NHR14_CAEEL
ID NHR14_CAEEL Reviewed; 435 AA.
AC O02151; Q8MPT6; Q9UAN4;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 3.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Nuclear hormone receptor family member nhr-14;
GN Name=nhr-14; ORFNames=T01B10.4;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 148-435 (ISOFORM A), AND FUNCTION.
RC STRAIN=Bristol N2;
RX PubMed=10022975;
RA Sluder A.E., Mathews S.W., Hough D., Yin V.P., Maina C.V.;
RT "The nuclear receptor superfamily has undergone extensive proliferation and
RT diversification in nematodes.";
RL Genome Res. 9:103-120(1999).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP GLY-33 AND 49-TRP--SER-435.
RX PubMed=31532389; DOI=10.7554/elife.44674;
RA Rajan M., Anderson C.P., Rindler P.M., Romney S.J.,
RA Ferreira Dos Santos M.C., Gertz J., Leibold E.A.;
RT "NHR-14 loss of function couples intestinal iron uptake with innate
RT immunity in C. elegans through PQM-1 signaling.";
RL Elife 8:0-0(2019).
CC -!- FUNCTION: Orphan nuclear receptor (PubMed:10022975). Transcriptional
CC repressor of intestinal metal transporter smf-3 and genes of the innate
CC immune response (PubMed:31532389). Inhibits nuclear localization of
CC transcription factor pqm-1; in response to pathogen stress, may
CC facilitate translocation of pqm-1, leading to transcriptional
CC activation of genes involved in innate immunity and iron uptake
CC (PubMed:31532389). {ECO:0000269|PubMed:10022975,
CC ECO:0000269|PubMed:31532389}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=O02151-1; Sequence=Displayed;
CC Name=b;
CC IsoId=O02151-2; Sequence=VSP_007344;
CC -!- TISSUE SPECIFICITY: Expressed in intestine and head neurons in young
CC adults. {ECO:0000269|PubMed:31532389}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes enhanced
CC resistance to infection with the Gram-negative bacterium P.aeruginosa
CC (PubMed:31532389). Knockdown suppresses the developmental delay
CC phenotype of hif-1 mutants when grown under iron limiting conditions
CC (PubMed:31532389). {ECO:0000269|PubMed:31532389}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FO081164; CCD69597.1; -; Genomic_DNA.
DR EMBL; FO081164; CCD69598.1; -; Genomic_DNA.
DR EMBL; AF083229; AAD03687.1; -; mRNA.
DR PIR; T43353; T43353.
DR RefSeq; NP_741860.2; NM_171742.3. [O02151-1]
DR RefSeq; NP_741861.2; NM_171743.3. [O02151-2]
DR AlphaFoldDB; O02151; -.
DR SMR; O02151; -.
DR BioGRID; 46023; 2.
DR STRING; 6239.T01B10.4a.2; -.
DR EPD; O02151; -.
DR PaxDb; O02151; -.
DR PRIDE; O02151; -.
DR EnsemblMetazoa; T01B10.4a.1; T01B10.4a.1; WBGene00003613. [O02151-1]
DR EnsemblMetazoa; T01B10.4b.1; T01B10.4b.1; WBGene00003613. [O02151-2]
DR GeneID; 181102; -.
DR KEGG; cel:CELE_T01B10.4; -.
DR UCSC; T01B10.4b; c. elegans. [O02151-1]
DR CTD; 181102; -.
DR WormBase; T01B10.4a; CE33818; WBGene00003613; nhr-14. [O02151-1]
DR WormBase; T01B10.4b; CE33819; WBGene00003613; nhr-14. [O02151-2]
DR eggNOG; KOG4215; Eukaryota.
DR InParanoid; O02151; -.
DR OMA; FAYMSNT; -.
DR OrthoDB; 622732at2759; -.
DR PhylomeDB; O02151; -.
DR PRO; PR:O02151; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00003613; Expressed in larva and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990239; F:steroid hormone binding; IDA:WormBase.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:UniProtKB.
DR GO; GO:0055072; P:iron ion homeostasis; IMP:UniProtKB.
DR GO; GO:1900181; P:negative regulation of protein localization to nucleus; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus; Receptor;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..435
FT /note="Nuclear hormone receptor family member nhr-14"
FT /id="PRO_0000053767"
FT DOMAIN 131..355
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 17..92
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 20..40
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 56..80
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 91..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..110
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 111..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 420..435
FT /note="EFGCHLQSMPVKVILS -> YNRNSDVIFSQCPSK (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_007344"
FT MUTAGEN 33
FT /note="G->R: In qa6910; strong suppressor of the
FT developmental delay phenotype of hif-1 mutants when grown
FT under iron limiting conditions."
FT /evidence="ECO:0000269|PubMed:31532389"
FT MUTAGEN 49..435
FT /note="Missing: In qa6909; strong suppressor of the
FT developmental delay phenotype of hif-1 mutants when grown
FT under iron limiting conditions."
FT /evidence="ECO:0000269|PubMed:31532389"
SQ SEQUENCE 435 AA; 48560 MW; F0F6E770ED804536 CRC64;
MDFLISTSLS ESTSTSADFC VVCGDKAIGK HYGAVACNGC KGFFRRSVWQ NLQYTCRFNK
QCNIDKDHRN ACRYCRFQKC LADGMKPEAI QNERDRIGST KRRKRSGANS ENNSDSEGTP
SPKIEVMGNS VSRKLIEMLL DIEHRLASNQ SMNALLRDES EMKNSRQRAV NYLIGWTNML
HPLPEVPLAD KVLLLKKFSS AFTLLGTLQR SMALPHFVLP NDQVLSISAS HPPELFEALT
RIIDELLTPL RRLRTDHAEF SCLKALLLLN PDVVGISNNT RERIREARDA LLKTLFAYMS
NTQNSIDASL RVSSLLMIIP SLISVSSSIM EFPALSDLFG LGDVIKRDTI SPKIETPPLE
MKPMMPKIAQ PPVTSAPTVP TNIMMNKDLI SQIMNNPQLF PLLPMPQTAS PPMSFMGQSE
FGCHLQSMPV KVILS
