NHG1_PSEPU
ID NHG1_PSEPU Reviewed; 434 AA.
AC P23262;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Salicylate hydroxylase;
DE EC=1.14.13.1;
DE AltName: Full=Salicylate 1-monooxygenase;
GN Name=nahG;
OS Pseudomonas putida (Arthrobacter siderocapsulatus).
OG Plasmid NAH7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-26.
RC STRAIN=ATCC 17485 / DSM 50208 / JCM 6158 / NCIMB 12092 / Stanier 111 /
RC Biotype A;
RX PubMed=1993181; DOI=10.1021/bi00220a028;
RA You I.-S., Ghosal D., Gunsalus I.C.;
RT "Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate
RT hydroxylase gene (nahG) and its 3'-flanking region.";
RL Biochemistry 30:1635-1641(1991).
RN [2]
RP PROTEIN SEQUENCE OF 2-26, AND SUBUNIT.
RX PubMed=2363715; DOI=10.1016/0006-291x(90)92000-p;
RA You I.-S., Murray R.I., Jollie D., Gunsalus I.C.;
RT "Purification and characterization of salicylate hydroxylase from
RT Pseudomonas putida PpG7.";
RL Biochem. Biophys. Res. Commun. 169:1049-1054(1990).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + NADH + O2 + salicylate = catechol + CO2 + H2O +
CC NAD(+); Xref=Rhea:RHEA:11004, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:18135,
CC ChEBI:CHEBI:30762, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC EC=1.14.13.1;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:2363715}.
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DR EMBL; M60055; AAA25897.1; -; Genomic_DNA.
DR PIR; A39181; A39181.
DR RefSeq; WP_011475386.1; NC_007926.1.
DR RefSeq; YP_534831.1; NC_007926.1.
DR PDB; 6BZ5; X-ray; 2.01 A; A/B=1-434.
DR PDBsum; 6BZ5; -.
DR AlphaFoldDB; P23262; -.
DR SMR; P23262; -.
DR KEGG; ag:AAA25897; -.
DR BioCyc; MetaCyc:MON-12811; -.
DR UniPathway; UPA00082; -.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0018658; F:salicylate 1-monooxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR002938; FAD-bd.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR017631; Salicylate_mOase.
DR Pfam; PF01494; FAD_binding_3; 2.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR03219; salicylate_mono; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aromatic hydrocarbons catabolism; Direct protein sequencing;
KW FAD; Flavoprotein; Monooxygenase; NAD; Oxidoreductase; Plasmid.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:1993181,
FT ECO:0000269|PubMed:2363715"
FT CHAIN 2..434
FT /note="Salicylate hydroxylase"
FT /id="PRO_0000096803"
FT BINDING 9..38
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
FT STRAND 8..13
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 17..26
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 32..37
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 44..46
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 54..62
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 66..72
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 83..89
FT /evidence="ECO:0007829|PDB:6BZ5"
FT TURN 90..92
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 95..100
FT /evidence="ECO:0007829|PDB:6BZ5"
FT TURN 102..104
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 111..120
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 133..138
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 143..147
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 152..160
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 168..175
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 184..196
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 197..206
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 212..215
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 218..222
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 225..232
FT /evidence="ECO:0007829|PDB:6BZ5"
FT TURN 233..236
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 237..245
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 260..263
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 266..272
FT /evidence="ECO:0007829|PDB:6BZ5"
FT TURN 273..275
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 278..285
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 291..297
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 304..306
FT /evidence="ECO:0007829|PDB:6BZ5"
FT STRAND 309..311
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 314..317
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 321..323
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 326..341
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 351..361
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 363..380
FT /evidence="ECO:0007829|PDB:6BZ5"
FT TURN 385..389
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 391..400
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 403..406
FT /evidence="ECO:0007829|PDB:6BZ5"
FT HELIX 410..421
FT /evidence="ECO:0007829|PDB:6BZ5"
SQ SEQUENCE 434 AA; 46964 MW; 78EE1AD569283590 CRC64;
MKNNKLGLRI GIVGGGISGV ALALELCRYS HIQVQLFEAA PAFGEVGAGV SFGPNAVRAI
VGLGLGEAYL QVADRTSEPW EDVWFEWRRG SDASYLGATI APGVGQSSVH RADFIDALVT
HLPEGIAQFG KRATQVEQQG GEVQVLFTDG TEYRCDLLIG ADGIKSALRS HVLEGQGLAP
QVPRFSGTCA YRGMVDSLHL REAYRAHGID EHLVDVPQMY LGLDGHILTF PVRNGGIINV
VAFISDRSEP KPTWPADAPW VREASQREML DAFAGWGDAA RALLECIPAP TLWALHDLAE
LPGYVHGRVV LIGDAAHAML PHQGAGAGQG LEDAYFLARL LGDTQADAGN LAELLEAYDD
LRRPRACRVQ QTSWETGELY ELRDPVVGAN EQLLGENLAT RFDWLWNHDL DTDLAEARAR
LGWEHGGGGA LRQG
