NHL3_ARATH
ID NHL3_ARATH Reviewed; 231 AA.
AC Q9FNH6; B0ZVP9; Q94K35; Q9LKQ2;
DT 17-FEB-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=NDR1/HIN1-like protein 3 {ECO:0000303|Ref.1};
GN Name=NHL3 {ECO:0000303|Ref.1};
GN OrderedLocusNames=At5g06320 {ECO:0000312|Araport:AT5G06320};
GN ORFNames=MHF15.16 {ECO:0000312|EMBL:BAB08954.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INDUCTION BY PSEUDOMONAS SYRINGAE, GENE FAMILY,
RP AND NOMENCLATURE.
RC STRAIN=cv. Col-2;
RX DOI=10.1016/S0981-9428(00)01186-4;
RA Doermann P., Gopalan S., He S.Y., Benning C.;
RT "A gene family in Arabidopsis thaliana with sequence similarity to NDR1 and
RT HIN1.";
RL Plant Physiol. Biochem. 38:789-796(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Stracke R., Palme K.;
RT "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves
RT and guard cells.";
RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 39-210.
RC STRAIN=cv. Ag-0, cv. Bay-0, cv. Bil-5, cv. Bil-7, cv. Bor-1, cv. Bor-4,
RC cv. Br-0, cv. C24, cv. Columbia, cv. Ct-1, cv. Cvi-0, cv. Ed-1, cv. Ed-2,
RC cv. Edi-0, cv. Est-1, cv. Fab-2, cv. Fab-4, cv. Fei-0, cv. Ga-0, cv. Gy-0,
RC cv. HR-10, cv. HR-5, cv. Kas-2, cv. KNO-10, cv. Kon, cv. KZ-1, cv. KZ-9,
RC cv. Ler-1, cv. Ll-0, cv. Lov-1, cv. Lov-5, cv. Lp2-2, cv. Lp2-6, cv. Mr-0,
RC cv. Mrk-0, cv. Mt-0, cv. Mz-0, cv. N13 Konchezero, cv. Nd-1, cv. NFA-10,
RC cv. NFA-8, cv. Omo2-1, cv. Omo2-3, cv. Pna-10, cv. Pro-0, cv. Pu2-7,
RC cv. Ra-0, cv. Rmx-A180, cv. RRS-10, cv. Se-0, cv. Sha, cv. Sorbo,
RC cv. Spr1-2, cv. Sq-8, cv. Tamm-2, cv. Tamm-27, cv. Ts-1, cv. Ts-5,
RC cv. Ull2-5, cv. Uod-1, cv. Uod-7, cv. Var2-1, cv. Var2-6, cv. Wa-1,
RC cv. Wassilewskija, cv. Wassilewskija-2, cv. Wei-0, cv. Wt-5, cv. Yo-0,
RC cv. Zdr-1, and cv. Zdr-6;
RX PubMed=18245336; DOI=10.1534/genetics.107.083279;
RA Bakker E.G., Traw M.B., Toomajian C., Kreitman M., Bergelson J.;
RT "Low levels of polymorphism in genes that control the activation of defense
RT response in Arabidopsis thaliana.";
RL Genetics 178:2031-2043(2008).
RN [7]
RP INDUCTION BY PSEUDOMONAS SYRINGAE; WOUNDING AND SALICYLIC ACID.
RC STRAIN=cv. Columbia;
RX PubMed=12059109; DOI=10.1094/mpmi.2002.15.6.608;
RA Varet A., Parker J., Tornero P., Nass N., Nuernberger T., Dangl J.L.,
RA Scheel D., Lee J.;
RT "NHL25 and NHL3, two NDR1/HIN1-1ike genes in Arabidopsis thaliana with
RT potential role(s) in plant defense.";
RL Mol. Plant Microbe Interact. 15:608-616(2002).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, AND SUBUNIT.
RC STRAIN=cv. Columbia;
RX PubMed=12913158; DOI=10.1104/pp.103.020438;
RA Varet A., Hause B., Hause G., Scheel D., Lee J.;
RT "The Arabidopsis NHL3 gene encodes a plasma membrane protein and its
RT overexpression correlates with increased resistance to Pseudomonas syringae
RT pv. tomato DC3000.";
RL Plant Physiol. 132:2023-2033(2003).
RN [9]
RP INDUCTION BY SPERMINE AND CUCUMBER MOSAIC VIRUS, AND TISSUE SPECIFICITY.
RC STRAIN=cv. C24;
RX PubMed=14666423; DOI=10.1007/s00425-003-1169-2;
RA Zheng M.S., Takahashi H., Miyazaki A., Hamamoto H., Shah J., Yamaguchi I.,
RA Kusano T.;
RT "Up-regulation of Arabidopsis thaliana NHL10 in the hypersensitive response
RT to Cucumber mosaic virus infection and in senescing leaves is controlled by
RT signalling pathways that differ in salicylate involvement.";
RL Planta 218:740-750(2004).
CC -!- FUNCTION: Confers resistance to Pseudomonas syringae pv. tomato DC3000
CC (Pst DC3000). {ECO:0000269|PubMed:12913158}.
CC -!- SUBUNIT: May form oligomers or be a component of larger protein complex
CC in plasma membranes. {ECO:0000269|PubMed:12913158}.
CC -!- INTERACTION:
CC Q9FNH6; Q5ICL9: NPR4; NbExp=3; IntAct=EBI-4461284, EBI-1392093;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12913158};
CC Single-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, young and senescing leaves,
CC cauline leaves, stems and siliques. {ECO:0000269|PubMed:14666423}.
CC -!- INDUCTION: Accumulates upon infection with avirulent Pseudomonas
CC syringae pv. tomato DC3000 (Pst DC3000) carrying avirulence genes
CC avrRpm1, avrRpt2, avrB, or avrRps4 but not with the virulent Pst DC3000
CC (Ref.1, PubMed:12059109). Induced by salicylic acid (SA). Accumulates
CC rapidly in local and systemic tissues after wounding (PubMed:12059109).
CC Triggered by spermine, an inducer of pathogenesis-related (PR) genes.
CC Up-regulated by cucumber mosaic virus (CMV-Y and CMV-B2 strains)
CC (PubMed:14666423). {ECO:0000269|PubMed:12059109,
CC ECO:0000269|PubMed:14666423, ECO:0000269|Ref.1}.
CC -!- PTM: Glycosylated. {ECO:0000269|PubMed:12913158}.
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DR EMBL; AF264699; AAF88023.1; -; mRNA.
DR EMBL; AF083702; AAN60261.1; -; mRNA.
DR EMBL; AB006700; BAB08954.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91002.1; -; Genomic_DNA.
DR EMBL; AF370332; AAK44147.2; -; mRNA.
DR EMBL; BT000871; AAN41271.1; -; mRNA.
DR EMBL; EU405623; ABZ03284.1; -; Genomic_DNA.
DR EMBL; EU405624; ABZ03285.1; -; Genomic_DNA.
DR EMBL; EU405627; ABZ03288.1; -; Genomic_DNA.
DR EMBL; EU405629; ABZ03290.1; -; Genomic_DNA.
DR EMBL; EU405630; ABZ03291.1; -; Genomic_DNA.
DR EMBL; EU405631; ABZ03292.1; -; Genomic_DNA.
DR EMBL; EU405632; ABZ03293.1; -; Genomic_DNA.
DR EMBL; EU405633; ABZ03294.1; -; Genomic_DNA.
DR EMBL; EU405634; ABZ03295.1; -; Genomic_DNA.
DR EMBL; EU405635; ABZ03296.1; -; Genomic_DNA.
DR EMBL; EU405636; ABZ03297.1; -; Genomic_DNA.
DR EMBL; EU405637; ABZ03298.1; -; Genomic_DNA.
DR EMBL; EU405638; ABZ03299.1; -; Genomic_DNA.
DR EMBL; EU405639; ABZ03300.1; -; Genomic_DNA.
DR EMBL; EU405640; ABZ03301.1; -; Genomic_DNA.
DR EMBL; EU405642; ABZ03303.1; -; Genomic_DNA.
DR EMBL; EU405643; ABZ03304.1; -; Genomic_DNA.
DR EMBL; EU405644; ABZ03305.1; -; Genomic_DNA.
DR EMBL; EU405646; ABZ03307.1; -; Genomic_DNA.
DR EMBL; EU405647; ABZ03308.1; -; Genomic_DNA.
DR EMBL; EU405648; ABZ03309.1; -; Genomic_DNA.
DR EMBL; EU405649; ABZ03310.1; -; Genomic_DNA.
DR EMBL; EU405650; ABZ03311.1; -; Genomic_DNA.
DR EMBL; EU405652; ABZ03313.1; -; Genomic_DNA.
DR EMBL; EU405653; ABZ03314.1; -; Genomic_DNA.
DR EMBL; EU405654; ABZ03315.1; -; Genomic_DNA.
DR EMBL; EU405655; ABZ03316.1; -; Genomic_DNA.
DR EMBL; EU405656; ABZ03317.1; -; Genomic_DNA.
DR EMBL; EU405657; ABZ03318.1; -; Genomic_DNA.
DR EMBL; EU405659; ABZ03320.1; -; Genomic_DNA.
DR EMBL; EU405662; ABZ03323.1; -; Genomic_DNA.
DR EMBL; EU405663; ABZ03324.1; -; Genomic_DNA.
DR EMBL; EU405664; ABZ03325.1; -; Genomic_DNA.
DR EMBL; EU405665; ABZ03326.1; -; Genomic_DNA.
DR EMBL; EU405670; ABZ03331.1; -; Genomic_DNA.
DR EMBL; EU405671; ABZ03332.1; -; Genomic_DNA.
DR EMBL; EU405672; ABZ03333.1; -; Genomic_DNA.
DR EMBL; EU405676; ABZ03337.1; -; Genomic_DNA.
DR EMBL; EU405677; ABZ03338.1; -; Genomic_DNA.
DR EMBL; EU405678; ABZ03339.1; -; Genomic_DNA.
DR EMBL; EU405679; ABZ03340.1; -; Genomic_DNA.
DR EMBL; EU405680; ABZ03341.1; -; Genomic_DNA.
DR EMBL; EU405681; ABZ03342.1; -; Genomic_DNA.
DR EMBL; EU405682; ABZ03343.1; -; Genomic_DNA.
DR EMBL; EU405683; ABZ03344.1; -; Genomic_DNA.
DR EMBL; EU405686; ABZ03347.1; -; Genomic_DNA.
DR EMBL; EU405687; ABZ03348.1; -; Genomic_DNA.
DR EMBL; EU405688; ABZ03349.1; -; Genomic_DNA.
DR EMBL; EU405689; ABZ03350.1; -; Genomic_DNA.
DR EMBL; EU405690; ABZ03351.1; -; Genomic_DNA.
DR EMBL; EU405691; ABZ03352.1; -; Genomic_DNA.
DR EMBL; EU405692; ABZ03353.1; -; Genomic_DNA.
DR EMBL; EU405693; ABZ03354.1; -; Genomic_DNA.
DR EMBL; EU405694; ABZ03355.1; -; Genomic_DNA.
DR EMBL; EU405695; ABZ03356.1; -; Genomic_DNA.
DR EMBL; EU405696; ABZ03357.1; -; Genomic_DNA.
DR EMBL; EU405697; ABZ03358.1; -; Genomic_DNA.
DR EMBL; EU405698; ABZ03359.1; -; Genomic_DNA.
DR EMBL; EU405700; ABZ03361.1; -; Genomic_DNA.
DR EMBL; EU405702; ABZ03363.1; -; Genomic_DNA.
DR EMBL; EU405703; ABZ03364.1; -; Genomic_DNA.
DR EMBL; EU405704; ABZ03365.1; -; Genomic_DNA.
DR EMBL; EU405705; ABZ03366.1; -; Genomic_DNA.
DR EMBL; EU405706; ABZ03367.1; -; Genomic_DNA.
DR EMBL; EU405707; ABZ03368.1; -; Genomic_DNA.
DR EMBL; EU405708; ABZ03369.1; -; Genomic_DNA.
DR EMBL; EU405709; ABZ03370.1; -; Genomic_DNA.
DR EMBL; EU405710; ABZ03371.1; -; Genomic_DNA.
DR EMBL; EU405711; ABZ03372.1; -; Genomic_DNA.
DR EMBL; EU405715; ABZ03376.1; -; Genomic_DNA.
DR EMBL; EU405717; ABZ03378.1; -; Genomic_DNA.
DR RefSeq; NP_196250.1; NM_120715.3.
DR AlphaFoldDB; Q9FNH6; -.
DR IntAct; Q9FNH6; 362.
DR STRING; 3702.AT5G06320.1; -.
DR SwissPalm; Q9FNH6; -.
DR PaxDb; Q9FNH6; -.
DR PRIDE; Q9FNH6; -.
DR ProteomicsDB; 250591; -.
DR EnsemblPlants; AT5G06320.1; AT5G06320.1; AT5G06320.
DR GeneID; 830520; -.
DR Gramene; AT5G06320.1; AT5G06320.1; AT5G06320.
DR KEGG; ath:AT5G06320; -.
DR Araport; AT5G06320; -.
DR TAIR; locus:2164305; AT5G06320.
DR eggNOG; ENOG502QUR9; Eukaryota.
DR HOGENOM; CLU_051752_2_0_1; -.
DR InParanoid; Q9FNH6; -.
DR OMA; IKSWRFK; -.
DR OrthoDB; 1373855at2759; -.
DR PhylomeDB; Q9FNH6; -.
DR PRO; PR:Q9FNH6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FNH6; baseline and differential.
DR GO; GO:0046658; C:anchored component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0071456; P:cellular response to hypoxia; HEP:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0051607; P:defense response to virus; IEP:TAIR.
DR GO; GO:0009617; P:response to bacterium; IEP:UniProtKB.
DR GO; GO:0009751; P:response to salicylic acid; IEP:UniProtKB.
DR GO; GO:0009611; P:response to wounding; IEP:UniProtKB.
DR InterPro; IPR004864; LEA_2.
DR InterPro; IPR044839; NDR1-like.
DR PANTHER; PTHR31415; PTHR31415; 1.
DR Pfam; PF03168; LEA_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Plant defense; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..231
FT /note="NDR1/HIN1-like protein 3"
FT /id="PRO_0000435650"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 135
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 145
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 215
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CONFLICT 117
FT /note="Missing (in Ref. 1; AAF88023 and 2; AAN60261)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 231 AA; 25947 MW; ACBB6BF3581FEF3B CRC64;
MADLNGAYYG PSIPPPKKVS HSHGRRGGGC GCLGDCLGCC GCCILSVIFN ILITIAVLLG
IAALIIWLIF RPNAIKFHVT DAKLTEFTLD PTNNLRYNLD LNFTIRNPNR RIGVYYDEIE
VRGYYGDQRF GMSNNISKFY QGHKNTTVVG TKLVGQQLVL LDGGERKDLN EDVNSQIYRI
DAKLRLKIRF KFGLIKSWRF KPKIKCDLKV PLTSNSTSGF VFQPTKCDVD F
