NHSL2_HUMAN
ID NHSL2_HUMAN Reviewed; 1225 AA.
AC Q5HYW2; A0A0J9YY34; B2RN94;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-APR-2019, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=NHS-like protein 2 {ECO:0000305};
GN Name=NHSL2 {ECO:0000312|HGNC:HGNC:33737};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15772651; DOI=10.1038/nature03440;
RA Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D.,
RA Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L.,
RA Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C.,
RA Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A.,
RA Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P.,
RA Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D.,
RA Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D.,
RA Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L.,
RA Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P.,
RA Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G.,
RA Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J.,
RA Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D.,
RA Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L.,
RA Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z.,
RA Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S.,
RA Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S.,
RA Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O.,
RA Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H.,
RA Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T.,
RA Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L.,
RA Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R.,
RA Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y.,
RA Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K.,
RA Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J.,
RA Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L.,
RA Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S.,
RA Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A.,
RA Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L.,
RA Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D.,
RA Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H.,
RA McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S.,
RA Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C.,
RA Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S.,
RA Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V.,
RA Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K.,
RA Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K.,
RA Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D.,
RA Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R.,
RA Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B.,
RA Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C.,
RA d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q.,
RA Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N.,
RA Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A.,
RA Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J.,
RA Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A.,
RA Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F.,
RA Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L.,
RA Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S.,
RA Rogers J., Bentley D.R.;
RT "The DNA sequence of the human X chromosome.";
RL Nature 434:325-337(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
CC -!- INTERACTION:
CC Q5HYW2; Q8IZP0-5: ABI1; NbExp=3; IntAct=EBI-2859639, EBI-11743294;
CC Q5HYW2; Q9NYB9-2: ABI2; NbExp=9; IntAct=EBI-2859639, EBI-11096309;
CC Q5HYW2; Q9P2A4: ABI3; NbExp=3; IntAct=EBI-2859639, EBI-742038;
CC Q5HYW2; Q4LE39-3: ARID4B; NbExp=3; IntAct=EBI-2859639, EBI-11957452;
CC Q5HYW2; P54253: ATXN1; NbExp=3; IntAct=EBI-2859639, EBI-930964;
CC Q5HYW2; Q9Y297: BTRC; NbExp=3; IntAct=EBI-2859639, EBI-307461;
CC Q5HYW2; O00299: CLIC1; NbExp=3; IntAct=EBI-2859639, EBI-347404;
CC Q5HYW2; Q14247-3: CTTN; NbExp=3; IntAct=EBI-2859639, EBI-12748199;
CC Q5HYW2; P63172: DYNLT1; NbExp=3; IntAct=EBI-2859639, EBI-1176455;
CC Q5HYW2; Q9H8Y8: GORASP2; NbExp=3; IntAct=EBI-2859639, EBI-739467;
CC Q5HYW2; A0A024R8L2: hCG_1987119; NbExp=3; IntAct=EBI-2859639, EBI-14103818;
CC Q5HYW2; O75031: HSF2BP; NbExp=3; IntAct=EBI-2859639, EBI-7116203;
CC Q5HYW2; Q0VD86: INCA1; NbExp=3; IntAct=EBI-2859639, EBI-6509505;
CC Q5HYW2; P45984: MAPK9; NbExp=5; IntAct=EBI-2859639, EBI-713568;
CC Q5HYW2; Q5JR59-3: MTUS2; NbExp=3; IntAct=EBI-2859639, EBI-11522433;
CC Q5HYW2; P16333: NCK1; NbExp=3; IntAct=EBI-2859639, EBI-389883;
CC Q5HYW2; O43639: NCK2; NbExp=6; IntAct=EBI-2859639, EBI-713635;
CC Q5HYW2; Q8WWB5: PIH1D2; NbExp=3; IntAct=EBI-2859639, EBI-10232538;
CC Q5HYW2; P53350: PLK1; NbExp=3; IntAct=EBI-2859639, EBI-476768;
CC Q5HYW2; Q96KQ4: PPP1R13B; NbExp=3; IntAct=EBI-2859639, EBI-1105153;
CC Q5HYW2; O60504: SORBS3; NbExp=3; IntAct=EBI-2859639, EBI-741237;
CC Q5HYW2; Q12800: TFCP2; NbExp=3; IntAct=EBI-2859639, EBI-717422;
CC Q5HYW2; Q05BL1: TP53BP2; NbExp=3; IntAct=EBI-2859639, EBI-11952721;
CC Q5HYW2; Q12933: TRAF2; NbExp=3; IntAct=EBI-2859639, EBI-355744;
CC Q5HYW2; P61981: YWHAG; NbExp=3; IntAct=EBI-2859639, EBI-359832;
CC Q5HYW2; Q04917: YWHAH; NbExp=3; IntAct=EBI-2859639, EBI-306940;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5HYW2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5HYW2-2; Sequence=VSP_060098, VSP_060099;
CC -!- SIMILARITY: Belongs to the NHS family. {ECO:0000305}.
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DR EMBL; BX119917; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL929401; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC136756; AAI36757.1; -; mRNA.
DR CCDS; CCDS87759.1; -. [Q5HYW2-1]
DR RefSeq; NP_001013649.2; NM_001013627.2. [Q5HYW2-1]
DR AlphaFoldDB; Q5HYW2; -.
DR IntAct; Q5HYW2; 42.
DR STRING; 9606.ENSP00000424079; -.
DR iPTMnet; Q5HYW2; -.
DR PhosphoSitePlus; Q5HYW2; -.
DR BioMuta; NHSL2; -.
DR DMDM; 74741562; -.
DR EPD; Q5HYW2; -.
DR jPOST; Q5HYW2; -.
DR MassIVE; Q5HYW2; -.
DR PaxDb; Q5HYW2; -.
DR PeptideAtlas; Q5HYW2; -.
DR PRIDE; Q5HYW2; -.
DR ProteomicsDB; 62962; -.
DR Antibodypedia; 35138; 14 antibodies from 6 providers.
DR DNASU; 340527; -.
DR Ensembl; ENST00000510661.2; ENSP00000424079.2; ENSG00000204131.10. [Q5HYW2-2]
DR Ensembl; ENST00000633930.2; ENSP00000488668.1; ENSG00000204131.10. [Q5HYW2-1]
DR GeneID; 340527; -.
DR KEGG; hsa:340527; -.
DR MANE-Select; ENST00000633930.2; ENSP00000488668.1; NM_001013627.3; NP_001013649.2.
DR UCSC; uc004eak.1; human. [Q5HYW2-1]
DR CTD; 340527; -.
DR DisGeNET; 340527; -.
DR GeneCards; NHSL2; -.
DR HGNC; HGNC:33737; NHSL2.
DR HPA; ENSG00000204131; Low tissue specificity.
DR neXtProt; NX_Q5HYW2; -.
DR OpenTargets; ENSG00000204131; -.
DR VEuPathDB; HostDB:ENSG00000204131; -.
DR eggNOG; ENOG502QQ7S; Eukaryota.
DR GeneTree; ENSGT00950000182963; -.
DR InParanoid; Q5HYW2; -.
DR OMA; EPEDPHQ; -.
DR OrthoDB; 82879at2759; -.
DR PhylomeDB; Q5HYW2; -.
DR PathwayCommons; Q5HYW2; -.
DR SignaLink; Q5HYW2; -.
DR BioGRID-ORCS; 340527; 6 hits in 216 CRISPR screens.
DR ChiTaRS; NHSL2; human.
DR GenomeRNAi; 340527; -.
DR Pharos; Q5HYW2; Tdark.
DR PRO; PR:Q5HYW2; -.
DR Proteomes; UP000005640; Chromosome X.
DR RNAct; Q5HYW2; protein.
DR Bgee; ENSG00000204131; Expressed in sural nerve and 137 other tissues.
DR ExpressionAtlas; Q5HYW2; baseline and differential.
DR Genevisible; Q5HYW2; HS.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR InterPro; IPR024845; NHS_fam.
DR Pfam; PF15273; NHS; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..1225
FT /note="NHS-like protein 2"
FT /id="PRO_0000341355"
FT REGION 161..180
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 193..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 291..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 466..510
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 543..632
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 670..766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 812..1009
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1042..1093
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1128..1203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..176
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 291..318
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 670..695
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 706..736
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 812..832
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 852..873
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 874..888
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 935..970
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1053..1071
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1140..1169
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 500
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:B1AXH1"
FT MOD_RES 576
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:B1AXH1"
FT MOD_RES 691
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:B1AXH1"
FT MOD_RES 1054
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:B1AXH1"
FT VAR_SEQ 1..366
FT /note="Missing (in isoform 2)"
FT /id="VSP_060098"
FT VAR_SEQ 1076..1225
FT /note="Missing (in isoform 2)"
FT /id="VSP_060099"
FT VARIANT 901
FT /note="T -> I (in dbSNP:rs7061150)"
FT /id="VAR_044057"
SQ SEQUENCE 1225 AA; 133286 MW; 84681065AD4F7D5E CRC64;
MPFYRRTVVP QRLCPRNPPQ QLAELRDVSH LAALSLLRQL ADLCGHSLAL LEDLEGHLLA
LGRRTDSLYR RTVRLRRRLP CRLLGPEEDE EELAAANSGR ENATATAHSR SSWRQPVNVF
LSSGRPPSVE ELLREAQLNL QSLLQEEYEE QYSEARLVGQ TFRSSDEATK PTPNPRPQSA
RRLEFILMPT KRQLSEDETT TQGVRAPEAS LSLSTTADKQ TAWNSLFPLP ILEEKRWPQL
CSTQSDIVPI NISGQQFDKH ASLRHSLFNT ETAVNPKSTL RRRRTIIGFS NFSQRDQGHS
NSPAGSVAHS TTSDIRPSHS VPEGVHGRVA VGQDARFPSL TSPVLRTPSS EPDEPHQARS
GPNPPGMESM GMVYSVPSSC NGPTESTFST SWKGDAFTYM TPSATSQSNQ VNENGKNPSC
GNSWVSLNKV PPLVPKEAAT LLVARDNPAG CSGSAGYPER LIQQRHMPER PSKIGLLTSG
TSRLETGPGG ASRFRERSLS VPTDSGTTDV DYDEEQKANE ACALPFASTS SEGSNSADNI
ASLSAQQEAQ HRRQRSKSIS LRKAKKKPSP PTRSVSLVKD EPGLLPEGGS ALPKDQRPKS
LCLSLEHQGH HSSHPDAQGH PAIPNHKDPE STQFSHHWYL TDWKSGDTYQ SLSSSSTATG
TTVIECTQVQ GSSESLASPS TSRATTPSQL SIEVEAREIS SPGRPPGLMS PSSGYSSQSE
TPTPTVSMSL TLGHLPPPSS SVRVRPVVPE RKSSLPPTSP MEKFPKSRLS FDLPLTSSPN
LDLSGMSISI RSKTKVSRHH SETNFGVKLA QKTNPNQPIM PMVTQSDLRS VRLRSVSKSE
PEDDIESPEY AEEPRAEEVF TLPERKTKPP VAEKPPVARR PPSLVHKPPS VPEEYALTSP
TLAMPPRSSI QHARPLPQDS YTVVRKPKPS SFPDGRSPGE STAPSSLVFT PFASSSDAFF
SGTQQPPQGS VEDEGPKVRV LPERISLQSQ EEAEKKKGKI PPPVPKKPSV LYLPLTSPTA
QMEAYVAEPR LPLSPIITLE EDTKCPATGD DLQSLGQRVT STPQADSERE ASPLGSSVEP
GTEEKSLISD KTAEWIAEDD DDVFVASRTT EDLFTVIHRS KRKLLGWKEP GEAFVGGRTS
SHSPIKNTAE SPISESTATA GSGSSANLDA GRNDDFKALL QKKGSKATPR SRPSAAELLK
TTNPLARRII AQFSKDYETT DNPST
