NHX7_ARATH
ID NHX7_ARATH Reviewed; 1146 AA.
AC Q9LKW9; Q9ZPS6;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 139.
DE RecName: Full=Sodium/hydrogen exchanger 7;
DE AltName: Full=Na(+)/H(+) exchanger 7;
DE Short=NHE-7;
DE AltName: Full=Protein SALT OVERLY SENSITIVE 1;
GN Name=NHX7; Synonyms=SOS1; OrderedLocusNames=At2g01980; ORFNames=F14H20.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION,
RP AND MUTAGENESIS OF GLY-136; ARG-365; GLY-777 AND GLY-784.
RX PubMed=10823923; DOI=10.1073/pnas.120170197;
RA Shi H., Ishitani M., Kim C., Zhu J.-K.;
RT "The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative
RT Na(+)/H(+) antiporter.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:6896-6901(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF ARG-365.
RX PubMed=12239394; DOI=10.2307/3870339;
RA Wu S.-J., Ding L., Zhu J.-K.;
RT "SOS1, a genetic locus essential for salt tolerance and potassium
RT acquisition.";
RL Plant Cell 8:617-627(1996).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=11884687; DOI=10.1105/tpc.010371;
RA Shi H., Quintero F.J., Pardo J.M., Zhu J.-K.;
RT "The putative plasma membrane Na(+)/H(+) antiporter SOS1 controls long-
RT distance Na(+) transport in plants.";
RL Plant Cell 14:465-477(2002).
RN [7]
RP FUNCTION, AND REGULATION.
RX PubMed=12034882; DOI=10.1073/pnas.122224699;
RA Qiu Q.-S., Guo Y., Dietrich M.A., Schumaker K.S., Zhu J.-K.;
RT "Regulation of SOS1, a plasma membrane Na(+)/H(+) exchanger in Arabidopsis
RT thaliana, by SOS2 and SOS3.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:8436-8441(2002).
RN [8]
RP PHOSPHORYLATION, INTERACTION WITH CIPK24/SOS2 AND CBL4/SOS3, AND
RP REGULATION.
RX PubMed=12070350; DOI=10.1073/pnas.132092099;
RA Quintero F.J., Ohta M., Shi H., Zhu J.-K., Pardo J.M.;
RT "Reconstitution in yeast of the Arabidopsis SOS signaling pathway for Na(+)
RT homeostasis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:9061-9066(2002).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. La-0;
RX PubMed=14506206; DOI=10.1074/mcp.t300006-mcp200;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Large-scale analysis of in vivo phosphorylated membrane proteins by
RT immobilized metal ion affinity chromatography and mass spectrometry.";
RL Mol. Cell. Proteomics 2:1234-1243(2003).
RN [10]
RP FUNCTION.
RX PubMed=12469134; DOI=10.1038/nbt766;
RA Shi H., Lee B.-H., Wu S.-J., Zhu J.-K.;
RT "Overexpression of a plasma membrane Na(+)/H(+) antiporter gene improves
RT salt tolerance in Arabidopsis thaliana.";
RL Nat. Biotechnol. 21:81-85(2003).
RN [11]
RP FUNCTION, SUBCELLULAR LOCATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=12805632; DOI=10.1104/pp.102.010421;
RA Qiu Q.-S., Barkla B.J., Vera-Estrella R., Zhu J.-K., Schumaker K.S.;
RT "Na(+)/H(+) exchange activity in the plasma membrane of Arabidopsis.";
RL Plant Physiol. 132:1041-1052(2003).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=15308754; DOI=10.1105/tpc.104.023150;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Phosphoproteomics of the Arabidopsis plasma membrane and a new
RT phosphorylation site database.";
RL Plant Cell 16:2394-2405(2004).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17869214; DOI=10.1016/j.bbrc.2007.08.177;
RA Hem S., Rofidal V., Sommerer N., Rossignol M.;
RT "Novel subsets of the Arabidopsis plasmalemma phosphoproteome identify
RT phosphorylation sites in secondary active transporters.";
RL Biochem. Biophys. Res. Commun. 363:375-380(2007).
CC -!- FUNCTION: Acts in electroneutral exchange of protons for cations such
CC as Na(+) or Li(+) across plasma membrane. Involved in Na(+) and K(+)
CC homeostasis. Required for cytoplasmic Na(+) and Li(+) detoxification by
CC secreting them from the cytoplasm to the extracellular space. Regulates
CC Na(+) content of the xylem sap. {ECO:0000269|PubMed:10823923,
CC ECO:0000269|PubMed:11884687, ECO:0000269|PubMed:12034882,
CC ECO:0000269|PubMed:12239394, ECO:0000269|PubMed:12469134,
CC ECO:0000269|PubMed:12805632}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + Na(+)(in) = H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29419, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000269|PubMed:12239394};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + K(+)(in) = H(+)(in) + K(+)(out);
CC Xref=Rhea:RHEA:29467, ChEBI:CHEBI:15378, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000269|PubMed:12239394};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=22.8 mM for Na(+) {ECO:0000269|PubMed:12805632};
CC -!- SUBUNIT: Interacts with CIPK24/SOS2 and CBL4/SOS3.
CC {ECO:0000269|PubMed:12070350}.
CC -!- INTERACTION:
CC Q9LKW9; Q9LDI3: CIPK24; NbExp=4; IntAct=EBI-2368285, EBI-537551;
CC Q9LKW9; Q8RY59: RCD1; NbExp=5; IntAct=EBI-2368285, EBI-2118043;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11884687,
CC ECO:0000269|PubMed:12805632}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:11884687, ECO:0000269|PubMed:12805632}.
CC -!- TISSUE SPECIFICITY: More expressed in roots than in shoots. Mostly
CC localized in parenchyma cells at the xylem/symplast boundary in roots,
CC hypocotyls, stems and leaves. Also present in root tips epidermal
CC cells. {ECO:0000269|PubMed:10823923, ECO:0000269|PubMed:11884687}.
CC -!- INDUCTION: By Na(+), in a SOS signaling pathway-dependent manner.
CC {ECO:0000269|PubMed:10823923, ECO:0000269|PubMed:11884687}.
CC -!- PTM: Phosphorylated by CIPK24/SOS2 in complex with CBL4/SOS3.
CC {ECO:0000269|PubMed:12070350}.
CC -!- MISCELLANEOUS: Positively regulated by the salt overly sensitive (SOS)
CC signaling pathway that involves the kinase CIPK24/SOS2 and the calcium
CC sensor CBL4/SOS3.
CC -!- MISCELLANEOUS: Transgenic plants that overexpress NHX7 have enhanced
CC capability to grow on high saline soils.
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD20091.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF256224; AAF76139.1; -; Genomic_DNA.
DR EMBL; AC006532; AAD20091.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC05529.1; -; Genomic_DNA.
DR EMBL; AY062746; AAL32824.1; -; mRNA.
DR PIR; E84431; E84431.
DR RefSeq; NP_178307.2; NM_126259.4.
DR AlphaFoldDB; Q9LKW9; -.
DR SMR; Q9LKW9; -.
DR BioGRID; 132; 3.
DR DIP; DIP-52883N; -.
DR IntAct; Q9LKW9; 2.
DR STRING; 3702.AT2G01980.1; -.
DR TCDB; 2.A.36.7.6; the monovalent cation:proton antiporter-1 (cpa1) family.
DR iPTMnet; Q9LKW9; -.
DR PaxDb; Q9LKW9; -.
DR PRIDE; Q9LKW9; -.
DR ProteomicsDB; 249438; -.
DR EnsemblPlants; AT2G01980.1; AT2G01980.1; AT2G01980.
DR GeneID; 814729; -.
DR Gramene; AT2G01980.1; AT2G01980.1; AT2G01980.
DR KEGG; ath:AT2G01980; -.
DR Araport; AT2G01980; -.
DR TAIR; locus:2041100; AT2G01980.
DR eggNOG; KOG1965; Eukaryota.
DR HOGENOM; CLU_008625_0_0_1; -.
DR InParanoid; Q9LKW9; -.
DR OMA; DMITSHP; -.
DR OrthoDB; 99426at2759; -.
DR PhylomeDB; Q9LKW9; -.
DR PRO; PR:Q9LKW9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9LKW9; baseline and differential.
DR Genevisible; Q9LKW9; AT.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IMP:TAIR.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR GO; GO:2000377; P:regulation of reactive oxygen species metabolic process; IMP:TAIR.
DR GO; GO:0042542; P:response to hydrogen peroxide; IMP:TAIR.
DR GO; GO:0006979; P:response to oxidative stress; IMP:TAIR.
DR GO; GO:0000302; P:response to reactive oxygen species; IEP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR GO; GO:0098719; P:sodium ion import across plasma membrane; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; IMP:TAIR.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR InterPro; IPR018490; cNMP-bd-like.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell membrane; Ion transport; Membrane; Phosphoprotein;
KW Potassium; Potassium transport; Reference proteome; Sodium;
KW Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1146
FT /note="Sodium/hydrogen exchanger 7"
FT /id="PRO_0000052378"
FT TOPO_DOM 1..28
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 29..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 50..58
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..99
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..127
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..159
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..191
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 213..227
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..253
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 274..278
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..313
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 335..352
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374..387
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 388..408
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 409..420
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 442..1146
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 981..1001
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1102..1128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 136
FT /note="G->E: In sos1-12; hypersensitivity to Na(+) and
FT Li(+)."
FT /evidence="ECO:0000269|PubMed:10823923"
FT MUTAGEN 365
FT /note="R->C: In sos1-3; hypersensitivity to Na(+) and
FT Li(+)."
FT /evidence="ECO:0000269|PubMed:10823923,
FT ECO:0000269|PubMed:12239394"
FT MUTAGEN 777
FT /note="G->E: In sos1-8; hypersensitivity to Na(+) and
FT Li(+)."
FT /evidence="ECO:0000269|PubMed:10823923"
FT MUTAGEN 784
FT /note="G->D: In sos1-9; hypersensitivity to Na(+) and
FT Li(+)."
FT /evidence="ECO:0000269|PubMed:10823923"
SQ SEQUENCE 1146 AA; 127188 MW; DAEA315426F8AE7A CRC64;
MTTVIDATMA YRFLEEATDS SSSSSSSKLE SSPVDAVLFV GMSLVLGIAS RHLLRGTRVP
YTVALLVIGI ALGSLEYGAK HNLGKIGHGI RIWNEIDPEL LLAVFLPALL FESSFSMEVH
QIKRCLGQMV LLAVPGVLIS TACLGSLVKV TFPYEWDWKT SLLLGGLLSA TDPVAVVALL
KELGASKKLS TIIEGESLMN DGTAIVVFQL FLKMAMGQNS DWSSIIKFLL KVALGAVGIG
LAFGIASVIW LKFIFNDTVI EITLTIAVSY FAYYTAQEWA GASGVLTVMT LGMFYAAFAR
TAFKGDSQKS LHHFWEMVAY IANTLIFILS GVVIAEGILD SDKIAYQGNS WRFLFLLYVY
IQLSRVVVVG VLYPLLCRFG YGLDWKESII LVWSGLRGAV ALALSLSVKQ SSGNSHISKE
TGTLFLFFTG GIVFLTLIVN GSTTQFVLRL LRMDILPAPK KRILEYTKYE MLNKALRAFQ
DLGDDEELGP ADWPTVESYI SSLKGSEGEL VHHPHNGSKI GSLDPKSLKD IRMRFLNGVQ
ATYWEMLDEG RISEVTANIL MQSVDEALDQ VSTTLCDWRG LKPHVNFPNY YNFLHSKVVP
RKLVTYFAVE RLESACYISA AFLRAHTIAR QQLYDFLGES NIGSIVINES EKEGEEAKKF
LEKVRSSFPQ VLRVVKTKQV TYSVLNHLLG YIENLEKVGL LEEKEIAHLH DAVQTGLKKL
LRNPPIVKLP KLSDMITSHP LSVALPPAFC EPLKHSKKEP MKLRGVTLYK EGSKPTGVWL
IFDGIVKWKS KILSNNHSLH PTFSHGSTLG LYEVLTGKPY LCDLITDSMV LCFFIDSEKI
LSLQSDSTID DFLWQESALV LLKLLRPQIF ESVAMQELRA LVSTESSKLT TYVTGESIEI
DCNSIGLLLE GFVKPVGIKE ELISSPAALS PSNGNQSFHN SSEASGIMRV SFSQQATQYI
VETRARAIIF NIGAFGADRT LHRRPSSLTP PRSSSSDQLQ RSFRKEHRGL MSWPENIYAK
QQQEINKTTL SLSERAMQLS IFGSMVNVYR RSVSFGGIYN NKLQDNLLYK KLPLNPAQGL
VSAKSESSIV TKKQLETRKH ACQLPLKGES STRQNTMVES SDEEDEDEGI VVRIDSPSKI
VFRNDL
