ID   NHEJ1_DANRE             Reviewed;         309 AA.
AC   Q6NV18;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Non-homologous end-joining factor 1;
DE   AltName: Full=Protein cernunnos;
DE   AltName: Full=XRCC4-like factor;
GN   Name=nhej1; Synonyms=xlf; ORFNames=zgc:85657;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA repair protein involved in DNA non-homologous end joining
CC       (NHEJ); required for double-strand break (DSB) repair and V(D)J
CC       recombination. Plays a key role in NHEJ by promoting the ligation of
CC       various mismatched and non-cohesive ends. Associates with xrcc4 to form
CC       alternating helical filaments that bridge DNA and act like a bandage,
CC       holding together the broken DNA until it is repaired. The xrcc4-
CC       nhej1/xlf subcomplex binds to the DNA fragments of a DSB in a highly
CC       diffusive manner and robustly bridges two independent DNA molecules,
CC       holding the broken DNA fragments in close proximity to one other. The
CC       mobility of the bridges ensures that the ends remain accessible for
CC       further processing by other repair factors.
CC       {ECO:0000250|UniProtKB:Q9H9Q4}.
CC   -!- SUBUNIT: Homodimer. Interacts with xrcc4; the interaction is direct and
CC       is mediated via a head-to-head interaction between N-terminal head
CC       regions. Associates with the non-homologous end joining (NHEJ) complex
CC       which is at least composed of the core proteins xrcc5/Ku80, xrcc6/Ku70,
CC       prkdc/DNA-PKcs, nhej1/xlf, lig4 and xrcc4.
CC       {ECO:0000250|UniProtKB:Q9H9Q4}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9H9Q4}.
CC       Chromosome {ECO:0000250|UniProtKB:Q9H9Q4}. Note=Localizes to site of
CC       double-strand breaks. {ECO:0000250|UniProtKB:Q9H9Q4}.
CC   -!- DOMAIN: The coiled-coil region mediates homodimerization.
CC       {ECO:0000250|UniProtKB:Q9H9Q4}.
CC   -!- DOMAIN: The Leu-lock (Leu-114) site inserts into a hydrophobic pocket
CC       in xrcc4. {ECO:0000250|UniProtKB:Q9H9Q4}.
CC   -!- SIMILARITY: Belongs to the XRCC4-XLF family. XLF subfamily.
CC       {ECO:0000305}.
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DR   EMBL; BC068348; AAH68348.1; -; mRNA.
DR   RefSeq; NP_999914.1; NM_214749.1.
DR   AlphaFoldDB; Q6NV18; -.
DR   SMR; Q6NV18; -.
DR   STRING; 7955.ENSDARP00000124126; -.
DR   PaxDb; Q6NV18; -.
DR   PRIDE; Q6NV18; -.
DR   GeneID; 406593; -.
DR   KEGG; dre:406593; -.
DR   CTD; 79840; -.
DR   ZFIN; ZDB-GENE-040426-2515; nhej1.
DR   eggNOG; ENOG502QWAX; Eukaryota.
DR   InParanoid; Q6NV18; -.
DR   OrthoDB; 1397591at2759; -.
DR   PhylomeDB; Q6NV18; -.
DR   Reactome; R-DRE-5693571; Nonhomologous End-Joining (NHEJ).
DR   PRO; PR:Q6NV18; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0032807; C:DNA ligase IV complex; IBA:GO_Central.
DR   GO; GO:0070419; C:nonhomologous end joining complex; ISS:UniProtKB.
DR   GO; GO:0035861; C:site of double-strand break; ISS:UniProtKB.
DR   GO; GO:0045027; F:DNA end binding; ISS:UniProtKB.
DR   GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; ISS:UniProtKB.
DR   GO; GO:0033152; P:immunoglobulin V(D)J recombination; ISS:UniProtKB.
DR   Gene3D; 2.170.210.10; -; 1.
DR   InterPro; IPR015381; XLF_N.
DR   InterPro; IPR038051; XRCC4-like_N_sf.
DR   Pfam; PF09302; XLF; 1.
PE   2: Evidence at transcript level;
KW   Chromosome; DNA damage; DNA repair; DNA-binding; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..309
FT                   /note="Non-homologous end-joining factor 1"
FT                   /id="PRO_0000228657"
FT   REGION          223..309
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           299..309
FT                   /note="XLM"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H9Q4"
FT   COMPBIAS        251..293
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            114
FT                   /note="Leu-lock"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H9Q4"
SQ   SEQUENCE   309 AA;  34182 MW;  BB820FB2DBD6784B CRC64;
     MEAVLSALPW VPVNISGSDL LAKAWFGDSQ YRVLLTDLST VWEEEMSTDD IQSRAQDLNK
     RLRAPAQAFF SHLCSVARPC FSGLDEDQIS AAQAALEQHG ESLTVKLKSE LAGLPFYWEF
     RCTTTPVAVV CRQLVRPLLA MTLVLQRQAE DLAALLARKD AEIQDYQENG AVLSRARLQT
     EPFEVHQYKE NFITQILPQM NVTLDSLGFD SELQALYMAV NSGKTGRKRK HSPDSSPAAQ
     ENHITDHQHI SESTDVGPSL ASQEHNNAKE SGRSQVANSQ QTLPLSSTAG SEDRSTSRAK
     KKKAVGLFR