NHAS1_SYNY3
ID NHAS1_SYNY3 Reviewed; 527 AA.
AC P73863;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 25-MAY-2022, entry version 133.
DE RecName: Full=Low-affinity Na(+)/H(+) antiporter NhaS1;
DE AltName: Full=Sodium/proton antiporter NhaS1;
GN Name=nhaS1; OrderedLocusNames=slr1727;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [2]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND GENE NAME.
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=11157951; DOI=10.1128/jb.183.4.1376-1384.2001;
RA Inaba M., Sakamoto A., Murata N.;
RT "Functional expression in Escherichia coli of low-affinity and high-
RT affinity Na(+)(Li(+))/H(+) antiporters of Synechocystis.";
RL J. Bacteriol. 183:1376-1384(2001).
RN [3]
RP FUNCTION.
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=11996656; DOI=10.1023/a:1015281906254;
RA Elanskaya I.V., Karandashova I.V., Bogachev A.V., Hagemann M.;
RT "Functional analysis of the Na+/H+ antiporter encoding genes of the
RT cyanobacterium Synechocystis PCC 6803.";
RL Biochemistry (Mosc.) 67:432-440(2002).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. Might be able to function at relatively high
CC concentrations of Na(+) ions. Has also Li(+)/H(+) antiport activity
CC under K(+)-rich conditions, but it might not have any physiological
CC relevance. {ECO:0000269|PubMed:11157951, ECO:0000269|PubMed:11996656}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=7.7 mM for Na(+) (under K(+)-free conditions)
CC {ECO:0000269|PubMed:11157951};
CC KM=2.5 mM for Li(+) (under K(+)-free conditions)
CC {ECO:0000269|PubMed:11157951};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. {ECO:0000305}.
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DR EMBL; BA000022; BAA17925.1; -; Genomic_DNA.
DR PIR; S75063; S75063.
DR AlphaFoldDB; P73863; -.
DR IntAct; P73863; 4.
DR STRING; 1148.1653008; -.
DR TCDB; 2.A.36.7.2; the monovalent cation:proton antiporter-1 (cpa1) family.
DR PaxDb; P73863; -.
DR EnsemblBacteria; BAA17925; BAA17925; BAA17925.
DR KEGG; syn:slr1727; -.
DR eggNOG; COG0025; Bacteria.
DR InParanoid; P73863; -.
DR OMA; AGMMMNY; -.
DR PhylomeDB; P73863; -.
DR SABIO-RK; P73863; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR GO; GO:0098719; P:sodium ion import across plasma membrane; IBA:GO_Central.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR InterPro; IPR004705; Cation/H_exchanger_CPA1_bac.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR TIGRFAMs; TIGR00831; a_cpa1; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell membrane; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..527
FT /note="Low-affinity Na(+)/H(+) antiporter NhaS1"
FT /id="PRO_0000423927"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 240..260
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..372
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 527 AA; 57539 MW; 499A0FEFACC4EB0E CRC64;
MDTAVNESLS ISYNLEQFLI VLSVSLSIAT LSKTVPILRK IPYTLLLVIV GMALAFVDVK
LINLSPELIM EIFLPPLLFE AAWNLQWRNL KENWFPITLF ATLGVVICVV GIAFPLSYWG
GMELAIAFLA AAALSATDPV SVIALFKELG ASKKLNTLME GESLFNDGVA VVVFLILVGI
PLGTSTFDLS VTLARFVTVI GIGVGCGLVI GFSLSLLTQR FDLPFVEQSL TLVSAYGAYI
LAENLGGSGV IGVVVVGMVL GNYGSRIGMN PRTRLIVSIF WEFVAFFVNS IIFLLIGDQI
GLSSLSDHLN LILIAIAAVV VTRLVSVFGL SLISNKVSDQ ISSTHITLQE QTVLWWGGLR
GSVAIAVALS VPQAIAERQA IIDIVFGVVL FTLLVQGLTT QFVLKGLDLI GDQPQRLEYA
ELVSRQIALR RVLAELEKTD EFPDINPERL RYKQELVQGQ LQSVTDKLKL LLQEYPLLQE
VANKKFDQTV LDIEAETYAD LIRMGRLEEN IMPLLVTLEG ENVAEPS
