NHP2_ARATH
ID NHP2_ARATH Reviewed; 156 AA.
AC Q9LEY9;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=H/ACA ribonucleoprotein complex subunit 2-like protein;
DE AltName: Full=Nhp2-like protein;
GN OrderedLocusNames=At5g08180; ORFNames=T22D6.120;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Required for ribosome biogenesis. Part of a complex which
CC catalyzes pseudouridylation of rRNA. This involves the isomerization of
CC uridine such that the ribose is subsequently attached to C5, instead of
CC the normal N1. Pseudouridine ('psi') residues may serve to stabilize
CC the conformation of rRNAs (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the small nucleolar ribonucleoprotein particle
CC containing H/ACA-type snoRNAs (H/ACA snoRNPs). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the eukaryotic ribosomal protein eL8 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL357612; CAB93719.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91262.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91263.1; -; Genomic_DNA.
DR EMBL; AY050477; AAK91490.1; -; mRNA.
DR EMBL; AF378885; AAK55688.1; -; mRNA.
DR PIR; T50503; T50503.
DR RefSeq; NP_001190260.1; NM_001203331.1.
DR RefSeq; NP_196435.1; NM_120901.4.
DR AlphaFoldDB; Q9LEY9; -.
DR SMR; Q9LEY9; -.
DR BioGRID; 15992; 5.
DR IntAct; Q9LEY9; 1.
DR STRING; 3702.AT5G08180.2; -.
DR iPTMnet; Q9LEY9; -.
DR PaxDb; Q9LEY9; -.
DR PRIDE; Q9LEY9; -.
DR ProteomicsDB; 249386; -.
DR EnsemblPlants; AT5G08180.1; AT5G08180.1; AT5G08180.
DR EnsemblPlants; AT5G08180.2; AT5G08180.2; AT5G08180.
DR GeneID; 830714; -.
DR Gramene; AT5G08180.1; AT5G08180.1; AT5G08180.
DR Gramene; AT5G08180.2; AT5G08180.2; AT5G08180.
DR KEGG; ath:AT5G08180; -.
DR Araport; AT5G08180; -.
DR TAIR; locus:2181514; AT5G08180.
DR eggNOG; KOG3167; Eukaryota.
DR HOGENOM; CLU_084513_1_1_1; -.
DR InParanoid; Q9LEY9; -.
DR OMA; IDVYSHI; -.
DR OrthoDB; 1538526at2759; -.
DR PhylomeDB; Q9LEY9; -.
DR PRO; PR:Q9LEY9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LEY9; baseline and differential.
DR Genevisible; Q9LEY9; AT.
DR GO; GO:0031429; C:box H/ACA snoRNP complex; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0005732; C:sno(s)RNA-containing ribonucleoprotein complex; ISS:UniProtKB.
DR GO; GO:0034513; F:box H/ACA snoRNA binding; IBA:GO_Central.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000469; P:cleavage involved in rRNA processing; IBA:GO_Central.
DR GO; GO:0000470; P:maturation of LSU-rRNA; IBA:GO_Central.
DR GO; GO:0031118; P:rRNA pseudouridine synthesis; ISS:UniProtKB.
DR GO; GO:0031120; P:snRNA pseudouridine synthesis; IBA:GO_Central.
DR Gene3D; 3.30.1330.30; -; 1.
DR InterPro; IPR002415; H/ACA_rnp_Nhp2-like.
DR InterPro; IPR029064; L30e-like.
DR InterPro; IPR004038; Ribosomal_L7Ae/L30e/S12e/Gad45.
DR InterPro; IPR018492; Ribosomal_L7Ae/L8/Nhp2.
DR Pfam; PF01248; Ribosomal_L7Ae; 1.
DR PRINTS; PR00881; L7ARS6FAMILY.
DR PRINTS; PR00883; NUCLEARHMG.
DR SUPFAM; SSF55315; SSF55315; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ribonucleoprotein; Ribosome biogenesis;
KW RNA-binding; rRNA processing.
FT CHAIN 1..156
FT /note="H/ACA ribonucleoprotein complex subunit 2-like
FT protein"
FT /id="PRO_0000136774"
SQ SEQUENCE 156 AA; 16948 MW; CC8B7C196E92442A CRC64;
MGSDTEAEKS IQKEKKKYAI TLAPIAKPLA GKKLQKRTFK LIQKAAGKKC LKRGVKEVVK
SIRRGQKGLC VIAGNISPID VITHLPILCE EAGVPYVYVP SKEDLAQAGA TKRPTCCVLV
MLKPAKGDLT AEELAKLKTD YEQVSDDIKE LATSVI
