NHLC3_HUMAN
ID NHLC3_HUMAN Reviewed; 347 AA.
AC Q5JS37; B2RTZ2; B4DTL0; Q69YI9;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=NHL repeat-containing protein 3;
DE Flags: Precursor;
GN Name=NHLRC3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15057823; DOI=10.1038/nature02379;
RA Dunham A., Matthews L.H., Burton J., Ashurst J.L., Howe K.L.,
RA Ashcroft K.J., Beare D.M., Burford D.C., Hunt S.E., Griffiths-Jones S.,
RA Jones M.C., Keenan S.J., Oliver K., Scott C.E., Ainscough R., Almeida J.P.,
RA Ambrose K.D., Andrews D.T., Ashwell R.I.S., Babbage A.K., Bagguley C.L.,
RA Bailey J., Bannerjee R., Barlow K.F., Bates K., Beasley H., Bird C.P.,
RA Bray-Allen S., Brown A.J., Brown J.Y., Burrill W., Carder C., Carter N.P.,
RA Chapman J.C., Clamp M.E., Clark S.Y., Clarke G., Clee C.M., Clegg S.C.,
RA Cobley V., Collins J.E., Corby N., Coville G.J., Deloukas P., Dhami P.,
RA Dunham I., Dunn M., Earthrowl M.E., Ellington A.G., Faulkner L.,
RA Frankish A.G., Frankland J., French L., Garner P., Garnett J.,
RA Gilbert J.G.R., Gilson C.J., Ghori J., Grafham D.V., Gribble S.M.,
RA Griffiths C., Hall R.E., Hammond S., Harley J.L., Hart E.A., Heath P.D.,
RA Howden P.J., Huckle E.J., Hunt P.J., Hunt A.R., Johnson C., Johnson D.,
RA Kay M., Kimberley A.M., King A., Laird G.K., Langford C.J., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Lloyd C., Loveland J.E., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., McLaren S.J., McMurray A., Milne S.,
RA Moore M.J.F., Nickerson T., Palmer S.A., Pearce A.V., Peck A.I., Pelan S.,
RA Phillimore B., Porter K.M., Rice C.M., Searle S., Sehra H.K., Shownkeen R.,
RA Skuce C.D., Smith M., Steward C.A., Sycamore N., Tester J., Thomas D.W.,
RA Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P.,
RA Whitehead S.L., Willey D.L., Wilming L., Wray P.W., Wright M.W., Young L.,
RA Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Beck S., Bentley D.R.,
RA Rogers J., Ross M.T.;
RT "The DNA sequence and analysis of human chromosome 13.";
RL Nature 428:522-528(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-129 (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5JS37-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5JS37-2; Sequence=VSP_042858;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK300260; BAG62022.1; -; mRNA.
DR EMBL; AL445590; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471075; EAX08613.1; -; Genomic_DNA.
DR EMBL; CH471075; EAX08615.1; -; Genomic_DNA.
DR EMBL; BC140877; AAI40878.1; -; mRNA.
DR EMBL; BC140878; AAI40879.1; -; mRNA.
DR EMBL; AL833329; CAH10395.1; -; mRNA.
DR CCDS; CCDS31961.1; -. [Q5JS37-1]
DR CCDS; CCDS31962.1; -. [Q5JS37-2]
DR RefSeq; NP_001012772.1; NM_001012754.3. [Q5JS37-1]
DR RefSeq; NP_001017370.1; NM_001017370.2. [Q5JS37-2]
DR AlphaFoldDB; Q5JS37; -.
DR SMR; Q5JS37; -.
DR BioGRID; 132506; 54.
DR IntAct; Q5JS37; 3.
DR STRING; 9606.ENSP00000368920; -.
DR GlyGen; Q5JS37; 5 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q5JS37; -.
DR PhosphoSitePlus; Q5JS37; -.
DR BioMuta; NHLRC3; -.
DR DMDM; 74741946; -.
DR EPD; Q5JS37; -.
DR jPOST; Q5JS37; -.
DR MassIVE; Q5JS37; -.
DR MaxQB; Q5JS37; -.
DR PaxDb; Q5JS37; -.
DR PeptideAtlas; Q5JS37; -.
DR PRIDE; Q5JS37; -.
DR ProteomicsDB; 63133; -. [Q5JS37-1]
DR ProteomicsDB; 63134; -. [Q5JS37-2]
DR Antibodypedia; 2743; 99 antibodies from 17 providers.
DR DNASU; 387921; -.
DR Ensembl; ENST00000379599.6; ENSP00000368919.2; ENSG00000188811.14. [Q5JS37-2]
DR Ensembl; ENST00000379600.8; ENSP00000368920.3; ENSG00000188811.14. [Q5JS37-1]
DR GeneID; 387921; -.
DR KEGG; hsa:387921; -.
DR MANE-Select; ENST00000379600.8; ENSP00000368920.3; NM_001012754.4; NP_001012772.1.
DR UCSC; uc001uxc.5; human. [Q5JS37-1]
DR CTD; 387921; -.
DR GeneCards; NHLRC3; -.
DR HGNC; HGNC:33751; NHLRC3.
DR HPA; ENSG00000188811; Low tissue specificity.
DR neXtProt; NX_Q5JS37; -.
DR OpenTargets; ENSG00000188811; -.
DR PharmGKB; PA162397544; -.
DR VEuPathDB; HostDB:ENSG00000188811; -.
DR eggNOG; KOG2177; Eukaryota.
DR GeneTree; ENSGT00390000008657; -.
DR HOGENOM; CLU_037899_2_0_1; -.
DR InParanoid; Q5JS37; -.
DR OMA; SEDGPYS; -.
DR PhylomeDB; Q5JS37; -.
DR TreeFam; TF331018; -.
DR PathwayCommons; Q5JS37; -.
DR Reactome; R-HSA-6798695; Neutrophil degranulation.
DR SignaLink; Q5JS37; -.
DR BioGRID-ORCS; 387921; 24 hits in 1082 CRISPR screens.
DR ChiTaRS; NHLRC3; human.
DR GenomeRNAi; 387921; -.
DR Pharos; Q5JS37; Tdark.
DR PRO; PR:Q5JS37; -.
DR Proteomes; UP000005640; Chromosome 13.
DR RNAct; Q5JS37; protein.
DR Bgee; ENSG00000188811; Expressed in oocyte and 185 other tissues.
DR ExpressionAtlas; Q5JS37; baseline and differential.
DR Genevisible; Q5JS37; HS.
DR GO; GO:0035578; C:azurophil granule lumen; TAS:Reactome.
DR GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR Gene3D; 2.120.10.30; -; 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR001258; NHL_repeat.
DR Pfam; PF01436; NHL; 1.
DR PROSITE; PS51125; NHL; 4.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Reference proteome; Repeat; Secreted;
KW Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..347
FT /note="NHL repeat-containing protein 3"
FT /id="PRO_0000317194"
FT REPEAT 47..93
FT /note="NHL 1"
FT REPEAT 150..196
FT /note="NHL 2"
FT REPEAT 200..243
FT /note="NHL 3"
FT REPEAT 294..338
FT /note="NHL 4"
FT CARBOHYD 32
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 101
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 206
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 278
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 129..195
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_042858"
FT VARIANT 221
FT /note="L -> R (in dbSNP:rs9603498)"
FT /id="VAR_051233"
SQ SEQUENCE 347 AA; 38283 MW; 7CF110AF0FC10489 CRC64;
MARFWVCVAG AGFFLAFLVL HSRFCGSPVL RNFTFAVSWR TEKILYRLDV GWPKHPEYFT
GTTFCVAVDS LNGLVYIGQR GDNIPKILVF TEDGYFLRAW NYTVDTPHGI FAASTLYEQS
VWITDVGSGF FGHTVKKYSS FGDLVQVLGT PGKKGTSLNP LQFDNPAELY VEDTGDIYIV
DGDGGLNNRL IKLSQDFMIL WLHGENGTGP AKFNIPHSVT LDSAGRVWVA DRGNKRIQVF
DKDTGEWLGA WNNCFTEEGP SSVRFTPDGK YLIVAQLNLS RLSVVAAPPV GSIGECSVIS
TIQLADQVLP HLLEVDRKTG AVYVAEIGAK QVQKYVPLNS YVPSFGS
