NHX8_ARATH
ID NHX8_ARATH Reviewed; 756 AA.
AC Q3YL57; Q9LQW7; Q9MA19;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Sodium/hydrogen exchanger 8;
DE AltName: Full=Na(+)/H(+) exchanger 8;
DE Short=NHE-8;
DE AltName: Full=Protein SALT OVERLY SENSITIVE 1B;
GN Name=NHX8; Synonyms=SOS1B; OrderedLocusNames=At1g14660;
GN ORFNames=F10B6.1, T5E21.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA An R., Chen Q.-J., Wang X.-C.;
RT "AtNHX8/SOS1b, a member of the monovalent cation:proton antiporter-1 family
RT from Arabidopsis thaliana, encodes a putative plasma membrane Li(+)/H(+)
RT antiporter.";
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: May act in low affinity electroneutral exchange of protons
CC for cations such as Na(+) or K(+) across membranes. May also exchange
CC Li(+) and Cs(+) with a lower affinity. {ECO:0000250|UniProtKB:Q68KI4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + Na(+)(in) = H(+)(in) + Na(+)(out);
CC Xref=Rhea:RHEA:29419, ChEBI:CHEBI:15378, ChEBI:CHEBI:29101;
CC Evidence={ECO:0000250|UniProtKB:Q68KI4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(out) + K(+)(in) = H(+)(in) + K(+)(out);
CC Xref=Rhea:RHEA:29467, ChEBI:CHEBI:15378, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000250|UniProtKB:Q68KI4};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q68KI4};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q3YL57-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC transporter (TC 2.A.36) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF63173.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF79251.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ104324; AAZ76246.1; -; mRNA.
DR EMBL; AC006917; AAF79251.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC010657; AAF63173.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29198.1; -; Genomic_DNA.
DR RefSeq; NP_172918.2; NM_101333.5. [Q3YL57-1]
DR AlphaFoldDB; Q3YL57; -.
DR SMR; Q3YL57; -.
DR BioGRID; 23268; 7.
DR IntAct; Q3YL57; 7.
DR STRING; 3702.AT1G14660.1; -.
DR TCDB; 2.A.36.7.3; the monovalent cation:proton antiporter-1 (cpa1) family.
DR PaxDb; Q3YL57; -.
DR PRIDE; Q3YL57; -.
DR ProteomicsDB; 249391; -. [Q3YL57-1]
DR EnsemblPlants; AT1G14660.1; AT1G14660.1; AT1G14660. [Q3YL57-1]
DR GeneID; 838028; -.
DR Gramene; AT1G14660.1; AT1G14660.1; AT1G14660. [Q3YL57-1]
DR KEGG; ath:AT1G14660; -.
DR Araport; AT1G14660; -.
DR TAIR; locus:2006762; AT1G14660.
DR eggNOG; KOG1965; Eukaryota.
DR HOGENOM; CLU_005912_8_0_1; -.
DR InParanoid; Q3YL57; -.
DR OMA; VDPRNIM; -.
DR OrthoDB; 99426at2759; -.
DR PhylomeDB; Q3YL57; -.
DR PRO; PR:Q3YL57; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q3YL57; baseline and differential.
DR Genevisible; Q3YL57; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0015385; F:sodium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0010352; P:lithium ion export across the plasma membrane; IMP:TAIR.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR GO; GO:0098719; P:sodium ion import across plasma membrane; IBA:GO_Central.
DR InterPro; IPR006153; Cation/H_exchanger.
DR InterPro; IPR018422; Cation/H_exchanger_CPA1.
DR InterPro; IPR018409; Na/H_exchanger_8.
DR PANTHER; PTHR10110; PTHR10110; 1.
DR PANTHER; PTHR10110:SF165; PTHR10110:SF165; 1.
DR Pfam; PF00999; Na_H_Exchanger; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Antiport; Cell membrane; Glycoprotein; Ion transport;
KW Membrane; Potassium; Potassium transport; Reference proteome; Sodium;
KW Sodium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..756
FT /note="Sodium/hydrogen exchanger 8"
FT /id="PRO_0000052379"
FT TOPO_DOM 1..31
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..56
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 78..89
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..125
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..157
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..194
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..225
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..251
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..271
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 272..276
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..311
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 333..350
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..385
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 386..406
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 407..422
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 423..443
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 444..756
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 756 AA; 83476 MW; EFDE7CC4C55DB026 CRC64;
MTSIIGAALP YKSPEKAIAS SSYSAENDSS PVDAVIFAGT SLVLGTACRY LFNGTRVPYT
VVLLVIGIFL GSLEYGTKHN LGKLGHGIRI WNGINPDLLL AVFLPVLLFE SSFSMDVHQI
KRCMGQMVLL AGPGVLISTF CLGALIKLTF PYNWDWKTSL LLGGLLGATD PVAVVALLKE
LGASKKMTTL IDGESLMNDG VSVVVFQLFF KMVMGHNSDW GSIIKFLVQN SFGAVGIGLA
FGIASVFWLK FIFNDTVAQI TVTLSASYFA YYTAQEWAGV SGILTVMILG MFFAAFARTA
FKGDSHQSLH HFWEMAAYIA NTLVFMLSGV IIAESVLSGQ TISYKGNSWS FLFLLYLYVQ
LSRCVVVGVL YPLLCRSGYG LDWKESIILT WSGLRGAVSL SLALSVKQSS GNSYLSSDTG
TRFLFLTGGI VFLTLVVNGS TTQLLLHLLR MDTLTATKKR ILEYTKFEMM KTALKAFENL
GDDEELGSAD WPTVIRHISS LKDLEGRQVN PHDGYEAGSL DPTNIMDIRI RFLNGVQAAY
WEMLDDGRIT QCTANVLMQS VDEALDLVST SSLSDWRGLE PRVHFPNYYK FLQSKIIPHK
LVTHLIVERL ESACYISSAF LRAHRIARQQ LHIFLGNSNI ASTVINESEV EGEEAKQFLE
DVRDSFPQVL SVLKTRQVTH YVLNHLNGYI KNLEKVGLLE GKEVSHLHDV VQSDLKKLLR
HPPSLKLPNV DDLITSNPLL KDRSSFRSLA IGETDA
