NIC2_ARATH
ID NIC2_ARATH Reviewed; 198 AA.
AC Q9FMX7;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Nicotinamidase 2 {ECO:0000305};
DE Short=AtNIC2 {ECO:0000303|PubMed:17587307};
DE EC=3.5.1.19 {ECO:0000269|PubMed:17587307};
DE AltName: Full=Nicotinamide deamidase 2;
GN Name=NIC2 {ECO:0000303|PubMed:17587307};
GN OrderedLocusNames=At5g23230 {ECO:0000312|Araport:AT5G23230};
GN ORFNames=MKD15.9 {ECO:0000312|EMBL:BAB11178.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17587307; DOI=10.1111/j.1365-313x.2007.03151.x;
RA Hunt L., Holdsworth M.J., Gray J.E.;
RT "Nicotinamidase activity is important for germination.";
RL Plant J. 51:341-351(2007).
CC -!- FUNCTION: Catalyzes the deamidation of nicotinamide, an early step in
CC the NAD(+) salvage pathway. Prevents the accumulation of intracellular
CC nicotinamide, a known inhibitor of poly(ADP-ribose) polymerases (PARP
CC enzymes). May play a role in the regulation of seed germination
CC potential. {ECO:0000269|PubMed:17587307}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + nicotinamide = NH4(+) + nicotinate;
CC Xref=Rhea:RHEA:14545, ChEBI:CHEBI:15377, ChEBI:CHEBI:17154,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:32544; EC=3.5.1.19;
CC Evidence={ECO:0000269|PubMed:17587307};
CC -!- PATHWAY: Cofactor biosynthesis; nicotinate biosynthesis; nicotinate
CC from nicotinamide: step 1/1. {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed during seed germination.
CC {ECO:0000269|PubMed:17587307}.
CC -!- DISRUPTION PHENOTYPE: Delayed seed germination, reduced seed
CC germination potential and increased sensitivity to abscisic acid (ABA)
CC during germination. {ECO:0000269|PubMed:17587307}.
CC -!- SIMILARITY: Belongs to the isochorismatase family. {ECO:0000305}.
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DR EMBL; AB007648; BAB11178.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93139.1; -; Genomic_DNA.
DR EMBL; BT004805; AAO44071.1; -; mRNA.
DR EMBL; AK227782; BAE99764.1; -; mRNA.
DR RefSeq; NP_197714.1; NM_122229.4.
DR AlphaFoldDB; Q9FMX7; -.
DR SMR; Q9FMX7; -.
DR IntAct; Q9FMX7; 1.
DR STRING; 3702.AT5G23230.1; -.
DR PaxDb; Q9FMX7; -.
DR PRIDE; Q9FMX7; -.
DR ProteomicsDB; 249387; -.
DR EnsemblPlants; AT5G23230.1; AT5G23230.1; AT5G23230.
DR GeneID; 832387; -.
DR Gramene; AT5G23230.1; AT5G23230.1; AT5G23230.
DR KEGG; ath:AT5G23230; -.
DR Araport; AT5G23230; -.
DR TAIR; locus:2166958; AT5G23230.
DR eggNOG; ENOG502QQB1; Eukaryota.
DR HOGENOM; CLU_068979_8_3_1; -.
DR InParanoid; Q9FMX7; -.
DR OMA; IIIDMQT; -.
DR OrthoDB; 1344853at2759; -.
DR PhylomeDB; Q9FMX7; -.
DR UniPathway; UPA00830; UER00790.
DR PRO; PR:Q9FMX7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMX7; differential.
DR Genevisible; Q9FMX7; AT.
DR GO; GO:0008936; F:nicotinamidase activity; IDA:TAIR.
DR GO; GO:0019674; P:NAD metabolic process; IMP:TAIR.
DR GO; GO:0019363; P:pyridine nucleotide biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.850; -; 1.
DR InterPro; IPR000868; Isochorismatase-like.
DR InterPro; IPR036380; Isochorismatase-like_sf.
DR Pfam; PF00857; Isochorismatase; 1.
DR SUPFAM; SSF52499; SSF52499; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Pyridine nucleotide biosynthesis; Reference proteome.
FT CHAIN 1..198
FT /note="Nicotinamidase 2"
FT /id="PRO_0000431488"
SQ SEQUENCE 198 AA; 22318 MW; F0A53BC468F208A1 CRC64;
MASSSSRTYE TRKREPNPKI AALLVIDMQN HFYSMAEPIL QNALTTIDIC RRASIPVFFT
RHNHKSPTDH GMLGEWWNGD LILDGTTDSE IIPEINRQVT GPDEIVEKST YSAFNNTHLQ
EKLDKIGVKE VIVIGVMTNL CCETTAREAF VKGFRVFFST DATATVNEEL HEATLMNLAY
GFAYLVDCDR LRRGLLSS
