NHR67_CAEEL
ID NHR67_CAEEL Reviewed; 416 AA.
AC Q9XVV3;
DT 16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Nuclear hormone receptor family member nhr-67;
GN Name=nhr-67; ORFNames=C08F8.8;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION.
RX PubMed=18179707; DOI=10.1186/1471-2199-9-2;
RA DeMeo S.D., Lombel R.M., Cronin M., Smith E.L., Snowflack D.R., Reinert K.,
RA Clever S., Wightman B.;
RT "Specificity of DNA-binding by the FAX-1 and NHR-67 nuclear receptors of
RT Caenorhabditis elegans is partially mediated via a subclass-specific P-box
RT residue.";
RL BMC Mol. Biol. 9:2-2(2008).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=19906858; DOI=10.1242/dev.035477;
RA Kato M., Sternberg P.W.;
RT "The C. elegans tailless/Tlx homolog nhr-67 regulates a stage-specific
RT program of linker cell migration in male gonadogenesis.";
RL Development 136:3907-3915(2009).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22363008; DOI=10.1126/science.1215156;
RA Blum E.S., Abraham M.C., Yoshimura S., Lu Y., Shaham S.;
RT "Control of nonapoptotic developmental cell death in Caenorhabditis elegans
RT by a polyglutamine-repeat protein.";
RL Science 335:970-973(2012).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=27472063; DOI=10.1038/cdd.2016.77;
RA Malin J.A., Kinet M.J., Abraham M.C., Blum E.S., Shaham S.;
RT "Transcriptional control of non-apoptotic developmental cell death in C.
RT elegans.";
RL Cell Death Differ. 23:1985-1994(2016).
CC -!- FUNCTION: Orphan nuclear receptor that binds DNA containing an extended
CC core motif half-site sequence 5'-AAGTCA-3' (PubMed:18179707,
CC PubMed:19906858). In males, plays an essential role in the migration of
CC the linker cell which guides gonad elongation during the L3 and L4
CC stages of larval development by negatively regulating the expression of
CC netrin receptor unc-5 at the mid-L3 stage (PubMed:22363008,
CC PubMed:19906858). Involved in the regulation of non-apoptotic cell
CC death in the linker cell, acting upstream of or in parallel to
CC transcription factor hsf-1 (PubMed:27472063).
CC {ECO:0000269|PubMed:18179707, ECO:0000269|PubMed:19906858,
CC ECO:0000269|PubMed:22363008, ECO:0000269|PubMed:27472063}.
CC -!- INTERACTION:
CC Q9XVV3; O02482: unc-37; NbExp=3; IntAct=EBI-314697, EBI-314716;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407}.
CC -!- TISSUE SPECIFICITY: Expressed in linker cell.
CC {ECO:0000269|PubMed:19906858}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in males prevents
CC migration of the linker cell (LC) during larval development resulting
CC in abnormal gonad migration (PubMed:9851916, PubMed:19906858). At the
CC L3-to-L4 molt stage, the LC fails to reach the P7.p hypodermal cell and
CC fails to perform the turn from the dorsal to the ventral side of the
CC body. During the late L4 stage, the LC turns ventrally but stays
CC farther behind its normal position (PubMed:9851916, PubMed:19906858).
CC In addition, LC polarization, which normally occurs during the L3-L4
CC stages, is severely delayed and mig-2 polarization to the adherent side
CC of thr LC is impaired (PubMed:19906858). In the LC, expression of unc-5
CC at the L3 and L4 larval stages is increased and expression of zmp-1 is
CC absent at L4 stage (PubMed:19906858). Prevents non-apoptotic cell death
CC in the LC (PubMed:27472063). Knockdown enhances LC survival on egl-20,
CC eor-1, or lin-29 mutant backgrounds (PubMed:27472063). Causes
CC precocious expression of let-70 in the LC during larval stage L3
CC (PubMed:27472063). RNAi-mediated knockdown in hermaphrodites causes no
CC defect in distal tip cell migration (PubMed:19906858).
CC {ECO:0000269|PubMed:19906858, ECO:0000269|PubMed:27472063,
CC ECO:0000269|PubMed:9851916}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family.
CC {ECO:0000305}.
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DR EMBL; Z73103; CAA97428.1; -; Genomic_DNA.
DR PIR; T19102; T19102.
DR RefSeq; NP_502094.1; NM_069693.3.
DR AlphaFoldDB; Q9XVV3; -.
DR SMR; Q9XVV3; -.
DR BioGRID; 43123; 34.
DR DIP; DIP-25535N; -.
DR IntAct; Q9XVV3; 33.
DR STRING; 6239.C08F8.8; -.
DR EPD; Q9XVV3; -.
DR PaxDb; Q9XVV3; -.
DR EnsemblMetazoa; C08F8.8.1; C08F8.8.1; WBGene00003657.
DR GeneID; 178024; -.
DR KEGG; cel:CELE_C08F8.8; -.
DR UCSC; C08F8.8; c. elegans.
DR CTD; 178024; -.
DR WormBase; C08F8.8; CE18491; WBGene00003657; nhr-67.
DR eggNOG; KOG3575; Eukaryota.
DR HOGENOM; CLU_055512_0_0_1; -.
DR InParanoid; Q9XVV3; -.
DR OMA; RYISCMV; -.
DR OrthoDB; 870262at2759; -.
DR PhylomeDB; Q9XVV3; -.
DR SignaLink; Q9XVV3; -.
DR PRO; PR:Q9XVV3; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00003657; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:WormBase.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IMP:WormBase.
DR GO; GO:0016477; P:cell migration; IMP:UniProtKB.
DR GO; GO:0035262; P:gonad morphogenesis; IMP:WormBase.
DR GO; GO:2000134; P:negative regulation of G1/S transition of mitotic cell cycle; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:WormBase.
DR GO; GO:0012501; P:programmed cell death; IMP:UniProtKB.
DR GO; GO:0030334; P:regulation of cell migration; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Metal-binding; Nucleus; Receptor; Reference proteome;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..416
FT /note="Nuclear hormone receptor family member nhr-67"
FT /id="PRO_0000053794"
FT DNA_BIND 18..98
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 21..41
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 57..86
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 331..398
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 335..349
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 372..398
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 416 AA; 46771 MW; 4BC549E9C26BEA28 CRC64;
MMTAVSQMSV PSSRILLDVD CRVCEDHSSG KHYSIFSCDG CAGFFKRSIR RHRQYVCKNK
GSPSEGQCKV DKTHRNQCRA CRLRKCLEIG MNKDAVQHER GPRNSSLRRQ QMMFDHGSSP
NSPEMGSESD AIILPTSSMN RDTVAGTAAR IFFALVGFCQ NPLNGVPKER QMTMFQQNWA
ALLVLHATET RAITSKQIRT ETISGSSEQR NAVANAFEII ERLQLDNREY MMLKHFTMWR
DTPSAIQIVF QLASIQNFTH RTEPTRYIQC INAIAAIPTT SIIDVLFRPS IGSASMPRLI
QDMFKPPQQP TPTSLFPMAN FNLNFLLKQE KTETEEGEDI EEEDDATSSN QFDENSSTDD
RSVGELDPVQ LFLALNSSTQ PSSASSPSSS RPRHSIRSIT ELLSIQEEES VNVEEV
