NHL26_ARATH
ID NHL26_ARATH Reviewed; 213 AA.
AC Q9FI03;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=NDR1/HIN1-like protein 26 {ECO:0000303|Ref.4};
GN Name=NHL26 {ECO:0000303|Ref.4};
GN OrderedLocusNames=At5g53730 {ECO:0000312|Araport:AT5G53730};
GN ORFNames=MGN6.8 {ECO:0000312|EMBL:BAB09545.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1016/S0981-9428(00)01186-4;
RA Doermann P., Gopalan S., He S.Y., Benning C.;
RT "A gene family in Arabidopsis thaliana with sequence similarity to NDR1 and
RT HIN1.";
RL Plant Physiol. Biochem. 38:789-796(2000).
RN [5]
RP INDUCTION BY SENESCENCE.
RX PubMed=14617064; DOI=10.1046/j.1365-313x.2003.01908.x;
RA Gepstein S., Sabehi G., Carp M.-J., Hajouj T., Nesher M.F.O., Yariv I.,
RA Dor C., Bassani M.;
RT "Large-scale identification of leaf senescence-associated genes.";
RL Plant J. 36:629-642(2003).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=23715470; DOI=10.1105/tpc.113.111849;
RA Vilaine F., Kerchev P., Clement G., Batailler B., Cayla T., Bill L.,
RA Gissot L., Dinant S.;
RT "Increased expression of a phloem membrane protein encoded by NHL26 alters
RT phloem export and sugar partitioning in Arabidopsis.";
RL Plant Cell 25:1689-1708(2013).
CC -!- FUNCTION: Involved in the regulation of sugar, amino acid and some
CC primary metabolite export from companion cells (CCs) to sieve elements
CC (SEs) in phloem. Required for apoplastic phloem sugar loading in source
CC leaves in order to transport it to sink tissues. Required for correct
CC sugar partitioning between source leaves and sink organs.
CC {ECO:0000269|PubMed:23715470}.
CC -!- SUBCELLULAR LOCATION: Cell junction, plasmodesma
CC {ECO:0000269|PubMed:23715470}. Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:23715470}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the vasculature of roots, rosette
CC leaves, stems, cauline leaves and flowers. Specifically expressed in
CC phloem. {ECO:0000269|PubMed:23715470}.
CC -!- INDUCTION: Induced by senescence (PubMed:14617064). Down-regulated by
CC sucrose, glucose and fructose (PubMed:23715470).
CC {ECO:0000269|PubMed:14617064, ECO:0000269|PubMed:23715470}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:23715470}.
CC -!- MISCELLANEOUS: Plants over-expressing NHL26 exhibit slow growth,
CC accumulate high levels of carbohydrates in mature leaves, have
CC increased shoot biomass, decreased root biomass and decreased seed
CC yield. {ECO:0000269|PubMed:23715470}.
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DR EMBL; AB017066; BAB09545.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96399.1; -; Genomic_DNA.
DR EMBL; BT010854; AAR24221.1; -; mRNA.
DR EMBL; BT011320; AAR92356.1; -; mRNA.
DR RefSeq; NP_200184.1; NM_124752.4.
DR AlphaFoldDB; Q9FI03; -.
DR STRING; 3702.AT5G53730.1; -.
DR iPTMnet; Q9FI03; -.
DR PaxDb; Q9FI03; -.
DR PRIDE; Q9FI03; -.
DR EnsemblPlants; AT5G53730.1; AT5G53730.1; AT5G53730.
DR GeneID; 835454; -.
DR Gramene; AT5G53730.1; AT5G53730.1; AT5G53730.
DR KEGG; ath:AT5G53730; -.
DR Araport; AT5G53730; -.
DR TAIR; locus:2164092; AT5G53730.
DR eggNOG; ENOG502QTGM; Eukaryota.
DR HOGENOM; CLU_051752_1_1_1; -.
DR InParanoid; Q9FI03; -.
DR OMA; RWKIGTW; -.
DR OrthoDB; 1307089at2759; -.
DR PhylomeDB; Q9FI03; -.
DR PRO; PR:Q9FI03; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FI03; baseline and differential.
DR GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009506; C:plasmodesma; IBA:GO_Central.
DR GO; GO:0009511; C:plasmodesmatal endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0098542; P:defense response to other organism; IEA:InterPro.
DR GO; GO:0110126; P:phloem loading; IMP:TAIR.
DR InterPro; IPR004864; LEA_2.
DR InterPro; IPR044839; NDR1-like.
DR PANTHER; PTHR31415; PTHR31415; 1.
DR Pfam; PF03168; LEA_2; 1.
PE 2: Evidence at transcript level;
KW Cell junction; Endoplasmic reticulum; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..213
FT /note="NDR1/HIN1-like protein 26"
FT /id="PRO_0000442223"
FT TOPO_DOM 1..27
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 49..213
FT /note="Lumenal"
FT /evidence="ECO:0000305"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 77
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 213 AA; 23911 MW; CAC699DFDD09CF65 CRC64;
MSQISITSPK HCAKKGGINI NNRHKKLFFT FSTFFSGLLL IIFLVWLILH PERPEFSLTE
ADIYSLNLTT SSTHLLNSSV QLTLFSKNPN KKVGIYYDKL LVYAAYRGQQ ITSEASLPPF
YQSHEEINLL TAFLQGTELP VAQSFGYQIS RERSTGKIII GMKMDGKLRW KIGTWVSGAY
RFNVNCLAIV AFGMNMTTPP LASLQGTRCS TTI
