NHEB_ONCMY
ID NHEB_ONCMY Reviewed; 759 AA.
AC Q01345;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Na(+)/H(+) exchanger beta;
DE AltName: Full=Beta-NHE;
DE AltName: Full=Na(+)/H(+) antiporter;
OS Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC Salmonidae; Salmoninae; Oncorhynchus.
OX NCBI_TaxID=8022;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Nucleated erythrocyte;
RX PubMed=1379718; DOI=10.1073/pnas.89.15.6765;
RA Borgese F., Sardet C., Cappadoro M., Pouyssegur J., Motais R.;
RT "Cloning and expression of a cAMP-activated Na+/H+ exchanger: evidence that
RT the cytoplasmic domain mediates hormonal regulation.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:6765-6769(1992).
CC -!- FUNCTION: Involved in pH regulation to eliminate acids generated by
CC active metabolism or to counter adverse environmental conditions. Major
CC proton extruding system driven by the inward sodium ion chemical
CC gradient.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane; Multi-pass membrane
CC protein.
CC -!- PTM: Activated by cAMP, protein kinase A and protein kinase C.
CC -!- MISCELLANEOUS: Inhibited by amiloride and 5-amino-substituted
CC derivatives and activated in a cooperative fashion by intracellular
CC H(+).
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. {ECO:0000305}.
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DR EMBL; M94581; AAA49549.1; -; mRNA.
DR PIR; A46188; A46188.
DR RefSeq; NP_001118167.1; NM_001124695.1.
DR AlphaFoldDB; Q01345; -.
DR SMR; Q01345; -.
DR GeneID; 100136740; -.
DR KEGG; omy:100136740; -.
DR CTD; 795135; -.
DR OrthoDB; 389547at2759; -.
DR GO; GO:0016323; C:basolateral plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IEA:InterPro.
DR GO; GO:0006885; P:regulation of pH; IEA:InterPro.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR InterPro; IPR004709; NaH_exchanger.
DR InterPro; IPR001970; NHE-1-like.
DR InterPro; IPR032103; NHE_CaM-bd.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR PANTHER; PTHR10110:SF59; PTHR10110:SF59; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR Pfam; PF16644; NEXCaM_BD; 1.
DR PRINTS; PR01084; NAHEXCHNGR.
DR PRINTS; PR01085; NAHEXCHNGR1.
DR TIGRFAMs; TIGR00840; b_cpa1; 1.
PE 2: Evidence at transcript level;
KW Antiport; Cell membrane; Glycoprotein; Ion transport; Membrane;
KW Phosphoprotein; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..759
FT /note="Na(+)/H(+) exchanger beta"
FT /id="PRO_0000052369"
FT TOPO_DOM 1..14
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 15..34
FT /note="Helical; Name=M1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..75
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..95
FT /note="Helical; Name=M2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 96..97
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..117
FT /note="Helical; Name=M3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 118..122
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..142
FT /note="Helical; Name=M4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 143..149
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..169
FT /note="Helical; Name=M5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..195
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..214
FT /note="Helical; Name=M5A"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..225
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..244
FT /note="Helical; Name=M5B"
FT /evidence="ECO:0000255"
FT TOPO_DOM 245..261
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical; Name=M6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 283..311
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..330
FT /note="Helical; Name=M7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 331..352
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..372
FT /note="Helical; Name=M8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 373..376
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..398
FT /note="Helical; Name=M9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..446
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 447..467
FT /note="Helical; Name=M10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 468..759
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 681..759
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 681..695
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 743..759
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 641
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000255"
FT MOD_RES 648
FT /note="Phosphoserine; by PKA"
FT /evidence="ECO:0000255"
FT CARBOHYD 49
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 338
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 759 AA; 85174 MW; D6D95442995AE251 CRC64;
MPAFSCAFPG CRRDLLVIVL VVFVGIGLPI EASAPAYQSH GTEGSHLTNI TNTKKAFPVL
AVNYEHVRKP FEIALWILLA LLMKLGFHLI PRLSAVVPES CLLIVVGLLV GGLIKVIGEE
PPVLDSQLFF LCLLPPIILD AGYFLPIRPF TENVGTILVF AVIGTLWNAF FMGGLLYALC
QIESVGLSGV DLLACLLFGS IVSAVDPVAV LAVFEEIHIN ELVHILVFGE SLLNDAVTVV
LYNLFEEFSK VGTVTVLDVF LGVVCFFVVS LGGVLVGAIY GFLAAFTSRF TSHTRVIEPL
FVFLYSYMAY LSSEMFHLSG IMALIACGVV MRPYVEANIS HKSYTTIKYF LKMWSSVSET
LIFIFLGVST VAGPHAWNWT FVITTVILCL VSRVLGVIGL TFIINKFRIV KLTKKDQFIV
AYGGLRGAIA FSLGYLLSNS HQMRNLFLTA IITVIFFTVF VQGMTIRPLV ELLAVKKKKE
SKPSINEEIH TEFLDHLLTG VEGVCGHYGH YHWKEKLNRF NKTYVKRWLI AGENFKEPEL
IAFYRKMELK QAIMMVESGQ LPSVLPSTIS MQNIQPRAIP RVSKKREEEI RRILRANLQN
NKQKMRSRSY SRHTLFDADE EDNVSEVRLR KTKMEMERRV SVMERRMSHY LTVPANRESP
RPGVRRVRFE SDNQVFSADS FPTVHFEQPS PPSTPDAVSL EEEEEEVPKR PSLKADIEGP
RGNASDNHQG ELDYQRLARC LSDPGPNKDK EDDDPFMSC
