NHX5_ARATH
ID NHX5_ARATH Reviewed; 521 AA.
AC Q8S396; Q0WWC0; Q9SLJ7;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Sodium/hydrogen exchanger 5;
DE AltName: Full=Na(+)/H(+) exchanger 5;
DE Short=NHE-5;
GN Name=NHX5; OrderedLocusNames=At1g54370; ORFNames=F20D21.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 5-521, FUNCTION, TISSUE SPECIFICITY, AND
RP INDUCTION.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=12047628; DOI=10.1046/j.1365-313x.2002.01309.x;
RA Yokoi S., Quintero F.J., Cubero B., Ruiz M.T., Bressan R.A., Hasegawa P.M.,
RA Pardo J.M.;
RT "Differential expression and function of Arabidopsis thaliana NHX
RT Na(+)/H(+) antiporters in the salt stress response.";
RL Plant J. 30:529-539(2002).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=21278129; DOI=10.1105/tpc.110.079426;
RA Bassil E., Ohto M.A., Esumi T., Tajima H., Zhu Z., Cagnac O., Belmonte M.,
RA Peleg Z., Yamaguchi T., Blumwald E.;
RT "The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are
RT endosome associated and necessary for plant growth and development.";
RL Plant Cell 23:224-239(2011).
CC -!- FUNCTION: Involved in trafficking to the vacuole (PubMed:21278129).
CC Required for cell proliferation and cell expansion, but not for cell
CC differentiation (PubMed:21278129). Acts in low affinity electroneutral
CC exchange of protons for cations such as Na(+) or K(+) across membranes
CC (PubMed:12047628). May also exchange Li(+) and Cs(+) with a lower
CC affinity (By similarity). {ECO:0000250|UniProtKB:Q68KI4,
CC ECO:0000269|PubMed:12047628, ECO:0000269|PubMed:21278129}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + Na(+)(in) = H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29419, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000250|UniProtKB:Q68KI4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + K(+)(in) = H(+)(in) + K(+)(out);
CC Xref=Rhea:RHEA:29467, ChEBI:CHEBI:15378, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000250|UniProtKB:Q68KI4};
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000269|PubMed:21278129};
CC Multi-pass membrane protein {ECO:0000269|PubMed:21278129}. Golgi
CC apparatus, trans-Golgi network membrane {ECO:0000269|PubMed:21278129};
CC Multi-pass membrane protein {ECO:0000269|PubMed:21278129}. Golgi
CC apparatus, Golgi stack membrane {ECO:0000269|PubMed:21278129}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:21278129}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers and
CC siliques. Detected at low levels in roots and shoots.
CC {ECO:0000269|PubMed:12047628, ECO:0000269|PubMed:21278129}.
CC -!- INDUCTION: Induced by NaCl in a ABA-independent manner.
CC {ECO:0000269|PubMed:12047628}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype; due to redundancy with
CC NHX6. Nhx5 and nhx6 double mutant has a slower development, is
CC drastically smaller and is salt sensitive.
CC {ECO:0000269|PubMed:21278129}.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD25617.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC005287; AAD25617.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33089.1; -; Genomic_DNA.
DR EMBL; AK226435; BAE98578.1; -; mRNA.
DR EMBL; AF490589; AAM08406.1; -; mRNA.
DR PIR; D96585; D96585.
DR RefSeq; NP_175839.2; NM_104315.5.
DR AlphaFoldDB; Q8S396; -.
DR SMR; Q8S396; -.
DR BioGRID; 27103; 4.
DR STRING; 3702.AT1G54370.1; -.
DR TCDB; 2.A.36.1.20; the monovalent cation:proton antiporter-1 (cpa1) family.
DR iPTMnet; Q8S396; -.
DR PaxDb; Q8S396; -.
DR PRIDE; Q8S396; -.
DR ProteomicsDB; 250562; -.
DR EnsemblPlants; AT1G54370.1; AT1G54370.1; AT1G54370.
DR GeneID; 841878; -.
DR Gramene; AT1G54370.1; AT1G54370.1; AT1G54370.
DR KEGG; ath:AT1G54370; -.
DR Araport; AT1G54370; -.
DR TAIR; locus:2020068; AT1G54370.
DR eggNOG; KOG1965; Eukaryota.
DR HOGENOM; CLU_005912_11_1_1; -.
DR InParanoid; Q8S396; -.
DR OMA; SYRWPIG; -.
DR OrthoDB; 559433at2759; -.
DR PRO; PR:Q8S396; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8S396; baseline and differential.
DR Genevisible; Q8S396; AT.
DR GO; GO:0005768; C:endosome; IDA:TAIR.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR GO; GO:0098719; P:sodium ion import across plasma membrane; IBA:GO_Central.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR InterPro; IPR004709; NaH_exchanger.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR PRINTS; PR01084; NAHEXCHNGR.
DR TIGRFAMs; TIGR00840; b_cpa1; 1.
PE 2: Evidence at transcript level;
KW Antiport; Endosome; Glycoprotein; Golgi apparatus; Ion transport; Membrane;
KW Potassium; Potassium transport; Reference proteome; Sodium;
KW Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..521
FT /note="Sodium/hydrogen exchanger 5"
FT /id="PRO_0000052376"
FT TOPO_DOM 1..23
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 45..48
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT INTRAMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..115
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 137..141
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT INTRAMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT INTRAMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..222
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..271
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 293..310
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 332..340
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 362..382
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..402
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 403..411
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 433..521
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT REGION 453..480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 299
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 521 AA; 57335 MW; 969FFBD981F5E1E2 CRC64;
MEEVMISPVE HDPQGQVKQQ QAAGVGILLQ IMMLVLSFVL GHVLRRHRFH YLPEASGSLL
IGLIVGILAN ISDTETSIRT WFNFHEEFFF LFLLPPIIFQ SGFSLQPKPF FSNFGAIVTF
AIIGTFVASV VTGGLVYLGG SMYLMYKLPF VECLMFGALI SATDPVTVLS IFQDVGTDVN
LYALVFGESV LNDAMAISLY RTMSLVNRQS SSGEHFFMVV IRFFETFAGS MSAGVGVGFT
SALLFKYAGL DTENLQNLEC CLFVLFPYFS YMLAEGVGLS GIVSILFTGI VMKRYTFSNL
SEASQSFVSS FFHLISSLAE TFTFIYMGFD IAMEQHSWSH VGFILFSILF IGVARAVNVF
GCAYLVNLFR QENQKIPMKH QKALWYSGLR GAMAFALALQ SLHDLPEGHG QIIFTATTTI
VVVTVLLIGG STGKMLEALE VVGDDLDDSM SEGFEESDHQ YVPPPFSIGA SSDEDTSSSG
SRFKMKLKEF HKTTTSFTAL DKNFLTPFFT TNSGDGDGDG E
