.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE005674; AAN41683.2; ALT_INIT; Genomic_DNA.
DR   EMBL; AE014073; AAP15564.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_705976.2; NC_004337.2.
DR   RefSeq; WP_000062890.1; NZ_LM651928.1.
DR   AlphaFoldDB; P0A9G3; -.
DR   SMR; P0A9G3; -.
DR   STRING; 198214.SF0017; -.
DR   EnsemblBacteria; AAN41683; AAN41683; SF0017.
DR   EnsemblBacteria; AAP15564; AAP15564; S0019.
DR   GeneID; 1027434; -.
DR   KEGG; sfl:SF0017; -.
DR   KEGG; sfx:S0019; -.
DR   PATRIC; fig|198214.7.peg.17; -.
DR   HOGENOM; CLU_039613_9_0_6; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..301
FT                   /note="Transcriptional activator protein NhaR"
FT                   /id="PRO_0000105690"
FT   DOMAIN          6..63
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        23..42
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   301 AA;  34284 MW;  E81FAFC4FC4FD2F1 CRC64;
     MSMSHINYNH LYYFWHVYKE GSVVGAAEAL YLTPQTITGQ IRALEERLQG KLFKRKGRGL
     EPSELGELVY RYADKMFTLS QEMLDIVNYR KESNLLFDVG VADALSKRLV SSVLNAAVVE
     GEPIHLRCFE STHEMLLEQL SQHKLDMIIS DCPIDSTQQE GLFSVRIGEC GVSFWCTNPP
     PEKPFPACLE ERRLLIPGRR SMLGRKLLNW FNSQGLNVEI LGEFDDAALM KAFGAMHNAI
     FVAPTLYAYD FYADKTVVEI GRVENVMEEY HAIFAERMIQ HPAVQRICNT DYSALFSPAV
     R