NHL1_CAEEL
ID NHL1_CAEEL Reviewed; 974 AA.
AC Q03601; Q23510;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=RING finger protein nhl-1;
GN Name=nhl-1 {ECO:0000312|WormBase:F54G8.4a};
GN ORFNames=F54G8.4 {ECO:0000312|WormBase:F54G8.4a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP INTERACTION WITH UBC-13.
RX PubMed=15530417; DOI=10.1016/j.bbrc.2004.10.047;
RA Gudgen M., Chandrasekaran A., Frazier T., Boyd L.;
RT "Interactions within the ubiquitin pathway of Caenorhabditis elegans.";
RL Biochem. Biophys. Res. Commun. 325:479-486(2004).
RN [4]
RP DISRUPTION PHENOTYPE.
RX PubMed=18652816; DOI=10.1016/j.ydbio.2008.06.037;
RA Hyenne V., Desrosiers M., Labbe J.C.;
RT "C. elegans Brat homologs regulate PAR protein-dependent polarity and
RT asymmetric cell division.";
RL Dev. Biol. 321:368-378(2008).
CC -!- SUBUNIT: Interacts with ubc-13. {ECO:0000269|PubMed:15530417}.
CC -!- INTERACTION:
CC Q03601; G5EFS1: ikb-1; NbExp=5; IntAct=EBI-314158, EBI-314119;
CC Q03601; G5EC32: sorb-1; NbExp=7; IntAct=EBI-314158, EBI-325337;
CC -!- DISRUPTION PHENOTYPE: Double knockout with par-2 mutant (it5ts)
CC partially suppresses the lethality phenotype of the par-2 mutant
CC (it5ts) at 25 degrees Celsius. {ECO:0000269|PubMed:18652816}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX284603; CAA79562.1; -; Genomic_DNA.
DR EMBL; Z22177; CAA79562.1; JOINED; Genomic_DNA.
DR PIR; E88549; E88549.
DR PIR; S28275; S28275.
DR RefSeq; NP_499028.1; NM_066627.5.
DR AlphaFoldDB; Q03601; -.
DR SMR; Q03601; -.
DR BioGRID; 41494; 20.
DR DIP; DIP-26359N; -.
DR IntAct; Q03601; 14.
DR STRING; 6239.F54G8.4; -.
DR iPTMnet; Q03601; -.
DR EPD; Q03601; -.
DR PaxDb; Q03601; -.
DR PeptideAtlas; Q03601; -.
DR PRIDE; Q03601; -.
DR EnsemblMetazoa; F54G8.4a.1; F54G8.4a.1; WBGene00003597.
DR GeneID; 176295; -.
DR UCSC; F54G8.4; c. elegans.
DR CTD; 176295; -.
DR WormBase; F54G8.4a; CE05942; WBGene00003597; nhl-1.
DR eggNOG; KOG2177; Eukaryota.
DR GeneTree; ENSGT00940000168201; -.
DR HOGENOM; CLU_002505_1_0_1; -.
DR InParanoid; Q03601; -.
DR OMA; SEPGCFT; -.
DR OrthoDB; 489543at2759; -.
DR PhylomeDB; Q03601; -.
DR SignaLink; Q03601; -.
DR PRO; PR:Q03601; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00003597; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR ExpressionAtlas; Q03601; baseline and differential.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030371; F:translation repressor activity; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:WormBase.
DR GO; GO:0017148; P:negative regulation of translation; IBA:GO_Central.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IDA:WormBase.
DR Gene3D; 2.120.10.30; -; 3.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR001258; NHL_repeat.
DR InterPro; IPR018957; Znf_C3HC4_RING-type.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR Pfam; PF01436; NHL; 5.
DR Pfam; PF00097; zf-C3HC4; 1.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS51125; NHL; 6.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Metal-binding; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..974
FT /note="RING finger protein nhl-1"
FT /id="PRO_0000055983"
FT REPEAT 699..742
FT /note="NHL 1"
FT REPEAT 746..788
FT /note="NHL 2"
FT REPEAT 792..835
FT /note="NHL 3"
FT REPEAT 839..883
FT /note="NHL 4"
FT REPEAT 887..930
FT /note="NHL 5"
FT REPEAT 934..974
FT /note="NHL 6"
FT ZN_FING 43..84
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..395
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 416..548
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 416..435
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 444..464
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 512..527
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 974 AA; 109138 MW; A4D195F9AA8BBF72 CRC64;
MSSSPQNEAE AREKMRELMS RPPSSRPADF VNPLEKIEQL LTCPICLDRY KQPKLLPCQH
TFCYPCLESC ADTLHRNLKC PECRAEHNIP YDGVKAFQPN YTLTGFLEIH LQATPESAAE
IEEYIHRYNL ERCKICDEKA DCEPCAHCDR KACKECRQTH MDMLKRDMSR LLNQVKRLAN
RITEASDNLS KGVDMMTMNC ETTKAEIKEY FHRYQRDLKK KEDNFLMEVD TFQATETRNM
SNLRDVLEIE SSNMSEAVSR LDAAIKGECS IEDSELVRMK NTFTEGLEYL RNFQPDADEL
FNRKLRFSAG DDAAKLPAAI TTSGELCVLV PQFSGRYLPL EQSYLPRPFR LPLESDSYRV
KSDERASMRD READRTSSRH SHRNPEPDES SIRYRRRQQL EDEAWNRLRN SSAAPSLLTT
SVTADSSSRT SPWAADRVTR SVEPTKSRPT SLIVPNTETP RTVSPASKPP LPPQSVERVE
RTEDASPAPL PQLPIRKPPL PRQQSSNDDS LNEKVETIRR AHQQRQDASR AASRAVSSEE
SEGEDFPVST NRGRIRIVCR AASVNRDDGL MSMIPGTGTI INVPPPQHQL PASAPITNGT
SEQVAIPVTH FTGEEDESDI VVPAWLQRRR QRFQRSRTNP DIQAQFTSAR VQQLLAERQS
RLDSSTTTDE EKEKLAVLRQ RGRSASREAG EWRARGRPRA VFGRKGAKDG ELNWPRGICA
LSGGLVATCD SSNHRVCVFD KDGKFVRQFG GYGAGAGQLD SAAGLASSKL RVIVSDRYNH
RISVFGLEGD HLFSFGGHGQ GNAKFNNPWG VAVDDLGSIY VADKDNHRVQ VFDKNGQFIA
KFGSFGHLPG QLNSPLFIAV SRVTHHVYVS DSSNHRISVF DPHGVHLFSF GEEGFHGGQF
KFPRGIAIDS QENLIIADSG NNRIQVFDAQ GQFVSSFGTW GGGAGQLKGV EDVCVTADGS
IVVTDRENHR IQIF
