NHAS2_SYNY3
ID NHAS2_SYNY3 Reviewed; 540 AA.
AC P74393;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Na(+)/H(+) antiporter NhaS2;
DE AltName: Full=Sodium/proton antiporter NhaS2;
GN Name=nhaS2; OrderedLocusNames=sll0273;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [2]
RP FUNCTION IN SODIUM UPTAKE, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX DOI=10.1046/j.1365-3040.2000.00565.x;
RA Mikkat S., Milkowski C., Hagemann M.;
RT "The gene sll0273 of the cyanobacterium Synechocystis sp. strain PCC6803
RT encodes a protein essential for growth at low Na+/K+ ratios.";
RL Plant Cell Environ. 23:549-559(2000).
RN [3]
RP GENE NAME.
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=11157951; DOI=10.1128/jb.183.4.1376-1384.2001;
RA Inaba M., Sakamoto A., Murata N.;
RT "Functional expression in Escherichia coli of low-affinity and high-
RT affinity Na(+)(Li(+))/H(+) antiporters of Synechocystis.";
RL J. Bacteriol. 183:1376-1384(2001).
CC -!- FUNCTION: Required for Na(+) uptake into the cell, especially at low
CC external Na(+) concentrations or low Na(+)/K(+) ratios. May be part of
CC a sodium cycle that permits re-entry of sodium into the cell.
CC {ECO:0000269|Ref.2}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Mutant cannot grow at low sodium concentrations
CC and exhibits an increased sensitivity towards potassium.
CC {ECO:0000269|Ref.2}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. {ECO:0000305}.
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DR EMBL; BA000022; BAA18490.1; -; Genomic_DNA.
DR PIR; S76231; S76231.
DR AlphaFoldDB; P74393; -.
DR SMR; P74393; -.
DR IntAct; P74393; 1.
DR STRING; 1148.1653577; -.
DR PaxDb; P74393; -.
DR EnsemblBacteria; BAA18490; BAA18490; BAA18490.
DR KEGG; syn:sll0273; -.
DR eggNOG; COG0025; Bacteria.
DR InParanoid; P74393; -.
DR OMA; TPPFWAS; -.
DR PhylomeDB; P74393; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR GO; GO:0098719; P:sodium ion import across plasma membrane; IBA:GO_Central.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell membrane; Ion transport; Membrane; Reference proteome;
KW Sodium; Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..540
FT /note="Na(+)/H(+) antiporter NhaS2"
FT /id="PRO_0000423928"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..343
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 540 AA; 58117 MW; 026BE9331EE244F9 CRC64;
MIKLPVLLAD INIQSLPTEP ELILNNLAIT TLVENLIILL LVATLVALVA RWLKIPYVIG
LVLAGLAIPR GLSVGLNPEL ILNFFLPILI FEAAINTDIS RLRSTIKPIT VLAGPGVVIS
AAITAVLLKI GLGLAWVTAA GVSVILTITD TVSVIAAFRS VPVPRRLATI VEGESMLNDG
VAMVLLSVIT TIHIQGGFSA GEGIRQIFVA FVGGGLVGLG LGYLCVGLFR QLNDDLSDIL
LTVSVSLGTF QIGQMLGVSS AIAVVVAGLV IGNLALKQTS ASIKVTLLSF WEYAGFGVNT
LIFLLVGIEV YPSILLSTIP AALIAIVAYQ IGRVFSIYPL LYLLSFFDRP LPLRWQHVLI
AGNVKGSLSM ALALALPLTL PGRDQVVTLV FSTVMVSLIG QGLSLPWVVK KLQLSKPSPL
AQKIAMLQLN LVTAKAAQGE LKYLLEAGSL PKFLYEELFA DYQARIANSE QELREFYNQR
NLIFSEGEVE KKYIDGLYRR LYIAEKSAIN DALAKGILAD DISDEYLQVL NEKLLALQDD
