NHAS3_SYNY3
ID NHAS3_SYNY3 Reviewed; 461 AA.
AC Q55190;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=High-affinity Na(+)/H(+) antiporter NhaS3;
DE AltName: Full=Sodium/proton antiporter NhaS3;
GN Name=nhaS3; OrderedLocusNames=sll0689;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [2]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND GENE NAME.
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=11157951; DOI=10.1128/jb.183.4.1376-1384.2001;
RA Inaba M., Sakamoto A., Murata N.;
RT "Functional expression in Escherichia coli of low-affinity and high-
RT affinity Na(+)(Li(+))/H(+) antiporters of Synechocystis.";
RL J. Bacteriol. 183:1376-1384(2001).
RN [3]
RP FUNCTION.
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=11996656; DOI=10.1023/a:1015281906254;
RA Elanskaya I.V., Karandashova I.V., Bogachev A.V., Hagemann M.;
RT "Functional analysis of the Na+/H+ antiporter encoding genes of the
RT cyanobacterium Synechocystis PCC 6803.";
RL Biochemistry (Mosc.) 67:432-440(2002).
RN [4]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION,
RP AND MUTAGENESIS OF ASP-99; GLU-114; GLU-121; ASP-217; ASP-218; GLU-359 AND
RP GLU-402.
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=19372598; DOI=10.1074/jbc.m109.001875;
RA Tsunekawa K., Shijuku T., Hayashimoto M., Kojima Y., Onai K., Morishita M.,
RA Ishiura M., Kuroda T., Nakamura T., Kobayashi H., Sato M., Toyooka K.,
RA Matsuoka K., Omata T., Uozumi N.;
RT "Identification and characterization of the Na+/H+ antiporter Nhas3 from
RT the thylakoid membrane of Synechocystis sp. PCC 6803.";
RL J. Biol. Chem. 284:16513-16521(2009).
CC -!- FUNCTION: Na(+)/H(+) antiporter that transports sodium from the
CC cytoplasm into the thylakoid lumen in exchange for protons. Contributes
CC to sodium homeostasis and tolerance. Has also Li(+)/H(+) antiport
CC activity under K(+)-free conditions, but not under K(+)-rich
CC conditions. {ECO:0000269|PubMed:11157951, ECO:0000269|PubMed:11996656,
CC ECO:0000269|PubMed:19372598}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.7 mM for Na(+) (under K(+)-free conditions)
CC {ECO:0000269|PubMed:11157951, ECO:0000269|PubMed:19372598};
CC KM=0.01 mM for Li(+) (under K(+)-free conditions)
CC {ECO:0000269|PubMed:11157951, ECO:0000269|PubMed:19372598};
CC pH dependence:
CC Activity is pH-independent. {ECO:0000269|PubMed:11157951,
CC ECO:0000269|PubMed:19372598};
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane
CC {ECO:0000269|PubMed:19372598}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:19372598}.
CC -!- INDUCTION: Induced in response to increased CO(2) concentrations.
CC Expression is under circadian control. {ECO:0000269|PubMed:19372598}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 2 (CPA2)
CC transporter (TC 2.A.37) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BA000022; BAA10332.1; -; Genomic_DNA.
DR PIR; S74414; S74414.
DR AlphaFoldDB; Q55190; -.
DR SMR; Q55190; -.
DR IntAct; Q55190; 1.
DR STRING; 1148.1001188; -.
DR TCDB; 2.A.37.2.4; the monovalent cation:proton antiporter-2 (cpa2) family.
DR PaxDb; Q55190; -.
DR EnsemblBacteria; BAA10332; BAA10332; BAA10332.
DR KEGG; syn:sll0689; -.
DR eggNOG; COG0475; Bacteria.
DR InParanoid; Q55190; -.
DR OMA; CAWATTA; -.
DR PhylomeDB; Q55190; -.
DR SABIO-RK; Q55190; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015299; F:solute:proton antiporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 1: Evidence at protein level;
KW Antiport; Ion transport; Membrane; Reference proteome; Sodium;
KW Sodium transport; Thylakoid; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..461
FT /note="High-affinity Na(+)/H(+) antiporter NhaS3"
FT /id="PRO_0000423929"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 99
FT /note="D->A: Lack of activity."
FT /evidence="ECO:0000269|PubMed:19372598"
FT MUTAGEN 114
FT /note="E->A: Strong decrease in activity."
FT /evidence="ECO:0000269|PubMed:19372598"
FT MUTAGEN 121
FT /note="E->Q: Lack of activity."
FT /evidence="ECO:0000269|PubMed:19372598"
FT MUTAGEN 217
FT /note="D->A,N: Lack of activity."
FT /evidence="ECO:0000269|PubMed:19372598"
FT MUTAGEN 218
FT /note="D->A,N: Lack of activity."
FT /evidence="ECO:0000269|PubMed:19372598"
FT MUTAGEN 359
FT /note="E->Q: Strong decrease in activity."
FT /evidence="ECO:0000269|PubMed:19372598"
FT MUTAGEN 402
FT /note="E->A,Q: Lack of activity."
FT /evidence="ECO:0000269|PubMed:19372598"
SQ SEQUENCE 461 AA; 47872 MW; 832E3E7BC17622F6 CRC64;
MFMNPLLPPL WPMIATAVET ETEIAPLVLA GVLLSLVVIY FASKLGGEVC LRLNLPPVLG
ELVGGVLVGV SALKLLLFPE GGLAPEDSLV IQLLMGSADL SPEAAQSVFS AQSEVISVIS
ELGVIILLFE IGLESNLKEL IRVGPQAAIV AVVGVVTPFS LGTIGLMTIF GVAAIPAIFA
GAALTATSIG ITAKVLAEIN RLSSNEGQII IGAAVLDDIL GIIVLAVVGS LVKTGEIQIS
NIIYLILSAT GFVVGSILIG RLLSPFYVSL VNRMKTRGQL LLVSICVAFV LSYIAQIVQL
EAILGSFAAG LILAETEKRE DLEEQILPLA DFFVPVFFVC VGAKTDVSVL NPAVPANREG
LIIAAFLILV AIVGKVVTGF TLFGKSELNK LAIGVGMIPR GEVGLVFAGV GAASGALDPA
TDAAIIVMVI VTTFVAPPWL RAVFEGAKKE EAPEKPVPTP D
