NHX4_ARATH
ID NHX4_ARATH Reviewed; 529 AA.
AC Q8S397; Q9FJ63;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Sodium/hydrogen exchanger 4;
DE AltName: Full=Na(+)/H(+) exchanger 4;
DE Short=NHE-4;
GN Name=NHX4; OrderedLocusNames=At5g55470; ORFNames=MTE17.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Wassilewskija;
RX PubMed=12047628; DOI=10.1046/j.1365-313x.2002.01309.x;
RA Yokoi S., Quintero F.J., Cubero B., Ruiz M.T., Bressan R.A., Hasegawa P.M.,
RA Pardo J.M.;
RT "Differential expression and function of Arabidopsis thaliana NHX
RT Na(+)/H(+) antiporters in the salt stress response.";
RL Plant J. 30:529-539(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May act in low affinity electroneutral exchange of protons
CC for cations such as Na(+) or K(+) across membranes. May also exchange
CC Li(+) and Cs(+) with a lower affinity. {ECO:0000250|UniProtKB:Q68KI4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + Na(+)(in) = H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29419, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000250|UniProtKB:Q68KI4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + K(+)(in) = H(+)(in) + K(+)(out);
CC Xref=Rhea:RHEA:29467, ChEBI:CHEBI:15378, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000250|UniProtKB:Q68KI4};
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250|UniProtKB:Q68KI4};
CC Multi-pass membrane protein {ECO:0000255}. Note=Tonoplast.
CC -!- TISSUE SPECIFICITY: Expressed at very low levels in roots and shoots.
CC {ECO:0000269|PubMed:12047628}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. {ECO:0000305}.
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DR EMBL; AF490588; AAM08405.1; -; mRNA.
DR EMBL; AB015479; BAB08564.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96632.1; -; Genomic_DNA.
DR EMBL; AK221571; BAD95025.1; -; mRNA.
DR RefSeq; NP_200358.1; NM_124929.3.
DR AlphaFoldDB; Q8S397; -.
DR SMR; Q8S397; -.
DR BioGRID; 20884; 9.
DR IntAct; Q8S397; 4.
DR STRING; 3702.AT5G55470.1; -.
DR TCDB; 2.A.36.5.8; the monovalent cation:proton antiporter-1 (cpa1) family.
DR iPTMnet; Q8S397; -.
DR PaxDb; Q8S397; -.
DR PRIDE; Q8S397; -.
DR ProteomicsDB; 236825; -.
DR EnsemblPlants; AT5G55470.1; AT5G55470.1; AT5G55470.
DR GeneID; 835640; -.
DR Gramene; AT5G55470.1; AT5G55470.1; AT5G55470.
DR KEGG; ath:AT5G55470; -.
DR Araport; AT5G55470; -.
DR TAIR; locus:2173972; AT5G55470.
DR eggNOG; KOG1965; Eukaryota.
DR HOGENOM; CLU_005912_11_2_1; -.
DR InParanoid; Q8S397; -.
DR OMA; ETVVMWW; -.
DR OrthoDB; 546232at2759; -.
DR PhylomeDB; Q8S397; -.
DR PRO; PR:Q8S397; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8S397; baseline and differential.
DR Genevisible; Q8S397; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0015081; F:sodium ion transmembrane transporter activity; ISS:TAIR.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IDA:TAIR.
DR GO; GO:0055075; P:potassium ion homeostasis; IMP:TAIR.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR GO; GO:0098719; P:sodium ion import across plasma membrane; IBA:GO_Central.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR InterPro; IPR004709; NaH_exchanger.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR PRINTS; PR01084; NAHEXCHNGR.
DR TIGRFAMs; TIGR00840; b_cpa1; 1.
PE 2: Evidence at transcript level;
KW Antiport; Glycoprotein; Ion transport; Membrane; Potassium;
KW Potassium transport; Reference proteome; Sodium; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..529
FT /note="Sodium/hydrogen exchanger 4"
FT /id="PRO_0000052375"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..45
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..73
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT INTRAMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..112
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..171
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..214
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..250
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 268..273
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..301
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..322
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 323..342
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..380
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 402..415
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 416..436
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 437..529
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT CARBOHYD 374
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 8
FT /note="F -> C (in Ref. 1; AAM08405)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 529 AA; 58872 MW; 8A6BD42E822F90C1 CRC64;
MSIGLTEFVT NKLAAEHPQV IPISVFIAIL CLCLVIGHLL EENRWVNESI TAILVGAASG
TVILLISKGK SSHILVFDEE LFFIYLLPPI IFNAGFQVKK KKFFHNFLTI MSFGVIGVFI
STVIISFGTW WLFPKLGFKG LSARDYLAIG TIFSSTDTVC TLQILHQDET PLLYSLVFGE
GVVNDATSVV LFNAVQKIQF ESLTGWTALQ VFGNFLYLFS TSTLLGIGVG LITSFVLKTL
YFGRHSTTRE LAIMVLMAYL SYMLAELFSL SGILTVFFCG VLMSHYASYN VTESSRITSR
HVFAMLSFIA ETFIFLYVGT DALDFTKWKT SSLSFGGTLG VSGVITALVL LGRAAFVFPL
SVLTNFMNRH TERNESITFK HQVIIWWAGL MRGAVSIALA FKQFTYSGVT LDPVNAAMVT
NTTIVVLFTT LVFGFLTKPL VNYLLPQDAS HNTGNRGKRT EPGSPKEDAT LPLLSFDESA
STNFNRAKDS ISLLMEQPVY TIHRYWRKFD DTYMRPIFGG PRRENQPEC
