NHSL1_MOUSE
ID NHSL1_MOUSE Reviewed; 1587 AA.
AC Q8CAF4; Q5DTY4; Q6P6Y7; Q8C8Z7; Q8K0R2; Q91YY6; Q9CYM2;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 3.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=NHS-like protein 1;
GN Name=Nhsl1; Synonyms=Kiaa1357;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-526 (ISOFORM 3), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 1259-1587 (ISOFORM 1).
RC STRAIN=Czech II, and FVB/N-3; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-446 AND 1140-1587 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, Hypothalamus, and Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1107-1587 (ISOFORM 1).
RC TISSUE=Kidney;
RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The
RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by
RT screening of terminal sequences of cDNA clones randomly sampled from size-
RT fractionated libraries.";
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1373; SER-1375 AND THR-1379,
RP AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-24; SER-328; SER-563;
RP SER-629; SER-1079; SER-1373; SER-1375 AND THR-1379, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CAF4-1; Sequence=Displayed;
CC Name=3;
CC IsoId=Q8CAF4-3; Sequence=VSP_034261, VSP_034263;
CC -!- SIMILARITY: Belongs to the NHS family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH13565.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAH30842.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the C-terminal part.; Evidence={ECO:0000305};
CC Sequence=AAH61949.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB30793.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
CC Sequence=BAC31551.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE22306.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC153560; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC153561; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC013565; AAH13565.2; ALT_INIT; mRNA.
DR EMBL; BC030842; AAH30842.1; ALT_SEQ; mRNA.
DR EMBL; BC061949; AAH61949.1; ALT_INIT; mRNA.
DR EMBL; AK038892; BAC30160.2; -; mRNA.
DR EMBL; AK017530; BAB30793.1; ALT_SEQ; mRNA.
DR EMBL; AK043447; BAC31551.1; ALT_INIT; mRNA.
DR EMBL; AK134837; BAE22306.1; ALT_INIT; mRNA.
DR EMBL; AK220386; BAD90251.1; -; mRNA.
DR CCDS; CCDS35853.1; -. [Q8CAF4-1]
DR CCDS; CCDS48508.1; -. [Q8CAF4-3]
DR RefSeq; NP_001157064.1; NM_001163592.1. [Q8CAF4-3]
DR RefSeq; NP_775566.3; NM_173390.3. [Q8CAF4-1]
DR AlphaFoldDB; Q8CAF4; -.
DR BioGRID; 229663; 25.
DR IntAct; Q8CAF4; 24.
DR STRING; 10090.ENSMUSP00000040799; -.
DR iPTMnet; Q8CAF4; -.
DR PhosphoSitePlus; Q8CAF4; -.
DR MaxQB; Q8CAF4; -.
DR PaxDb; Q8CAF4; -.
DR PeptideAtlas; Q8CAF4; -.
DR PRIDE; Q8CAF4; -.
DR ProteomicsDB; 252893; -. [Q8CAF4-1]
DR ProteomicsDB; 252894; -. [Q8CAF4-3]
DR Antibodypedia; 33056; 67 antibodies from 13 providers.
DR Ensembl; ENSMUST00000037341; ENSMUSP00000040799; ENSMUSG00000039835. [Q8CAF4-1]
DR Ensembl; ENSMUST00000100054; ENSMUSP00000097631; ENSMUSG00000039835. [Q8CAF4-3]
DR GeneID; 215819; -.
DR KEGG; mmu:215819; -.
DR UCSC; uc007emi.1; mouse. [Q8CAF4-1]
DR UCSC; uc007emj.2; mouse. [Q8CAF4-3]
DR CTD; 57224; -.
DR MGI; MGI:106390; Nhsl1.
DR VEuPathDB; HostDB:ENSMUSG00000039835; -.
DR eggNOG; ENOG502QTFI; Eukaryota.
DR GeneTree; ENSGT00950000182963; -.
DR InParanoid; Q8CAF4; -.
DR OrthoDB; 60972at2759; -.
DR PhylomeDB; Q8CAF4; -.
DR TreeFam; TF333323; -.
DR BioGRID-ORCS; 215819; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Nhsl1; mouse.
DR PRO; PR:Q8CAF4; -.
DR Proteomes; UP000000589; Chromosome 10.
DR RNAct; Q8CAF4; protein.
DR Bgee; ENSMUSG00000039835; Expressed in epithelium of lens and 236 other tissues.
DR ExpressionAtlas; Q8CAF4; baseline and differential.
DR Genevisible; Q8CAF4; MM.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR InterPro; IPR024845; NHS_fam.
DR Pfam; PF15273; NHS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..1587
FT /note="NHS-like protein 1"
FT /id="PRO_0000341354"
FT REGION 140..163
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 176..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 431..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 549..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 661..687
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 705..767
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 789..1059
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1083..1534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1565..1587
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 183..202
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 431..449
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 450..470
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 705..720
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 730..767
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 789..804
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 834..858
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 884..915
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 921..975
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 993..1023
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1108..1142
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1176..1190
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1257..1304
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1326..1372
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1384..1412
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1447..1464
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1484..1516
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 24
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 197
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5SYE7"
FT MOD_RES 328
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 563
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 629
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1079
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 1157
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5SYE7"
FT MOD_RES 1218
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q5SYE7"
FT MOD_RES 1373
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 1375
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 1379
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..68
FT /note="MKKDGSSGSFGIKASPGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGHQV
FT EADDENWIYRTQPRKA -> MVVFINAKIKSFFKLFKKKT (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_034261"
FT VAR_SEQ 224
FT /note="T -> TGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKEL
FT (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_034263"
FT CONFLICT 125
FT /note="Q -> R (in Ref. 3; AAH30842)"
FT /evidence="ECO:0000305"
FT CONFLICT 1555
FT /note="S -> G (in Ref. 3; AAH61949 and 4; BAD90251)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1587 AA; 169419 MW; 5E63EA1AEAAE412F CRC64;
MKKDGSSGSF GIKASPGSLS RAVSWINFSS LSRQTKRLFR SDGELSVCGH QVEADDENWI
YRTQPRKAVS NLDEESRWTV HYTAPWHQQE NVFLPATRPP CVEDLHRQAK LNLKSVLREC
DKLRQDGCRS SQYYSQGPTF AAGSSPCDDY QDEDTEADRK CSLSSSEEER FIGIRRPKTP
TSGDFSDLHT QTNWTKSLPL PTPEEKTRQQ AQTVQADVVP INITASATGQ DDDGSAHSLY
VPDHYSTLGR LDSYRSTGQC LETRDTSCQT EDVKVIPPSM RRIRAHKGVG VAAQMSHLSG
SSGNMSVLSD SAGVVFPSRL SNDTGFHSLP RTGPRASTYS LEGRMGALGS TEDTDDTSPY
QGGSLQGHEN FAHLGGASST GMLSRPKSQQ LRFLESPACV VSPHAAYSTS VIPNATLLSS
SEVIVIHTAQ SAGQLDSRTP GSSSYSKIKP RDRPTPRCSV KDDHQSPRHH WNEGHLIHSR
ALASSVPGAT TLLSLHDSEV SLNAPANREN GSQAILYHCR NNPSFPDHPS DVDGKSECSY
SGDRGCGSSE PWEYKTSSNG RASPLKPHLA TPGCSTPTSN VSSCSLDQTS LKGDTRSLCS
EDHDGYYTTT HEAGNLYTLS DGLGNPRHSM VNVFDGRAQR SQGDQAAHQD KILSRNISLK
KAKKPPLPPS RTDSLRRIPK KNNQTNGQVL NESLIASLQH SLQLSLPGKG GSSPSQSPCS
DFEEPWLPRS RSQSIVSEGS SLTSTTTPNV YSLCGVTPSQ SDTSSVKSEY TDPWGYYIDY
TSLQEDPGNP TGGCSANTEA ATGNGPVRHI QEGSRVPVPQ VPGCSVRPKI ASPEKSQRVT
SPSSGYSSQS NTPTALTPVP VFLKSMSPAN GKGKAKPKVP ERKSSLISSM SISSSSTSLS
SNTSTEGSGT MKKLDTTLAS ALAPPPPPLP PLPSPCLADK SPFLPPPPPL ADCSEGSPLP
PSPMFPPPPP EALVPFCSPT DGCLSPSPTA VSPSLPRSLP PVPAPPPFLP SSEPPPAPPL
DPKLMKENRP FFKNSSQSES SREALRRPAN KEEGCRPPMP LITTEALQMV QLRPVRKNSG
AGAVLFSEPS AQEQRTPTAP QYHLKPSAFL KSRNSINEME SESQAASVTS SLPMPAKSQS
QGDHDSAVER GGLPSCSDGA PGPGPSLRTT LLPDSSPSRK PPPISKKPKL FLVVPPPQRD
FTAEPTENGS EAFPGVPSPT RAEGEAVRSQ EEKSSPASRA GSHATAPTPG SPALEPGTAG
SLSSSIVEAN VPMVQPNTSP GPTQEESGEN SVDGERNAKS CLSQQGREAG LLEPNTAASS
SDPVDVSKEE GSDEVLTPTK PRTTEDLFAA IHRSKRKVLG RKDSEDDHTR NHSPSPPVTP
TSAAPNLASP KQVGSIQRSI KKSTTSSDNF KALLLKKGSR SDTSARMSAA EMLKSTDPRF
QRSRSEPSAD SPDSPSSCSP NKNRRAQEEW AKNEGLMPRS LSFSGPRYSR SRTPPSAASS
RYSMRNRIQS SPMTVISEGE GEPAEPADNK ARRALDATRV CSLDRLTGQE MDQASLLCSE
EPASVDGIGR AEGNGPSEQC GGTEQKS
