NHX1_ORYSJ
ID NHX1_ORYSJ Reviewed; 535 AA.
AC Q9SXJ8; A0A5S6RC28; Q0D3T8; Q8H486;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Sodium/hydrogen exchanger 1 {ECO:0000303|PubMed:10395929};
DE AltName: Full=Na(+)/H(+) exchanger 1 {ECO:0000303|PubMed:10395929};
DE Short=OsNHX1 {ECO:0000303|PubMed:10395929};
GN Name=NHX1 {ECO:0000303|PubMed:10395929};
GN OrderedLocusNames=Os07g0666900 {ECO:0000312|EMBL:BAT03109.1},
GN LOC_Os07g47100 {ECO:0000305};
GN ORFNames=P0450A04.124 {ECO:0000312|EMBL:BAF22485.2};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION BY SALT.
RX PubMed=10395929; DOI=10.1016/s0167-4781(99)00065-2;
RA Fukuda A., Nakamura A., Tanaka Y.;
RT "Molecular cloning and expression of the Na+/H+ exchanger gene in Oryza
RT sativa.";
RL Biochim. Biophys. Acta 1446:149-155(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION BY SALT.
RX PubMed=14988485; DOI=10.1093/pcp/pch014;
RA Fukuda A., Nakamura A., Tagiri A., Tanaka H., Miyao A., Hirochika H.,
RA Tanaka Y.;
RT "Function, intracellular localization and the importance in salt tolerance
RT of a vacuolar Na(+)/H(+) antiporter from rice.";
RL Plant Cell Physiol. 45:146-159(2004).
CC -!- FUNCTION: Vacuolar antiporter that acts in low affinity electroneutral
CC exchange of protons H(+) for cations such as Na(+) or K(+) across
CC membranes (PubMed:14988485). Plays important roles in the transport of
CC Na(+) and K(+) accumulated in the cytoplasm into vacuoles, and is
CC involved in salt stress tolerance (PubMed:14988485).
CC {ECO:0000269|PubMed:14988485}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + Na(+)(in) = H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29419, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000269|PubMed:14988485};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + K(+)(in) = H(+)(in) + K(+)(out);
CC Xref=Rhea:RHEA:29467, ChEBI:CHEBI:15378, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000269|PubMed:14988485};
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:14988485};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- INDUCTION: Induced by NaCl in roots and shoots (PubMed:10395929,
CC PubMed:14988485). Induced by KCl in roots and shoots (PubMed:14988485).
CC {ECO:0000269|PubMed:10395929, ECO:0000269|PubMed:14988485}.
CC -!- MISCELLANEOUS: Plants overexpressing NHX1 exhibit improved tolerance to
CC salt stress. {ECO:0000269|PubMed:14988485}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF22485.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB021878; BAA83337.1; -; mRNA.
DR EMBL; AP004274; BAC20076.1; -; Genomic_DNA.
DR EMBL; AP008213; BAF22485.2; ALT_INIT; Genomic_DNA.
DR EMBL; AP014963; BAT03109.1; -; Genomic_DNA.
DR EMBL; AK064004; BAG88958.1; -; mRNA.
DR RefSeq; XP_015644575.1; XM_015789089.1.
DR AlphaFoldDB; Q9SXJ8; -.
DR SMR; Q9SXJ8; -.
DR STRING; 4530.OS07T0666900-01; -.
DR PaxDb; Q9SXJ8; -.
DR PRIDE; Q9SXJ8; -.
DR EnsemblPlants; Os07t0666900-01; Os07t0666900-01; Os07g0666900.
DR EnsemblPlants; Os07t0666900-02; Os07t0666900-02; Os07g0666900.
DR GeneID; 4344217; -.
DR Gramene; Os07t0666900-01; Os07t0666900-01; Os07g0666900.
DR Gramene; Os07t0666900-02; Os07t0666900-02; Os07g0666900.
DR KEGG; osa:4344217; -.
DR eggNOG; KOG1965; Eukaryota.
DR HOGENOM; CLU_005912_11_2_1; -.
DR InParanoid; Q9SXJ8; -.
DR OMA; FAYIGLH; -.
DR OrthoDB; 546232at2759; -.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR GO; GO:0098719; P:sodium ion import across plasma membrane; IBA:GO_Central.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR InterPro; IPR004709; NaH_exchanger.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR PRINTS; PR01084; NAHEXCHNGR.
DR TIGRFAMs; TIGR00840; b_cpa1; 1.
PE 2: Evidence at transcript level;
KW Antiport; Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; Sodium; Sodium transport; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..535
FT /note="Sodium/hydrogen exchanger 1"
FT /id="PRO_0000455449"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..46
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..75
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 97..114
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..137
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 159..173
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 195..218
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..264
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..304
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..344
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 366..381
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 382..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 403..415
FT /note="Vacuolar"
FT /evidence="ECO:0000305"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 437..535
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 452..478
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 535 AA; 59070 MW; 6BCADDB29B131976 CRC64;
MGMEVAAARL GALYTTSDYA SVVSINLFVA LLCACIVLGH LLEENRWVNE SITALIIGLC
TGVVILLMTK GKSSHLFVFS EDLFFIYLLP PIIFNAGFQV KKKQFFRNFM TITLFGAVGT
MISFFTISIA AIAIFSRMNI GTLDVGDFLA IGAIFSATDS VCTLQVLNQD ETPFLYSLVF
GEGVVNDATS IVLFNALQNF DLVHIDAAVV LKFLGNFFYL FLSSTFLGVF AGLLSAYIIK
KLYIGRHSTD REVALMMLMA YLSYMLAELL DLSGILTVFF CGIVMSHYTW HNVTESSRVT
TKHAFATLSF IAETFLFLYV GMDALDIEKW EFASDRPGKS IGISSILLGL VLIGRAAFVF
PLSFLSNLTK KAPNEKITWR QQVVIWWAGL MRGAVSIALA YNKFTRSGHT QLHGNAIMIT
STITVVLFST MVFGMMTKPL IRLLLPASGH PVTSEPSSPK SLHSPLLTSM QGSDLESTTN
IVRPSSLRML LTKPTHTVHY YWRKFDDALM RPMFGGRGFV PFSPGSPTEQ SHGGR
