NHD1_ARATH
ID NHD1_ARATH Reviewed; 576 AA.
AC Q9LT68;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Sodium/proton antiporter 1 {ECO:0000303|PubMed:11500563};
DE Short=AtNHD1 {ECO:0000303|PubMed:11500563};
DE Short=Na(+)/H(+) antiporter 1 {ECO:0000303|PubMed:11500563};
DE Flags: Precursor;
GN Name=NHD1 {ECO:0000303|PubMed:11500563};
GN OrderedLocusNames=At3g19490 {ECO:0000312|Araport:AT3G19490};
GN ORFNames=MLD14.23 {ECO:0000312|EMBL:BAB02474.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP REVIEW, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=24617758; DOI=10.1111/tpj.12501;
RA Mueller M., Kunz H.H., Schroeder J.I., Kemp G., Young H.S., Neuhaus H.E.;
RT "Decreased capacity for sodium export out of Arabidopsis chloroplasts
RT impairs salt tolerance, photosynthesis and plant performance.";
RL Plant J. 78:646-658(2014).
CC -!- FUNCTION: Na(+)/H(+) antiporter that extrudes sodium in exchange for
CC external protons. {ECO:0000269|PubMed:24617758}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:24617758}; Multi-pass membrane protein
CC {ECO:0000255}. Plastid, chloroplast envelope
CC {ECO:0000269|PubMed:24617758}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in mature and senescent leaves,
CC and, to a lower extent, in seeds, roots, shoots, flowers and developing
CC siliques. {ECO:0000269|PubMed:24617758}.
CC -!- DISRUPTION PHENOTYPE: Decreased biomasses and lower chlorophyll levels
CC after sodium feeding. Increased Na(+) levels in chloroplasts in high
CC sodium chloride conditions but impaired photosynthetic performance.
CC Altered levels of phenylalanine and tyrosine.
CC {ECO:0000269|PubMed:24617758}.
CC -!- SIMILARITY: Belongs to the NhaD Na(+)/H(+) (TC 2.A.62) antiporter
CC family. {ECO:0000305}.
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DR EMBL; AB025624; BAB02474.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76247.1; -; Genomic_DNA.
DR EMBL; AY084922; AAM61484.1; -; mRNA.
DR RefSeq; NP_566638.1; NM_112836.4.
DR AlphaFoldDB; Q9LT68; -.
DR STRING; 3702.AT3G19490.1; -.
DR PaxDb; Q9LT68; -.
DR PRIDE; Q9LT68; -.
DR ProteomicsDB; 251134; -.
DR EnsemblPlants; AT3G19490.1; AT3G19490.1; AT3G19490.
DR GeneID; 821483; -.
DR Gramene; AT3G19490.1; AT3G19490.1; AT3G19490.
DR KEGG; ath:AT3G19490; -.
DR Araport; AT3G19490; -.
DR TAIR; locus:2090664; AT3G19490.
DR eggNOG; ENOG502QQAH; Eukaryota.
DR HOGENOM; CLU_029697_1_1_1; -.
DR InParanoid; Q9LT68; -.
DR OMA; DAHKGFS; -.
DR OrthoDB; 1052970at2759; -.
DR PhylomeDB; Q9LT68; -.
DR PRO; PR:Q9LT68; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LT68; baseline and differential.
DR GO; GO:0009941; C:chloroplast envelope; IDA:UniProtKB.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IDA:UniProtKB.
DR GO; GO:0050833; F:pyruvate transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0006848; P:pyruvate transport; IMP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IMP:UniProtKB.
DR GO; GO:0006814; P:sodium ion transport; IDA:UniProtKB.
DR InterPro; IPR004680; Cit_transptr-like_dom.
DR InterPro; IPR045016; NhaD-like.
DR PANTHER; PTHR43269; PTHR43269; 1.
DR Pfam; PF03600; CitMHS; 1.
PE 2: Evidence at transcript level;
KW Antiport; Chloroplast; Ion transport; Membrane; Plastid;
KW Reference proteome; Sodium; Sodium transport; Transit peptide;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..60
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN 61..576
FT /note="Sodium/proton antiporter 1"
FT /id="PRO_0000437697"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 462..482
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 501..521
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 541..561
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 576 AA; 61368 MW; 24C897C787F8A972 CRC64;
MAVFPIGSHF APPHQLTKRH VIATSSPISI STRLPQNVSF SKVSGVTGST RLSKHGVLVR
AEDKIRSSSS PSSLDEPIDE DLMDSSGLCD PLCSVDEPSS SYFEANYQPK TDIIKALAIL
AAALTGTAAI NHSWVAANQD VAMALLFGIG YAGIIFEESL AFNKSGIGLL MAVSLWVVRS
IGAPSTEIAV LDLQHATAEV SEIVFFLLGA MTIVEIVDAH QGFKLVTDNI TTRKPKTLLW
VVGFVTFFLS SILDNLTSTI VMVSLIRKLV PQSEYRKLLG GVVVIAANAG GAWTPIGDVT
TTMLWIHGQI STLPTMKDLF LPSVVSLAVP LALMSLTSEV NGKEQDPKDV LASEKMAPRG
KLVFGVGLGA LVFVPVFKAL TGLPPYMGIL LGLGVLWILT DAIHYGESER QKLKVPQALS
RIDTQGALFF LGILLSVSSL EAAGILREIA NYLDANIPNV ELIASAIGVV SAIIDNVPLV
AATMGMYDLT SFPQDSEFWQ LIAFCAGTGG SMLVIGSAAG VAFMGMEKVD FFWYFRKVSG
FAFAGYAAGI AAYLAVHNLH FEIPTTVAQI PFLTGS