NHG2_PSEPU
ID NHG2_PSEPU Reviewed; 435 AA.
AC Q53552;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Salicylate hydroxylase;
DE EC=1.14.13.1;
DE AltName: Full=Salicylate 1-monooxygenase;
GN Name=nahG;
OS Pseudomonas putida (Arthrobacter siderocapsulatus).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=303;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=KF715;
RX PubMed=8561793; DOI=10.1006/bbrc.1996.0097;
RA Lee J., Oh J., Min K.R., Kim Y.;
RT "Nucleotide sequence of salicylate hydroxylase gene and its 5'-flanking
RT region of Pseudomonas putida KF715.";
RL Biochem. Biophys. Res. Commun. 218:544-548(1996).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + NADH + O2 + salicylate = catechol + CO2 + H2O +
CC NAD(+); Xref=Rhea:RHEA:11004, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16526, ChEBI:CHEBI:18135,
CC ChEBI:CHEBI:30762, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC EC=1.14.13.1;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
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DR EMBL; S80995; AAB35960.1; -; Genomic_DNA.
DR PIR; JC4590; JC4590.
DR AlphaFoldDB; Q53552; -.
DR SMR; Q53552; -.
DR UniPathway; UPA00082; -.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0018658; F:salicylate 1-monooxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; -; 1.
DR InterPro; IPR002938; FAD-bd.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR017631; Salicylate_mOase.
DR Pfam; PF01494; FAD_binding_3; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR03219; salicylate_mono; 1.
PE 4: Predicted;
KW Aromatic hydrocarbons catabolism; FAD; Flavoprotein; Monooxygenase; NAD;
KW Oxidoreductase; Plasmid.
FT CHAIN 1..435
FT /note="Salicylate hydroxylase"
FT /id="PRO_0000096804"
FT BINDING 12..41
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255"
SQ SEQUENCE 435 AA; 47914 MW; 7E8017520726DD8C CRC64;
MNDMNAKKPA LRVAIVGGGI SGLALALSLC KHSHLNVQLF EAAPAFGEVG AGVSFGPNAV
RAIVGLGLGQ AYFQVADRTP QPWEDIWFEW RRGSDASYLG ATIAGVGQSS VHRADFLDAL
VKHLPEGIAQ FRKRATQIEQ QGDELQVLFR DGTEYRCDLL IGRDGIKSAL RSYVLEGQGQ
DHLEPRFSGT CAYRGMVDSL QLREAYRING IDEHLVDVPQ MYLGLYGHIL TFPVRKGRIV
NVVAFTSDRS QPEPTWPADA PWVREASQRE MLDAFAGWGD ARALLECIPA PTLWALHDLP
ELPGYVHGRV ALIGDAAHAM LPHQGAGAGQ GLEDAYFLAR LLGDSRTETG NLPELLGAYD
DLRRPHACRV QRTTVETGEL YELRDPIVGA DEQLVGEILA TRFDWLWNHD LDADVAEARL
RMGWEAHEQI ALRQG