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NIA_USTMA
ID   NIA_USTMA               Reviewed;         983 AA.
AC   Q05531; A0A0D1DXC6; Q4P7R6; Q96VE8;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 150.
DE   RecName: Full=Nitrate reductase [NADPH];
DE            Short=NR;
DE            EC=1.7.1.3;
GN   Name=NAR1; ORFNames=UMAG_03847;
OS   Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC   Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX   NCBI_TaxID=237631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=8370542; DOI=10.1016/0378-1119(93)90670-x;
RA   Banks G.R., Holden D.W., Kanuga N., Shelton P., Spanos A.;
RT   "The Ustilago maydis nar1 gene encoding nitrate reductase activity:
RT   sequence and transcriptional regulation.";
RL   Gene 131:69-78(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=FBD11;
RX   PubMed=11737646; DOI=10.1046/j.1365-2958.2001.02699.x;
RA   Brachmann A., Weinzierl G., Kaemper J., Kahmann R.;
RT   "Identification of genes in the bW/bE regulatory cascade in Ustilago
RT   maydis.";
RL   Mol. Microbiol. 42:1047-1063(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=521 / FGSC 9021;
RX   PubMed=17080091; DOI=10.1038/nature05248;
RA   Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA   Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA   Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA   Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA   Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA   Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA   Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA   Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA   Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA   Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA   Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA   Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA   Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA   Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA   Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA   Birren B.W.;
RT   "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT   maydis.";
RL   Nature 444:97-101(2006).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=521 / FGSC 9021;
RA   Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL   Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Nitrate reductase is a key enzyme involved in the first step
CC       of nitrate assimilation in plants, fungi and bacteria.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + NADP(+) + nitrite = H(+) + NADPH + nitrate;
CC         Xref=Rhea:RHEA:19061, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16301, ChEBI:CHEBI:17632, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349; EC=1.7.1.3;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC       Note=Binds 1 FAD per subunit. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC       Note=Binds 1 heme group per subunit. The heme group is called
CC       cytochrome b-557. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Mo-molybdopterin; Xref=ChEBI:CHEBI:71302; Evidence={ECO:0000250};
CC       Note=Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.
CC       {ECO:0000250};
CC   -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- INDUCTION: Its expression is highly regulated and responds rapidly to
CC       nitrate induction and to ammonium ion repression.
CC   -!- SIMILARITY: Belongs to the nitrate reductase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA47918.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X67687; CAA47918.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AJ315577; CAC41650.1; -; Genomic_DNA.
DR   EMBL; CM003149; KIS68266.1; -; Genomic_DNA.
DR   PIR; JN0804; JN0804.
DR   RefSeq; XP_011390279.1; XM_011391977.1.
DR   AlphaFoldDB; Q05531; -.
DR   SMR; Q05531; -.
DR   STRING; 5270.UM03847P0; -.
DR   EnsemblFungi; KIS68266; KIS68266; UMAG_03847.
DR   GeneID; 23564194; -.
DR   KEGG; uma:UMAG_03847; -.
DR   VEuPathDB; FungiDB:UMAG_03847; -.
DR   eggNOG; KOG0534; Eukaryota.
DR   eggNOG; KOG0535; Eukaryota.
DR   eggNOG; KOG0537; Eukaryota.
DR   HOGENOM; CLU_003827_4_0_1; -.
DR   InParanoid; Q05531; -.
DR   OMA; TAKAMMP; -.
DR   OrthoDB; 166846at2759; -.
DR   UniPathway; UPA00653; -.
DR   Proteomes; UP000000561; Chromosome 10.
DR   GO; GO:0071949; F:FAD binding; ISS:UniProtKB.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0030151; F:molybdenum ion binding; ISS:UniProtKB.
DR   GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR   GO; GO:0050464; F:nitrate reductase (NADPH) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.10.120.10; -; 1.
DR   Gene3D; 3.40.50.80; -; 1.
DR   Gene3D; 3.90.420.10; -; 1.
DR   InterPro; IPR001834; CBR-like.
DR   InterPro; IPR008333; Cbr1-like_FAD-bd_dom.
DR   InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR   InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR   InterPro; IPR018506; Cyt_B5_heme-BS.
DR   InterPro; IPR017927; FAD-bd_FR_type.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR039261; FNR_nucleotide-bd.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR005066; MoCF_OxRdtse_dimer.
DR   InterPro; IPR008335; Mopterin_OxRdtase_euk.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR   InterPro; IPR000572; OxRdtase_Mopterin-bd_dom.
DR   InterPro; IPR036374; OxRdtase_Mopterin-bd_sf.
DR   InterPro; IPR022407; OxRdtase_Mopterin_BS.
DR   InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR   PANTHER; PTHR19370; PTHR19370; 1.
DR   Pfam; PF00173; Cyt-b5; 1.
DR   Pfam; PF00970; FAD_binding_6; 1.
DR   Pfam; PF03404; Mo-co_dimer; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   Pfam; PF00174; Oxidored_molyb; 1.
DR   PRINTS; PR00363; CYTOCHROMEB5.
DR   PRINTS; PR00407; EUMOPTERIN.
DR   PRINTS; PR00371; FPNCR.
DR   SMART; SM01117; Cyt-b5; 1.
DR   SUPFAM; SSF52343; SSF52343; 1.
DR   SUPFAM; SSF55856; SSF55856; 1.
DR   SUPFAM; SSF56524; SSF56524; 1.
DR   SUPFAM; SSF63380; SSF63380; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
DR   PROSITE; PS00191; CYTOCHROME_B5_1; 1.
DR   PROSITE; PS50255; CYTOCHROME_B5_2; 1.
DR   PROSITE; PS51384; FAD_FR; 1.
DR   PROSITE; PS00559; MOLYBDOPTERIN_EUK; 1.
PE   2: Evidence at transcript level;
KW   FAD; Flavoprotein; Heme; Iron; Metal-binding; Molybdenum; NADP;
KW   Nitrate assimilation; Oxidoreductase; Reference proteome.
FT   CHAIN           1..983
FT                   /note="Nitrate reductase [NADPH]"
FT                   /id="PRO_0000166048"
FT   DOMAIN          585..662
FT                   /note="Cytochrome b5 heme-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT   DOMAIN          688..815
FT                   /note="FAD-binding FR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00716"
FT   REGION          1..50
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         184
FT                   /ligand="Mo-molybdopterin"
FT                   /ligand_id="ChEBI:CHEBI:71302"
FT                   /ligand_part="Mo"
FT                   /ligand_part_id="ChEBI:CHEBI:28685"
FT                   /evidence="ECO:0000250|UniProtKB:P49050"
FT   BINDING         622
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT   BINDING         645
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT   BINDING         746..749
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A0A286R227"
FT   BINDING         763..767
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A0A286R227"
FT   BINDING         768
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:P17571"
FT   BINDING         780
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A0A286R227"
FT   BINDING         784..786
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A0A286R227"
FT   BINDING         841
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:P17571"
FT   BINDING         844
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A0A286R227"
FT   BINDING         952..961
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        5
FT                   /note="T -> S (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        18..20
FT                   /note="KGD -> NP (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        49
FT                   /note="V -> I (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        136
FT                   /note="H -> Q (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        178..179
FT                   /note="LP -> PT (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        243..244
FT                   /note="PG -> LD (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        306
FT                   /note="G -> S (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        333
FT                   /note="K -> Q (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        365
FT                   /note="A -> S (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        387
FT                   /note="Q -> E (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        414
FT                   /note="H -> R (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        463
FT                   /note="Q -> E (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        482
FT                   /note="G -> V (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        486
FT                   /note="M -> Q (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        506
FT                   /note="Missing (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        549
FT                   /note="G -> V (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        572..573
FT                   /note="VD -> WV (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        612
FT                   /note="V -> L (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        636
FT                   /note="D -> DD (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        673..675
FT                   /note="TEG -> ER (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        726..733
FT                   /note="HVFVRVRS -> QLCVCRL (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        741
FT                   /note="T -> A (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        894..895
FT                   /note="LR -> M (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        927
FT                   /note="T -> S (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        970..971
FT                   /note="KQ -> NE (in Ref. 1; CAA47918)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   983 AA;  108826 MW;  E8BF3D172EEB69B9 CRC64;
     MTISTTSSST SSKTSSEKGD DLKGFSSSSS PASSRSSSAT TPEPSSPTVL AAKTIESIDP
     FQPRKDYNEN VLPAIPATEA VQPLTSDANT PDHWIARDER MIRLTGKHPF NSEAPLSELF
     SKGFLTPQNL FYVRSHGDTP RVTREQAENW KLKVHGLVEQ EVELSIKDLK EKFPTVTLPI
     TLVCAGNRRK EQNMVAKGLG FNWGAAGVST GLFTGVYLAD ILDYCKPKNP LLSSFPSYDV
     AVPGRARHVV FEGADELPKG KYGTSQRLNW ALDRCKGMLI AWGLNGEDLS PDHGYPLRLV
     VPGQIGGRMV KWLERIEVSD RESQHHLHFH DNKVLPTEVT ADQARSEMHW WYDPKYIIND
     LNVNAAICSP DHDQVVNVAE PSTSSPQMLP LEGYAYTGGG RRIHRVEISL DDGHSWKCAS
     IHYPEDLYRM YPIQGHEYFG TLDLSATEMS FSWCFWRLDV DVQADIIAKD VKVISVRALD
     EGLATMPRDM YWNATSMMNS WWFRVAVHRE GENGNQIRFE HPTLAGNAPG GWMQRMNEAG
     LNPRYPQFGE AKAVESCKTD ANTLAAAKEA KVDPKAIMID PSKADTIITA ADLAAHGDGE
     GPEPWFVVHG HVYDGTGFLK DHPGGDQSIR LVAGEDATED FMAIHSMDAK KMLRDFHLGR
     LEKQDAAPPA ATTEGEVLDL SKPFLDPKKW RATRLVSKQI ISPDARIFRF ALGSEDQELG
     LPVGQHVFVR VRSKNARTGE TEMVQRAYTP YSGNTQRGFL DILIKVYFPS DAAATSAPAF
     EGGKMTMLLE KIDVSSPSDD LTIELKGPLG SFTYLGQQQI RWKPASAVRR VRKLAMIAGG
     SGITPIWSTL KAIADEVLDA SNPSSPALDP IQIWIVYGNR TEQDILIREE LERLRVALKG
     NLKVWHVLSN CTPENEANWS MGRGHITANV LRTHLPPPPA KPASEDELED TLALVCGPPP
     MEKAVSDGLK QLGWDLQRCV VFF
 
 
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