ID   NHP6_SCHPO              Reviewed;         108 AA.
AC   P87057;
DT   11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Non-histone chromosomal protein 6;
GN   Name=nhp6; ORFNames=SPAC57A10.09c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=26582768; DOI=10.15252/embr.201540476;
RA   Gal C., Murton H.E., Subramanian L., Whale A.J., Moore K.M.,
RA   Paszkiewicz K., Codlin S., Baehler J., Creamer K.M., Partridge J.F.,
RA   Allshire R.C., Kent N.A., Whitehall S.K.;
RT   "Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome
RT   occupancy and organisation.";
RL   EMBO Rep. 17:79-93(2016).
CC   -!- FUNCTION: DNA-binding protein that induces severe bending of DNA.
CC       Required for DNA-binding by the FACT complex, a general chromatin
CC       factor that acts to reorganize nucleosomes. The FACT complex is
CC       involved in multiple processes that require DNA as a template such as
CC       mRNA elongation, DNA replication and DNA repair. Also augments the
CC       fidelity of transcription by RNA polymerase III independently of any
CC       role in the FACT complex (By similarity).
CC       {ECO:0000250|UniProtKB:P11632}.
CC   -!- SUBUNIT: Weakly associates with the stable spt16-pob3 heterodimer to
CC       form the FACT complex. {ECO:0000250|UniProtKB:P11632}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267}.
CC       Chromosome {ECO:0000250|UniProtKB:P11632}.
CC   -!- DISRUPTION PHENOTYPE: Double-knockout with abo1 exacerbates the
CC       sensitivity of the abo1-knockout to methyl methanesulfonate (DNA
CC       damaging agent). {ECO:0000269|PubMed:26582768}.
CC   -!- SIMILARITY: Belongs to the NHP6 family. {ECO:0000305}.
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DR   EMBL; CU329670; CAB08172.1; -; Genomic_DNA.
DR   PIR; T38936; T38936.
DR   RefSeq; NP_593314.1; NM_001018745.2.
DR   AlphaFoldDB; P87057; -.
DR   SMR; P87057; -.
DR   BioGRID; 279323; 27.
DR   STRING; 4896.SPAC57A10.09c.1; -.
DR   MaxQB; P87057; -.
DR   PaxDb; P87057; -.
DR   PRIDE; P87057; -.
DR   EnsemblFungi; SPAC57A10.09c.1; SPAC57A10.09c.1:pep; SPAC57A10.09c.
DR   GeneID; 2542878; -.
DR   KEGG; spo:SPAC57A10.09c; -.
DR   PomBase; SPAC57A10.09c; nhp6.
DR   VEuPathDB; FungiDB:SPAC57A10.09c; -.
DR   eggNOG; KOG0381; Eukaryota.
DR   HOGENOM; CLU_082854_10_0_1; -.
DR   InParanoid; P87057; -.
DR   OMA; QKAPYEA; -.
DR   PhylomeDB; P87057; -.
DR   Reactome; R-SPO-140342; Apoptosis induced DNA fragmentation.
DR   Reactome; R-SPO-163282; Mitochondrial transcription initiation.
DR   Reactome; R-SPO-5620971; Pyroptosis.
DR   Reactome; R-SPO-5686938; Regulation of TLR by endogenous ligand.
DR   Reactome; R-SPO-6798695; Neutrophil degranulation.
DR   PRO; PR:P87057; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0000785; C:chromatin; ISO:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0008301; F:DNA binding, bending; IBA:GO_Central.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006334; P:nucleosome assembly; ISO:PomBase.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:PomBase.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   Pfam; PF00505; HMG_box; 1.
DR   SMART; SM00398; HMG; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
DR   PROSITE; PS50118; HMG_BOX_2; 1.
PE   3: Inferred from homology;
KW   Chromosome; DNA damage; DNA repair; DNA-binding; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..108
FT                   /note="Non-histone chromosomal protein 6"
FT                   /id="PRO_0000245222"
FT   DNA_BIND        16..84
FT                   /note="HMG box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          61..108
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        61..89
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   108 AA;  12361 MW;  A92F34A98995D6B6 CRC64;
     MPRAAKSSRK KDPNTPKRNM SAFMFFSIEN REKMKTDNPD ATFGQLGSLL GKRWKELTST
     EREPYEEKAR QDKERYERER KEYDTKLANG EKTGKASAPA AAAAAKEE