NHR60_CAEEL
ID NHR60_CAEEL Reviewed; 443 AA.
AC O17898; Q9GTF1;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Nuclear hormone receptor family member nhr-60 {ECO:0000312|WormBase:F57A10.5};
GN Name=nhr-60 {ECO:0000312|WormBase:F57A10.5};
GN ORFNames=F57A10.5 {ECO:0000312|WormBase:F57A10.5};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=17579999;
RA Simeckova K., Brozova E., Vohanka J., Pohludka M., Kostrouch Z.,
RA Krause M.W., Rall J.E., Kostrouchova M.;
RT "Supplementary nuclear receptor NHR-60 is required for normal embryonic and
RT early larval development of Caenorhabditis elegans.";
RL Folia Biol. (Praha) 53:85-96(2007).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000312|EMBL:AAG15157.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 2-443.
RX PubMed=15983867; DOI=10.1007/s00239-004-0175-8;
RA Robinson-Rechavi M., Maina C.V., Gissendanner C.R., Laudet V., Sluder A.;
RT "Explosive lineage-specific expansion of the orphan nuclear receptor HNF4
RT in nematodes.";
RL J. Mol. Evol. 60:577-586(2005).
CC -!- FUNCTION: Orphan nuclear receptor (Potential). Required for embryonic
CC and larval morphogenesis and probably for seam cell positioning and
CC migration. {ECO:0000269|PubMed:17579999, ECO:0000305|PubMed:17579999}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17579999}.
CC Note=Localized in the nucleus from the one-cell stage of development
CC onwards. Localizes to the nuclear periphery of all cells.
CC {ECO:0000269|PubMed:17579999}.
CC -!- DEVELOPMENTAL STAGE: Expressed from embryogenesis onwards with high
CC expression during the larval stages of development, peaking at the L3
CC stage and decreasing at the L4 stage of larval development.
CC Ubiquitously expressed, but expression in seam cells begins in
CC embryonic precursors soon after fertilization and is most prominent in
CC larval stages of development. Expression is also prominent in the germ
CC line during the L1 stage of larval development. Also expressed in
CC pharyngeal gland cells, VC4 and VC5 neurons, and in the hermaphrodite
CC uterine vulval uv1 cells. {ECO:0000269|PubMed:17579999}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in arrest at the
CC two-fold stage of embryogenesis in the majority of embryos. These
CC embryos have incomplete ventral closure and morphological defects
CC including elongation defects, and either absent or irregularly placed
CC seam cells in the head and tail regions. Surviving larvae also exhibit
CC morphological defects and contain vacuoles.
CC {ECO:0000269|PubMed:17579999}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family.
CC {ECO:0000255|RuleBase:RU004334}.
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DR EMBL; AF273808; AAG15157.1; -; mRNA.
DR EMBL; BX284605; CAB09422.1; -; Genomic_DNA.
DR PIR; T22817; T22817.
DR RefSeq; NP_506930.1; NM_074529.1.
DR AlphaFoldDB; O17898; -.
DR DIP; DIP-27121N; -.
DR STRING; 6239.F57A10.5; -.
DR PaxDb; O17898; -.
DR EnsemblMetazoa; F57A10.5.1; F57A10.5.1; WBGene00003650.
DR GeneID; 180059; -.
DR KEGG; cel:CELE_F57A10.5; -.
DR UCSC; F57A10.5; c. elegans.
DR CTD; 180059; -.
DR WormBase; F57A10.5; CE16151; WBGene00003650; nhr-60.
DR eggNOG; ENOG502TFFE; Eukaryota.
DR GeneTree; ENSGT00970000195878; -.
DR HOGENOM; CLU_007368_3_1_1; -.
DR InParanoid; O17898; -.
DR OMA; ALQNIEC; -.
DR OrthoDB; 1683825at2759; -.
DR PhylomeDB; O17898; -.
DR PRO; PR:O17898; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00003650; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005635; C:nuclear envelope; IDA:WormBase.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Receptor; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..443
FT /note="Nuclear hormone receptor family member nhr-60"
FT /evidence="ECO:0000305"
FT /id="PRO_0000437683"
FT DOMAIN 196..439
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 44..121
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 47..67
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 83..104
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 322
FT /note="N -> S (in Ref. 3; AAG15157)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 443 AA; 50309 MW; AA65D149CCA931A6 CRC64;
MIQSSSSISQ DSLDLPSILS TFSADEPEDE PSPTAVKSTK PQRPTECLIC GNSANGHHYD
VASCNGCKTF FRRMCVSDRS FECKAKGDCF DLTKRKVPLK CRACRHQKCI SVGMNPLAME
VDEKAASSSN FKKLVKRAQD LEPDDDCQVT AVVNKMQIVK PVDSIESKMQ NITDMLVYLE
TKVERFRKSA YNPHWNEFSG LEYLLESECR IGFGDRFGPM PGWPLRRDQL GPPRLPKPPS
PGKPRDSQHS QNIKQWFFYN LLTTVEYAKT FMFFHRLSSR DKLILTRHVA LACTNLHISY
SSIRRNLEVI IEPDGSEPMT FNDTHYSASV MSVAPLIRCQ IKNIEYLLLK AIVLCNPAVP
DLSAKSQEII SLERVKYADA LFNYCLRSRS DGPPHFAQLI QIIDVLERQQ RMQKDLHLLL
VAPKVAKLPK DLVMRVIEDI MDS
